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[국내논문] Droplet-based digital PCR system for detection of single-cell level of foodborne pathogens

Biochip journal, v.11 no.4, 2017년, pp.329 - 337  

Jang, Minjeong ,  Jeong, Soon Woo ,  Bae, Nam Ho ,  Song, Younseong ,  Lee, Tae Jae ,  Lee, Moon Keun ,  Lee, Seok Jae ,  Lee, Kyoung G.

Abstract AI-Helper 아이콘AI-Helper

Recently, foodborne pathogen is a common and distressing disease around world to cause a threat to life and economic damages and it is urgent to develop a tools to diagnosis of such pathogens in the early stage to prevent potential outbreak. Although conventional cell extraction and recovery of DNA ...

참고문헌 (24)

  1. Emerg. Infect. Dis. E. Scallan 17 7 2011 10.3201/eid1701.P11101 Scallan, E. et al. Foodborne illness acquired in the United States-major pathogens. Emerg. Infect. Dis. 17, 7 (2011). 

  2. Emerg. Infect. Dis. E. Scallan 17 16 2011 10.3201/eid1701.P21101 Scallan, E. et al. Foodborne illness acquired in the United States-unspecified agents. Emerg. Infect. Dis. 17, 16 (2011). 

  3. Nature D.M. Morens 430 242 2004 10.1038/nature02759 Morens, D.M., Folkers, G.K. & Fauci, A.S. The challenge of emerging and re-emerging infectious diseases. Nature 430, 242-249 (2004). 

  4. Molecules R. Khan 14 586 2009 10.3390/molecules14020586 Khan, R. et al. Antimicrobial activity of five herbal extracts against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin. Molecules 14, 586-597 (2009). 

  5. Biotechnol. Adv. V. Velusamy 28 232 2010 10.1016/j.biotechadv.2009.12.004 Velusamy, V. et al. An overview of foodborne pathogen detection: In the perspective of biosensors. Biotechnol. Adv. 28, 232-254 (2010). 

  6. J. Clin. Microbiol. M.P. Weinstein 33 978 1995 10.1128/JCM.33.4.978-981.1995 Weinstein, M.P. et al. Controlled evaluation of BacT/Alert standard aerobic and FAN aerobic blood culture bottles for detection of bacteremia and fungemia. J. Clin. Microbiol. 33, 978-981 (1995). 

  7. J. Infect. S. Gebert 57 307 2008 10.1016/j.jinf.2008.07.013 Gebert, S., Siegel, D. & Wellinghausen, N. Rapid detection of pathogens in blood culture bottles by realtime PCR in conjunction with the pre-analytic tool MolYsis. J. Infect. 57, 307-316 (2008). 

  8. J.M. Rangel 2005 Epidemiology of Escherichia coli O157:H7 outbreaks Rangel, J.M. et al. Epidemiology of Escherichia coli O157:H7 outbreaks, United States, 1982-2002 (2005). 

  9. Can. Med. Assoc. J. M. Louie 163 301 2000 Louie, M., Louie, L. & Simor, A.E. The role of DNA amplification technology in the diagnosis of infectious diseases. Can. Med. Assoc. J. 163, 301-309 (2000). 

  10. Lab Chip Z. Zhu 12 3907 2012 10.1039/c2lc40461c Zhu, Z. et al. Highly sensitive and quantitative detection of rare pathogens through agarose droplet microfluidic emulsion PCR at the single-cell level. Lab Chip 12, 3907-3913 (2012). 

  11. Nat. Methods O.J. Miller 3 561 2006 10.1038/nmeth897 Miller, O.J. et al. Directed evolution by in vitro compartmentalization. Nat. Methods 3, 561-570 (2006). 

  12. Nature Methods C.M. Hindson 10 1003 2013 10.1038/nmeth.2633 Hindson, C.M. et al. Absolute quantification by droplet digital PCR versus analog real-time PCR. Nature Methods 10, 1003-1005 (2013). 

  13. Clin. Chem. J.F. Huggett 61 79 2015 10.1373/clinchem.2014.221366 Huggett, J.F., Cowen, S. & Foy, C.A. Considerations for digital PCR as an accurate molecular diagnostic tool. Clin. Chem. 61, 79-88 (2015). 

  14. Biosens. Bioelectron. L. Cao 90 459 2017 10.1016/j.bios.2016.09.082 Cao, L. et al. Advances in digital polymerase chain reaction (ddPCR) and its emerging biomedical applications. Biosens. Bioelectron. 90, 459-474 (2017). 

  15. Sci. Rep. S.C.-H. Tsao 5 11198 2015 10.1038/srep11198 Tsao, S.C.-H. et al. Monitoring response to therapy in melanoma by quantifying circulating tumour DNA with droplet digital PCR for BRAF and NRAS mutations. Sci. Rep. 5, 11198 (2015). 

  16. Clin. Chem. R.H. Sedlak 60 765 2014 10.1373/clinchem.2013.217240 Sedlak, R.H. et al. Identification of chromosomally integrated human herpesvirus 6 by droplet digital PCR. Clin. Chem. 60, 765-772 (2014). 

  17. Trends Analyt. Chem. S. Mashaghi 82 118 2016 10.1016/j.trac.2016.05.019 Mashaghi, S., Abbaspourrad, A., Weitz, D.A. & van Oijen, A.M. Droplet microfluidics: A tool for biology, chemistry and nanotechnology. Trends Analyt. Chem. 82, 118-125 (2016). 

  18. Biotechnol. Bioeng. E. Cámara 113 1542 2016 10.1002/bit.25916 Cámara, E., Albiol, J. & Ferrer, P. Droplet digital PCRaided screening and characterization of Pichia pastoris multiple gene copy strains. Biotechnol. Bioeng. 113, 1542 (2016). 

  19. A. Rakszewska 2014 One drop at a time: toward droplet microfluidics as a versatile tool for single-cell analysis Rakszewska, A., Tel, J., Chokkalingam, V. & Huck, W.T. One drop at a time: toward droplet microfluidics as a versatile tool for single-cell analysis. NPG Asia Mater. 6, e133 (2014). 

  20. Anal. Chem. T. Geng 86 703 2013 10.1021/ac403137h Geng, T., Novak, R. & Mathies, R.A. Single-cell forensic short tandem repeat typing within microfluidic droplets. Anal. Chem. 86, 703-712 (2013). 

  21. Lab Chip V. Chokkalingam 13 4740 2013 10.1039/c3lc50945a Chokkalingam, V. et al. Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics. Lab Chip 13, 4740-4744 (2013). 

  22. Lab Chip X. Leng 10 2841 2010 10.1039/c0lc00145g Leng, X. et al. Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR. Lab Chip 10, 2841-2843 (2010). 

  23. Forensic Science International: Genetics T. Geng 14 203 2015 10.1016/j.fsigen.2014.10.007 Geng, T. & Mathies, R.A. Minimizing inhibition of PCR-STR typing using digital agarose droplet microfluidics. Forensic Science International: Genetics 14, 203-209 (2015). 

  24. Lab Chip K.J. Park 14 1873 2014 10.1039/c4lc00070f Park, K.J. et al. Micropillar arrays enabling single microbial cell encapsulation in hydrogels. Lab Chip 14, 1873-1879 (2014). 

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