[논문]Quorum sensing is crucial to Escherichia coli O157:H7 biofilm formation under static or very slow laminar flow conditions

Lim, Jeesun; Lee, Kang-Mu; Park, Chan Yong; Kim, Han Vit; Kim, Younghoon; Park, Sungsu

doi:10.1007/s13206-016-0310-9

Quorum sensing is crucial to Escherichia coli O157:H7 biofilm formation under static or very slow laminar flow conditions 원문보기

Biochip journal, v.10 no.3, 2016년, pp.241 - 249

Lim, Jeesun , Lee, Kang-Mu , Park, Chan Yong , Kim, Han Vit , Kim, Younghoon , Park, Sungsu

Abstract ▼ AI-Helper

Quorum sensing (QS) is defined as a cell to-cell signaling process that collectively regulates the gene expression of bacteria via small signaling molecules called autoinducers (AIs). It was reported that QS-regulated gene expression in Pseudomonas aeruginosa failed to occur at a high Reynolds number (Re= 3,000), since AI-2, a secreted interspecies signaling molecule, was washed away and so could not reach the minimum concentration required for QS. In this study, we describe the effects of flow speed on QS-stimulated biofilm formation in Escherichia coli O157:H7 inside a very thin microchannel (3 cm x 1 cm x 45 mu m). In micro titer plates, the wild-type strain produced high amounts of exopolysaccharide, whereas its QS mutants Delta luxS and Delta lsrK, defective in AI-2 production and phosphorylation, made less exopolysaccharide. Confocal laser scanning microscopy showed that at a flow rate of 1 mu L/min the wild-type strain formed rounded biofilms, whereas such biofilms formed by the QS mutants were fewer and thinner. At a flow rate of 10 mu L/min, none of the tested strains formed mature biofilms. Our results suggest that QS is essential in the biofilm maturation under static or very slow laminar flow conditions where the biofilm signal can be easily accumulated and transported to the sessile cells.

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Quorum sensing is crucial to Escherichia coli O157:H7 biofilm formation under static or very slow laminar flow conditions 원문보기

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Quorum sensing is crucial to Escherichia coli O157:H7 biofilm formation under static or very slow laminar flow conditions 원문보기

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