$\require{mediawiki-texvc}$

연합인증

연합인증 가입 기관의 연구자들은 소속기관의 인증정보(ID와 암호)를 이용해 다른 대학, 연구기관, 서비스 공급자의 다양한 온라인 자원과 연구 데이터를 이용할 수 있습니다.

이는 여행자가 자국에서 발행 받은 여권으로 세계 각국을 자유롭게 여행할 수 있는 것과 같습니다.

연합인증으로 이용이 가능한 서비스는 NTIS, DataON, Edison, Kafe, Webinar 등이 있습니다.

한번의 인증절차만으로 연합인증 가입 서비스에 추가 로그인 없이 이용이 가능합니다.

다만, 연합인증을 위해서는 최초 1회만 인증 절차가 필요합니다. (회원이 아닐 경우 회원 가입이 필요합니다.)

연합인증 절차는 다음과 같습니다.

최초이용시에는
ScienceON에 로그인 → 연합인증 서비스 접속 → 로그인 (본인 확인 또는 회원가입) → 서비스 이용

그 이후에는
ScienceON 로그인 → 연합인증 서비스 접속 → 서비스 이용

연합인증을 활용하시면 KISTI가 제공하는 다양한 서비스를 편리하게 이용하실 수 있습니다.

Mammalian Systems Biotechnology Reveals Global Cellular Adaptations in a Recombinant CHO Cell Line 원문보기

Cell systems, v.4 no.5, 2017년, pp.530 - 542.e6  

Yusufi, F.N.K. ,  Lakshmanan, M. ,  Ho, Y.S. ,  Loo, B.L.W. ,  Ariyaratne, P. ,  Yang, Y. ,  Ng, S.K. ,  Tan, T.R.M. ,  Yeo, H.C. ,  Lim, H.L. ,  Ng, S.W. ,  Hiu, A.P. ,  Chow, C.P. ,  Wan, C. ,  Chen, S. ,  Teo, G. ,  Song, G. ,  Chin, J.X. ,  Ruan, X. ,  Sung, K.W.K. ,  Hu, W.S. ,  Yap, M.G.S. ,  Bardor, M. ,  Nagarajan, N. ,  Lee, D.Y.

Abstract AI-Helper 아이콘AI-Helper

Effective development of host cells for therapeutic protein production is hampered by the poor characterization of cellular transfection. Here, we employed a multi-omics-based systems biotechnology approach to elucidate the genotypic and phenotypic differences between a wild-type and recombinant ant...

주제어

참고문헌 (72)

  1. Biotechnology Alt 24 397 1992 Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells. 1978 

  2. J. Mol. Biol. Altschul 215 403 1990 10.1016/S0022-2836(05)80360-2 Basic local alignment search tool 

  3. Biotechnol. Bioeng. Baik 93 361 2006 10.1002/bit.20717 Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin 

  4. Oncogene Barjesteh van Waalwijk van Doorn-Khosrovani 24 4129 2005 10.1038/sj.onc.1208588 Somatic heterozygous mutations in ETV6 (TEL) and frequent absence of ETV6 protein in acute myeloid leukemia 

  5. PLoS Comput. Biol. Becker 4 e1000082 2008 10.1371/journal.pcbi.1000082 Context-specific metabolic networks are consistent with experiments 

  6. PLoS One Bedoya-López 11 e0151529 2016 10.1371/journal.pone.0151529 Effect of temperature downshift on the transcriptomic responses of Chinese hamster ovary cells using recombinant human tissue plasminogen activator production culture 

  7. Nucleic Acids Res. Birzele 38 3999 2010 10.1093/nar/gkq116 Into the unknown: expression profiling without genome sequence information in CHO by next generation sequencing 

  8. Bioinformatics Bolger 30 2114 2014 10.1093/bioinformatics/btu170 Trimmomatic: a flexible trimmer for Illumina sequence data 

