[논문]Agerarin, identified from Ageratum houstonianum , stimulates circadian CLOCK-mediated aquaporin-3 gene expression in HaCaT keratinocytes

Shin, Soon Young; Lee, Da Hyun; Gil, Ha-Na; Kim, Beom Soo; Choe, Jeong-Sook; Kim, Jung-Bong; Lee, Young Han; Lim, Yoongho

doi:10.1038/s41598-017-11642-x

[해외논문] Agerarin, identified from Ageratum houstonianum , stimulates circadian CLOCK-mediated aquaporin-3 gene expression in HaCaT keratinocytes 원문보기

Scientific reports, v.7, 2017년, pp.11175 -

Shin, Soon Young (Department of Biological Sciences, Sanghuh College of Life Sciences, Konkuk University, Seoul, 05029 Republic of Korea) , Lee, Da Hyun (Department of Biological Sciences, Sanghuh College of Life Sciences, Konkuk University, Seoul, 05029 Republic of Korea) , Gil, Ha-Na (Cancer and Metabolism Institute, Konkuk University, Seoul, 05029 Republic of Korea) , Kim, Beom Soo (Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul, 05029 Republic of Korea) , Choe, Jeong-Sook (Department of Agrofood Resources, National Academy of Agricultural Sciences, Rural Development Administration, Jeonju, 55365 Republic of Korea) , Kim, Jung-Bong (Department of Agrofood Resources, National Academy of Agricultural Sciences, Rural Development Administration, Jeonju, 55365 Republic of Korea) , Lee, Young Han (Department of Biological Sciences, Sanghuh College of Life Sciences, Konkuk University, Seoul, 05029 Republic of Korea) , Lim, Yoongho (Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul, 05029 Republic of Korea)

Abstract ▼ AI-Helper

The juice of Ageratum houstonianum is used in folk medicine as an external wound healing aid for skin injuries. However, the active component of A. houstonianum and its mode of action in skin wound healing has not been investigated. This study was conducted to investigate the effect of A. houstonianum ethanolnolic extract (AHE) on the expression of aquaporin-3 (AQP3), an integral membrane protein for water and glycerol transport in keratinocytes, and to identify the structure of the A. houstonianum bioactive compound. Here, we show that AHE increased AQP3 gene expression at the transcriptional level through the p38 MAPK pathway in HaCaT cells. Furthermore, AHE ameliorated suppression of AQP3 expression caused by ultraviolet B (UVB) irradiation. Agerarin (6,7-dimethoxy-2,2-dimethyl-2H-chromene) was identified as the bioactive compound responsible for the up-regulation of AQP3 expression by enhancing the expression of the transcription factor circadian locomotor output cycles kaput (CLOCK). In conclusion, agerarin is a bioactive compound in AHE responsible for CLOCK-mediated AQP3 expression in keratinocytes.

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