[논문]Hydrangenol inhibits lipopolysaccharide-induced nitric oxide production in BV2 microglial cells by suppressing the NF-κB pathway and activating the Nrf2-mediated HO-1 pathway

Kim, Hee-Ju; Kang, Chang-Hee; Jayasooriya, Rajapaksha Gedara Prasad Tharanga; Dilshara, Matharage Gayani; Lee, Seungheon; Choi, Yung Hyun; Seo, Yong Taek; Kim, Gi-Young

doi:10.1016/j.intimp.2016.03.022

[해외논문] Hydrangenol inhibits lipopolysaccharide-induced nitric oxide production in BV2 microglial cells by suppressing the NF-κB pathway and activating the Nrf2-mediated HO-1 pathway

International immunopharmacology, v.35, 2016년, pp.61 - 69

Kim, Hee-Ju (Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea) , Kang, Chang-Hee (Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea) , Jayasooriya, Rajapaksha Gedara Prasad Tharanga (Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea) , Dilshara, Matharage Gayani (Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea) , Lee, Seungheon (Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea) , Choi, Yung Hyun (Department of Biochemistry, College of Oriental Medicine, Dong-Eui University, Busan 47227, Republic of Korea) , Seo, Yong Taek (Consilience Medical Center, Dankook University Hospital, Chungcheongnam-do 31116, Republic of Korea) , Kim, Gi-Young (Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea)

Abstract ▼ AI-Helper

We previously demonstrated the anti-inflammatory effect of water extract of Hydrangea macrophylla in lipopolysaccharide (LPS)-stimulated macrophage cells. Here, we investigated whether hydrangenol, a bioactive component of H. macrophylla, attenuates the expression of nitric oxide (NO) and its associated gene, inducible NO synthase (iNOS), in LPS-stimulated BV2 microglial cells. Our data showed that low dosages of hydrangenol inhibited LPS-stimulated NO release and iNOS expression without any accompanying cytotoxicity. Hydrangenol also suppressed LPS-induced nuclear translocation of nuclear factor-kappa B (NF-kappa B) subunits, consequently inhibiting DNA-binding activity of NF-kappa B. Additionally, the NF-kappa B inhibitors, pyrrolidine dithiocarbamate (PDTC) and PS-1145, significantly attenuated LPS-induced iNOS expression, indicating that hydrangenol-induced NF-kappa B inhibition might be a key regulator of iNOS expression. Furthermore, our data showed that hydrangenol suppresses NO production by inducing heme oxygenase-1 (HO-1). The presence of cobalt protoporphyrin, a specific HO-1 inducer, potently suppressed LPS-induced NO production. Hydrangenol also promoted nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and subsequently increased its binding activity at the specific antioxidant response element sites. Additionally, transient knockdown of Nrf2 significantly downregulated hydrangenol-induced HO-1 expression, indicating that hydrangenol-induced Nrf2 is an upstream regulator of HO-1. Taken together, these data suggest that hydrangenol attenuates NO production and iNOS expression in LPS-stimulated BV2 microglial cells by inhibiting NF-kappa B activation and by stimulating the Nrf2-mediated HO-1 signaling pathway. Therefore, hydrangenol is a promising therapeutic agent for treatment of LPS-mediated inflammatory diseases. (C) 2016 Elsevier B.V. All rights reserved.

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참고문헌 (27)

Pharmaceuticals Nakagawa 7 1028 2014 10.3390/ph7121028 Role of microglial m1/m2 polarization in relapse and remission of psychiatric disorders and diseases

상세보기
J. Leukoc. Biol. Jin 87 779 2010 10.1189/jlb.1109766 Inflammatory mechanisms in ischemic stroke: role of inflammatory cells

상세보기
Int. J. Alzheimers Dis. Noda 2012 891087 2012 Sweepers in the CNS: microglial migration and phagocytosis in the Alzheimer disease pathogenesis
Clin. Dev. Immunol. Goldmann 2013 208093 2013 10.1155/2013/208093 Role of microglia in CNS autoimmunity

상세보기
Exp. Neurol. Garry 263 235 2015 10.1016/j.expneurol.2014.10.017 The role of the nitric oxide pathway in brain injury and its treatment - from bench to bedside

상세보기
PLoS One Jiang 9 2014 Role of nitric oxide synthases in early blood-brain barrier disruption following transient focal cerebral ischemia

상세보기
Neuroscience Wang 281C 164 2014 10.1016/j.neuroscience.2014.09.048 Nitric oxide plays a dual role in the oxidative injury of cultured rat microglia but not astroglia

