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Fluorescence detection of the transglycosylation activity of amylosucrase

Analytical biochemistry, v.532, 2017년, pp.19 - 25  

Seo, Dong-Ho (Research Group of Gut Microbiome, Korea Food Research Institute, Seongnam 13539, South Korea) ,  Jung, Jong-Hyun (Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, South Korea) ,  Park, Cheon-Seok (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, South Korea)

Abstract AI-Helper 아이콘AI-Helper

The purpose of this study was to investigate the novel fluorescence-based assay for the trans-glycosylation activity of amylosucrase (ASase). The transglycosylation activity of ASase from Deinococcus geothermalis (DGAS), ASase from Neisseria polysaccharea (NPAS), and DGAS-B (chimeric ASase wherein t...

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