[논문]Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina

Jeong, Seok Won; Nam, Seung Won; HwangBo, Kwon; Jeong, Won Joong; Jeong, Byeong-ryool; Chang, Yong Keun; Park, Youn-Il

doi:10.1038/s41598-017-05684-4

Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina 원문보기

Scientific reports, v.7, 2017년, pp.5264 -

Jeong, Seok Won (Department of Biological Sciences, Chungnam National University, Daejeon, 34134 Korea) , Nam, Seung Won (Bioresources Culture Collection Division, Nakdonggang National Institute of Biological Resources, Sangju, 37242 Korea) , HwangBo, Kwon (Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141 Korea) , Jeong, Won Joong (Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141 Korea) , Jeong, Byeong-ryool (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141 Korea) , Chang, Yong Keun (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141 Korea) , Park, Youn-Il (Department of Biological Sciences, Chungnam National University, Daejeon, 34134 Korea)

Abstract ▼ AI-Helper

Microalgal photosynthesis provides energy and carbon-containing precursors for the biosynthesis of storage carbohydrates such as starch, chrysolaminarin, lipids, and cell wall components. Under mild nitrogen deficiency (N−), some Nannochloropsis species accumulate lipid by augmenting cytosolic fatty acid biosynthesis with a temporary increase in laminarin. Accordingly, biosynthesis of the cellulose-rich cell wall should change in response to N− stress because this biosynthetic pathway begins with utilisation of the hexose phosphate pool supplied from photosynthesis. However, few studies have characterised microalgal cell wall metabolism, including oleaginous Nannochloropsis sp. microalgae subjected to nitrogen deficiency. Here, we investigated N-induced changes in cellulose biosynthesis in N. salina. We observed that N− induced cell wall thickening, concurrently increased the transcript levels of genes coding for UDPG pyrophosphorylase and cellulose synthases, and increased cellulose content. Nannochloropsis salina cells with thickened cell wall were more susceptible to mechanical stress such as bead-beating and sonication, implicating cellulose metabolism as a potential target for cost-effective microalgal cell disruption.

참고문헌 (58)

1. Ma Y Wang Z Yu C Yin Y Zhou G Evaluation of the potential of 9 Nannochloropsis strains for biodiesel production Bioresour. Technol. 2014 167 503 509 10.1016/j.biortech.2014.06.047 25013933

상세보기
2. Wen ZY Chen F Heterotrophic production of eicosapentaenoic acid by microalgae Biotechnol. Adv. 2003 21 273 294 10.1016/S0734-9750(03)00051-X 14499126

상세보기
3. Rodolfi L Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor Biotechnol. Bioeng. 2009 102 100 112 10.1002/bit.22033 18683258

상세보기
4. Radakovits R Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana Nat. Commun. 2012 3 686 10.1038/ncomms1688 22353717

상세보기
5. Vieler A Genome, functional gene annotation, and nuclear transformation of the heterokont oleaginous alga Nannochloropsis oceanica CCMP1779 PLoS Genetics. 2012 8 e1003064 10.1371/journal.pgen.1003064 23166516

상세보기
6. Wang Q Genome editing of model microalgae Nannochloropsis spp. by CRISPR/Cas9 Plant J. 2016 88 1071 1081 10.1111/tpj.13307 27538728

상세보기
7. Schwede S Rehman ZU Gerber M Theiss C Span R Effects of thermal pretreatment on anaerobic digestion of Nannochloropsis salina biomass Bioresour. Technol. 2013 143 505 511 10.1016/j.biortech.2013.06.043 23831893

상세보기
8. Scholz MJ Ultrastructure and composition of the Nannochloropsis gaditana cell wall Eukaryot. Cell. 2014 13 1450 1464 10.1128/EC.00183-14 25239976

상세보기
9. Beacham TA Bradley C White DA Bond P Ali ST Lipid productivity and cell wall ultrastructure of six strains of Nannochloropsis : Implications for biofuel production and downstream processing Algal Res. 2014 6 64 69 10.1016/j.algal.2014.09.003

상세보기
10. Van Donk E Lurling M Hessen DO Lokhorst GM Altered cell wall morphology in nutrient deficient phytoplankton and its impact on grazers Limnol. Oceanography. 1997 42 357 364 10.4319/lo.1997.42.2.0357

상세보기
11. Yap BJJ Crawford SA Dagastine RR Scales PJ Martin GJO Nitrogen deprivation of microalgae: effect on cell size, cell wall thickness, cell strength, and resistance to mechanical disruption J. Ind. Microbiol. Biotechnol. 2016 43 1671 1680 10.1007/s10295-016-1848-1 27778140

상세보기
12. Brown MR The amino-acid and sugar composition of 16 species of microalgae used in mariculture J. Exp. Mar. Biol. Ecol. 1991 145 79 99 10.1016/0022-0981(91)90007-J

상세보기
13. Gelin F Resistant biomacromolecules in marine microalgae of the classes eustigmatophyceae and chlorophyceae: geochemical implications Org. Geochem. 1997 26 659 675 10.1016/S0146-6380(97)00035-1