상세보기
Free Radic. Biol. Med. Kumar 73 51 2014 10.1016/j.freeradbiomed.2014.04.014 Inducible nitric oxide synthase is key to peroxynitrite-mediated, LPS-induced protein radical formation in murine microglial BV2 cells

상세보기
J. Mol. Neurosci. Li 48 225 2012 10.1007/s12031-012-9814-5 Neuroprotection by silencing iNOS expression in a 6-OHDA model of Parkinson's disease

상세보기
Evid. Based Complement. Alternat. Med. de Melo 2014 960258 2014 10.1155/2014/960258 A systematic review for anti-inflammatory property of clusiaceae family: a preclinical approach

상세보기
Biochem. Pharmacol. Verstrepen 92 519 2014 10.1016/j.bcp.2014.10.017 Receptor proximal kinases in NF-κB signaling as potential therapeutic targets in cancer and inflammation

상세보기
Food Funct. Duarte 5 1653 2014 10.1039/C4FO00144C Modulation of nitric oxide by flavonoids

상세보기
Trans. Am. Clin. Climatol. Assoc. Agarwal 124 111 2013 Adaptive responses to tissue injury: role of heme oxygenase-1
Am. J. Cardiovasc. Dis. Wu 1 150 2011 Heme oxygenase-1 in inflammation and cardiovascular disease

상세보기
Chem. Pharm. Bull. Liu 61 655 2013 10.1248/cpb.c13-00160 Medicinal flowers. XXXX. Structures of dihydroisocoumarin glycosides and inhibitory effects on aldose reductase from the flowers of Hydrangea macrophylla var. thunbergii

상세보기
Structure Jain 23 819 2015 10.1016/j.str.2015.02.011 Structure of prolyl-tRNA synthetase-halofuginone complex provides basis for development of drugs against malaria and toxoplasmosis

상세보기
Nat. Chem. Biol. Keller 8 311 2012 10.1038/nchembio.790 Halofuginone and other febrifugine derivatives inhibit prolyl-tRNA synthetase

상세보기
Environ. Toxicol. Pharmacol. Dilshara 35 311 2013 10.1016/j.etap.2012.12.012 Water extract of processed Hydrangea macrophylla (Thunb.) Ser. leaf attenuates the expression of pro-inflammatory mediators by suppressing Akt-mediated NF-κB activation

상세보기
Acta Med. Okayama Kamei 54 227 2000 Anti-malarial activity of leaf-extract of Hydrangea macrophylla, a common Japanese plant

상세보기
Yakugaku Zasshi Nakamura 131 909 2011 10.1248/yakushi.131.909 Search for antidiabetic constituents of medicinal food

상세보기
J. Pharm. Bioallied Sci. Gulati 6 233 2014 10.4103/0975-7406.142951 Pharmacological evidence for connection of nitric oxide-mediated pathways in neuroprotective mechanism of ischemic postconditioning in mice

상세보기
Biochim. Biophys. Acta Okamoto 1850 1588 2015 10.1016/j.bbagen.2014.12.013 S-nitrosylation in neurogenesis and neuronal development

상세보기
Future Med. Chem. Zhou 5 1559 2013 10.4155/fmc.13.125 Anti-neuroinflammatory agents for the treatment of Alzheimer's disease

상세보기
Biomed. Res. Int. Bortolotto 2014 612798 2014 10.1155/2014/612798 NF-κB mediated regulation of adult hippocampal neurogenesis: relevance to mood disorders and antidepressant activity

상세보기
Curr. Drug Targets Jazwa 11 1517 2010 10.2174/1389450111009011517 Targeting heme oxygenase-1 for neuroprotection and neuroinflammation in neurodegenerative diseases

상세보기
J. Neurosci. Vargas 28 13574 2008 10.1523/JNEUROSCI.4099-08.2008 Nrf2 activation in astrocytes protects against neurodegeneration in mouse models of familial amyotrophic lateral sclerosis

상세보기
Cell. Signal. Yu 23 883 2011 10.1016/j.cellsig.2011.01.014 Nuclear factor p65 interacts with Keap1 to repress the Nrf2-ARE pathway

상세보기

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[해외논문] Hydrangenol inhibits lipopolysaccharide-induced nitric oxide production in BV2 microglial cells by suppressing the NF-κB pathway and activating the Nrf2-mediated HO-1 pathway

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[해외논문] Hydrangenol inhibits lipopolysaccharide-induced nitric oxide production in BV2 microglial cells by suppressing the NF-κB pathway and activating the Nrf2-mediated HO-1 pathway

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