  9. Nat. Biotechnol. Brinkrolf 31 694 2013 10.1038/nbt.2645 Chinese hamster genome sequenced from sorted chromosomes 

  10. Aging Cell Busuttil 2 287 2003 10.1046/j.1474-9728.2003.00066.x Oxygen accelerates the accumulation of mutations during the senescence and immortalization of murine cells in culture 

  11. Trends Biotechnol. Carinhas 30 377 2012 10.1016/j.tibtech.2012.03.004 Systems biotechnology of animal cells: the road to prediction 

  12. Brief. Bioinform. Cavill 17 891 2016 10.1093/bib/bbv090 Transcriptomic and metabolomic data integration 

  13. Biotechnol. Bioeng. Charaniya 102 1654 2009 10.1002/bit.22210 Mining transcriptome data for function-trait relationship of hyper productivity of recombinant antibody 

  14. Clin. Exp. Immunol. Chen 179 188 2015 10.1111/cei.12447 α-Galactosylceramide stimulates splenic lymphocyte proliferation in vitro and increases antibody production in vivo in late neonatal-age mice 

  15. Rapid Commun. Mass Spectrom. Chong 23 3763 2009 10.1002/rcm.4328 Metabolomics profiling of extracellular metabolites in recombinant Chinese Hamster Ovary fed-batch culture 

  16. J. Biotechnol. Chong 151 218 2011 10.1016/j.jbiotec.2010.12.010 Metabolomics-based identification of apoptosis-inducing metabolites in recombinant fed-batch CHO culture media 

  17. Biotechnol. Bioeng. Chong 109 3103 2012 10.1002/bit.24580 LC-MS-based metabolic characterization of high monoclonal antibody-producing Chinese hamster ovary cells 

  18. J. Biotechnol. Clarke 155 350 2011 10.1016/j.jbiotec.2011.07.011 Large scale microarray profiling and coexpression network analysis of CHO cells identifies transcriptional modules associated with growth and productivity 

  19. Biotechnol. Bioeng. Datta 110 1255 2013 10.1002/bit.24841 An ’omics approach towards CHO cell engineering 

  20. Biotechnol. Prog. del Val 26 1505 2010 10.1002/btpr.470 Towards the implementation of quality by design to the production of therapeutic monoclonal antibodies with desired glycosylation patterns 

  21. Nat. Protoc. Dunn 6 1060 2011 10.1038/nprot.2011.335 Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry 

  22. J. Lipid Res. Fahy 46 839 2005 10.1194/jlr.E400004-JLR200 A comprehensive classification system for lipids 

  23. J. Proteome Res. Farrell 13 3144 2014 10.1021/pr500219b Application of multi-omics techniques for bioprocess design and optimization in Chinese hamster ovary cells 

  24. J. Biol. Chem. Federspiel 259 9127 1984 10.1016/S0021-9258(17)47275-9 Novel DNA rearrangements are associated with dihydrofolate reductase gene amplification 

  25. J. Biotechnol. Fomina-Yadlin 212 106 2015 10.1016/j.jbiotec.2015.08.025 Transcriptome analysis of a CHO cell line expressing a recombinant therapeutic protein treated with inducers of protein expression 

  26. Genome Biol. Gao 17 102 2016 10.1186/s13059-016-0951-y OPERA-LG: efficient and exact scaffolding of large, repeat-rich eukaryotic genomes with performance guarantees 

  27. Biochim. Biophys. Acta Gómez-Muñoz 1758 2049 2006 10.1016/j.bbamem.2006.05.011 Ceramide 1-phosphate/ceramide, a switch between life and death 

  28. Cell Syst. Hefzi 3 434 2016 10.1016/j.cels.2016.10.020 A consensus genome-scale reconstruction of Chinese hamster ovary cell metabolism 

  29. Genome Res. Hillmer 21 665 2011 10.1101/gr.113555.110 Comprehensive long-span paired-end-tag mapping reveals characteristic patterns of structural variations in epithelial cancer genomes 