상세보기
14. Converti A Casazza AA Ortiz EY Perego. P Borghi MD Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production Chem. Eng. Process.: Process Intensification 2009 48 1146 1151 10.1016/j.cep.2009.03.006

상세보기
15. Yeesang C Cheirsilp B Effect of nitrogen, salt, and iron content in the growth medium and light intensity on lipid production by microalgae isolated from freshwater sources in Thailand Bioresource Technol. 2011 102 3034 3040 10.1016/j.biortech.2010.10.013

상세보기
16. Simionato D The response of Nannochloropsis gaditana to nitrogen starvation includes de novo biosynthesis of triacylglycerols, a decrease of chloroplast galactolipids, and reorganization of the photosynthetic apparatus Eukaryot. Cell. 2013 12 665 676 10.1128/EC.00363-12 23457191

상세보기
17. Solovchenko A Interactive effects of salinity, high light, and nitrogen starvation on fatty acid and carotenoid profiles in Nannochloropsis oceanica CCALA 804 Eur. J. Lipid Sci. Technol. 2014 116 635 644 10.1002/ejlt.201300456

상세보기
18. Cooksey KE Guckert JB Williams SA Gallis PR Fluorometric determination of the neutral lipid content of microalgal cells using Nile Red J. Microbiol. Methods 1987 6 333 345 10.1016/0167-7012(87)90019-4

상세보기
19. Gusbeth CA Eing C Göttel M Sträßner R Frey W Fluorescence diagnostics for lipid status monitoring of microalgae during cultivation Int. J. Renew Energy Biofuels. 2016 1 12
20. Dong HP Responses of Nannochloropsis oceanica IMET1 to long-term nitrogen starvation and recovery Plant Physiol. 2013 162 1110 1126 10.1104/pp.113.214320 23637339

상세보기
21. Li X A galactoglycerolipid lipase is required for triacylglycerol accumulation and survival following nitrogen deprivation in Chlamydomonas reinhardtii Plant Cell 2012 24 4670 4686 10.1105/tpc.112.105106 23161887

상세보기
22. Corteggiani Carpinelli E Chromosome scale genome assembly and transcriptome profiling of Nannochloropsis gaditana in nitrogen depletion Mol. Plant 2014 7 323 335 10.1093/mp/sst120 23966634

상세보기
23. Li F Gao D Hu H High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product Biosci. Biotechnol. Biochem. 2014 78 812 817 10.1080/09168451.2014.905184 25035984

상세보기
24. Petroutsos D Evolution of galactoglycerolipid biosynthetic pathways: from cyanobacteria to primary plastids and from primary to secondary plastids Prog. Lipid Res. 2014 54 68 85 10.1016/j.plipres.2014.02.001 24594266

상세보기
25. Jia J Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions Algal Res. 2015 7 66 77 10.1016/j.algal.2014.11.005

상세보기
26. Wang D Na nnochloropsis genomes reveal evolution of microalgal oleaginous traits PLoS Genet. 2014 10 e1004094 10.1371/journal.pgen.1004094 24415958

상세보기
27. Domergue F Acyl carriers used as substrates by the desaturases and elongases involved in very long-chain polyunsaturated fatty acids biosynthesis reconstituted in yeast J. Biol. Chem. 2003 278 35115 35126 10.1074/jbc.M305990200 12835316

상세보기
28. Domergue F New insight into Phaeodactylum tricornutum fatty acid metabolism. Cloning and functional characterization of plastidial and microsomal Δ12-fatty acid desaturases Plant Physiol. 2003 131 1648 1660 10.1104/pp.102.018317 12692324

상세보기
29. Michaelson LV Lazarus CM Griffiths G Napier JA Stobart AK Isolation of a ∆5-fatty acid desaturase gene from Mortierella alpina J. Biol. Chem. 1998 273 19055 19059 10.1074/jbc.273.30.19055 9668087

상세보기
30. Peng KT Delta 5 fatty acid desaturase upregulates the synthesis of polyunsaturated fatty acids in the marine didatom Phaeodactylum tricornutum J. Agric. Food Chem. 2014 62 8773 8776 10.1021/jf5031086 25109502

상세보기
31. Hu Q Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances Plant J. 2008 54 621 639 10.1111/j.1365-313X.2008.03492.x 18476868

상세보기
32. Turchetto-Zolet A Evolutionary view of acyl-CoA diacylglycerol acyltransferase (DGAT), a key enzyme in neutral lipid biosynthesis BMC Evol. Bio. 2011 11 263 10.1186/1471-2148-11-263 21933415

상세보기
33. Wang D Nannochloropsis genomes reveal evolution of microalgal oleaginous traits PloS Genet. 2014 10 e1004094 10.1371/journal.pgen.1004094 24415958

상세보기
34. Herth W Schnepf E The fluorochrome, calcofluor white, binds oriented to structural polysaccharide fibrils Protoplasma 1980 105 129 133 10.1007/BF01279855

상세보기
35. Günerken E Cell disruption for microalgae biorefineries Biotechnol. Adv. 2015 33 243 260 10.1016/j.biotechadv.2015.01.008 25656098

상세보기
36. Carpita NC Tensile strength of cell walls of living cells Plant Physiol. 1985 79 485 488 10.1104/pp.79.2.485 16664436

상세보기
37. Lee AK Lewis DM Ashman PJ Disruption of microalgal cells for the extraction of lipids for biofuels: processes and specific energy requirements Biomass Bioener. 2012 46 89 101 10.1016/j.biombioe.2012.06.034

상세보기
38. Zetsche EM Meysman FJR Dead or alive? Viability assessment of micro- and mesoplankton J. Plankton Res. 2012 34 493 509 10.1093/plankt/fbs018

상세보기
39. Hamann T Global expression analysis of CES and CSL genes in Arabidopsis Cellulose 2004 11 279 286 10.1023/B:CELL.0000046340.99925.57

상세보기
40. Xie L Yang C Wang X Brassinosteroids can regulate cellulose biosynthesis by controlling the expression of CES genes in Arabidopsis J. Exp. Bot. 2011 62 4495 4506 10.1093/jxb/err164 21617247

상세보기
41. Li X Two stages of N-deficient cultivation enhance the lipid content of microalga Scenedesmus sp J. Am. Oil Chem. Soc. 2015 92 503 512 10.1007/s11746-015-2613-8

상세보기
42. Petrie JR Recruiting a new substrate for triacylglycerol synthesis in plants: the monoacylglycerol acyltransferase pathway PLoS One 2012 7 e35214 10.1371/journal.pone.0035214 22523576

상세보기
43. Meng Y The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes Bioresour. Technol. 2015 179 483 489 10.1016/j.biortech.2014.12.012 25575208

상세보기
44. Slocombe SP Unlocking nature’s treasure-chest: screening for oleaginous algae Sci. Rep. 2015 5 9844 10.1038/srep09844 26202369

상세보기
45. Sukenik A Carmeli Y Berner T Regulation of fatty acid composition by irradiance level in the eustigmatophyte Nannochloropsis sp J. Phycol. 1989 25 686 692 10.1111/j.0022-3646.1989.00686.x

상세보기
46. Alboresi A Light remodels lipid biosynthesis in Nannochloropsis gaditana by modulating carbon partitioning between organelles Plant Physiol. 2016 171 2468 2482 27325666

상세보기
47. Pal D Khozin-Goldberg I Cohen Z Boussiba S The effect of light, salinity, and nitrogen availability on lipid production by Nannochloropsis sp Appl. Microbiol. Biotechnol. 2011 90 1429 1441 10.1007/s00253-011-3170-1 21431397

상세보기
48. Sforza E Simionato D Giacometti GM Bertucco A Morosinotto T Adjusted light and dark cycles can optimize photosynthetic efficiency in algae growing in photobioreactors PLoS One. 2012 7 e38975 10.1371/journal.pone.0038975 22745696

상세보기
49. Teo CL Idris A Zain NAM Taisir M Synergistic effect of optimizing light-emitting diode illumination quality and intensity to manipulate composition of fatty acid methyl esters from Nannochloropsis sp Bioresour. Technol. 2014 173 284 290 10.1016/j.biortech.2014.09.110 25310864

상세보기
50. Kang NK Effects of overexpression of a bHLH transcription factor on biomass and lipid production in Nannochloropsis salina Biotech. Biofuels 2015 8 200 10.1186/s13068-015-0386-9

상세보기
51. Park JY Sonication-assisted homogenization system for improved lipid extraction from Chlorella vulgaris Renewable Energy 2015 79 3 8 10.1016/j.renene.2014.10.001

상세보기
52. Guillard RR Ryther JH Studies of marine planktonic diatoms. I. Cyclotella nana (Hustedt), and Detonula confervacea (cleve) Gran Can. J. Microbiol. 1962 8 229 239 10.1139/m62-029 13902807

상세보기
53. Moran R Porath D Chlorophyll determination in intact tissues using N, N-dimethylformamide Plant Physiol. 1980 65 478 479 10.1104/pp.65.3.478 16661217

상세보기
54. Porra R Thompson W Kriedemann P Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy Biochim. Biophys. Acta. 1989 975 384 394 10.1016/S0005-2728(89)80347-0

상세보기
55. Reynolds ES The use of lead citrate at high pH as an electron-opaque stain in electron microscopy J. Cell Biol. 1963 17 208 212 10.1083/jcb.17.1.208 13986422

상세보기
56. Hurst PL Sullivan PA Shepherd MG Substrate specificity and mode of action of a cellulase from Aspergillus niger. Biochem. J. 1978 169 389 395 629761
57. Sasser M Identification of bacteria by gas chromatography of cellular fatty acids USFCC Newsletter 1990 20 1 6
58. Lu Y Xie L Chen J A novel procedure for absolute real-time quantification of gene expression patterns Plant Methods 2012 8 9 10.1186/1746-4811-8-9 22404915

상세보기

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Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina 원문보기

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Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina 원문보기

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