  30. J. Biotechnol. Ho 157 130 2012 10.1016/j.jbiotec.2011.09.023 IRES-mediated Tricistronic vectors for enhancing generation of high monoclonal antibody expressing CHO cell lines 

  31. Genome Biol. Huang 8 R183 2007 10.1186/gb-2007-8-9-r183 The DAVID Gene Functional Classification Tool: a novel biological module-centric algorithm to functionally analyze large gene lists 

  32. Chem. Eng. Prog. Jayapal 103 40 2007 Recombinant protein therapeutics from CHO cells-20 years and counting 

  33. BMC Genomics Kaas 16 160 2015 10.1186/s12864-015-1391-x Sequencing the CHO DXB11 genome reveals regional variations in genomic stability and haploidy 

  34. J. Biotechnol. Kantardjieff 145 143 2010 10.1016/j.jbiotec.2009.09.008 Transcriptome and proteome analysis of Chinese hamster ovary cells under low temperature and butyrate treatment 

  35. Curr. Opin. Biotechnol. Kildegaard 24 1102 2013 10.1016/j.copbio.2013.02.007 The emerging CHO systems biology era: harnessing the ’omics revolution for biotechnology 

  36. Nat. Methods Kind 10 755 2013 10.1038/nmeth.2551 LipidBlast in silico tandem mass spectrometry database for lipid identification 

  37. Brief. Bioinform. Lakshmanan 15 108 2014 10.1093/bib/bbs069 Software applications for flux balance analysis 

  38. Metabolomics Lee 9 1301 2013 10.1007/s11306-013-0539-4 Precursor mass prediction by clustering ionization products in LC-MS-based metabolomics 

  39. Nat. Biotechnol. Lewis 31 759 2013 10.1038/nbt.2624 Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome 

  40. Bioinformatics Li 26 589 2010 10.1093/bioinformatics/btp698 Fast and accurate long-read alignment with Burrows-Wheeler transform 

  41. Bioinformatics Li 25 2078 2009 10.1093/bioinformatics/btp352 The sequence alignment/map format and SAMtools 

  42. Genome Res. Li 20 265 2010 10.1101/gr.097261.109 De novo assembly of human genomes with massively parallel short read sequencing 

  43. Adv. Cancer Res. Liu 117 59 2013 10.1016/B978-0-12-394274-6.00003-0 Ceramide glycosylation catalyzed by glucosylceramide synthase and cancer drug resistance 

  44. Genome Biol. Nagarajan 13 R115 2012 10.1186/gb-2012-13-12-r115 Whole-genome reconstruction and mutational signatures in gastric cancer 

  45. PLoS One Ng 7 e52785 2012 10.1371/journal.pone.0052785 Production of functional soluble Dectin-1 Glycoprotein using an IRES-linked destabilized-dihydrofolate reductase expression vector 

  46. Cell O’Brien 161 971 2015 10.1016/j.cell.2015.05.019 Using genome-scale models to predict biological capabilities 

  47. J. Proteome Res. Orellana 14 609 2015 10.1021/pr501027c High-antibody-producing Chinese hamster ovary cells up-regulate intracellular protein transport and glutathione synthesis 

  48. J. Biosci. Bioeng. Park 109 504 2010 10.1016/j.jbiosc.2009.10.019 Identification and analysis of specific chromosomal region adjacent to exogenous Dhfr-amplified region in Chinese hamster ovary cell genome 

  49. Proc. Natl. Acad. Sci. USA Patil 102 2685 2005 10.1073/pnas.0406811102 Uncovering transcriptional regulation of metabolism by using metabolic network topology 

  50. Nucleic Acids Res. Pfaffl 29 e45 2001 10.1093/nar/29.9.e45 A new mathematical model for relative quantification in real-time RT-PCR 

  51. Diagn. Mol. Pathol. Rafi 9 184 2000 10.1097/00019606-200012000-00002 ETV6/CBFA2 fusions in childhood B-cell precursor acute lymphoblastic leukemia with myeloid markers 

  52. Methods Mol. Biol. Ruan 809 535 2012 10.1007/978-1-61779-376-9_35 Genome wide full-length transcript analysis using 5′ and 3′ paired-end-tag next generation sequencing (RNA-PET) 

  53. Biotechnol. Bioeng. Schaub 105 431 2010 10.1002/bit.22549 CHO gene expression profiling in biopharmaceutical process analysis and design 

  54. J. Biol. Chem. Schellenberger 284 5457 2009 10.1074/jbc.R800048200 Use of randomized sampling for analysis of metabolic networks 

  55. Nat. Protoc. Schellenberger 6 1290 2011 10.1038/nprot.2011.308 Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox v2.0 

  56. Biotechnol. Bioeng. Sellick 108 3025 2011 10.1002/bit.23269 Metabolite profiling of recombinant CHO cells: designing tailored feeding regimes that enhance recombinant antibody production 

  57. Biotechnol. Bioeng. Selvarasu 109 1415 2012 10.1002/bit.24445 Combined in silico modeling and metabolomics analysis to characterize fed-batch CHO cell culture 

  58. Trends Biotechnol. Sha 34 835 2016 10.1016/j.tibtech.2016.02.013 N-glycosylation design and control of therapeutic monoclonal antibodies 

  59. Anal. Chem. Smith 78 779 2006 10.1021/ac051437y XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification 

  60. J. R. Stat. Soc. Series B Stat. Methodol. Storey 64 479 2002 10.1111/1467-9868.00346 A direct approach to false discovery rates 

  61. BMC Bioinformatics Tautenhahn 9 504 2008 10.1186/1471-2105-9-504 Highly sensitive feature detection for high resolution LC/MS 

  62. Nat. Biotechnol. Trapnell 28 511 2010 10.1038/nbt.1621 Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation 

  63. Nat. Protoc. Trapnell 7 562 2012 10.1038/nprot.2012.016 Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks 

  64. Proteomics Varki 9 5398 2009 10.1002/pmic.200900708 Symbol nomenclature for glycan representation 

  65. Biotechnol. Bioeng. Vishwanathan 112 965 2015 10.1002/bit.25513 Global insights into the Chinese hamster and CHO cell transcriptomes 

  66. Nat. Biotechnol. Walsh 32 992 2014 10.1038/nbt.3040 Biopharmaceutical benchmarks 2014 

  67. PLoS One Wilkens 10 e0119053 2015 10.1371/journal.pone.0119053 Comparative metabolic analysis of CHO cell clones obtained through cell engineering, for IgG productivity, growth and cell longevity 

  68. Nat. Biotechnol. Wurm 22 1393 2004 10.1038/nbt1026 Production of recombinant protein therapeutics in cultivated mammalian cells 

  69. Nat. Biotechnol. Xu 29 735 2011 10.1038/nbt.1932 The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line 

  70. Bioinformatics Ye 25 2865 2009 10.1093/bioinformatics/btp394 Pindel: a pattern growth approach to detect break points of large deletions and medium sized insertions from paired-end short reads 

  71. Biotechnol. Bioeng. Yee 102 246 2009 10.1002/bit.22039 Comparative transcriptome analysis to unveil genes affecting recombinant protein productivity in mammalian cells 

  72. Metabolomics Yeo 12 5 2016 10.1007/s11306-015-0884-6 A genetic algorithm-based approach for pre-processing metabolomics and lipidomics LC-MS data 

LOADING...

관련 콘텐츠

오픈액세스(OA) 유형

GOLD(Hybrid)

저자가 APC(Article Processing Charge)를 지불한 논문에 한하여 자유로운 이용이 가능한, hybrid 저널에 출판된 논문

저작권 관리 안내
섹션별 컨텐츠 바로가기

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

AI-Helper 아이콘
AI-Helper
안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

선택된 텍스트

맨위로