[논문]Highly sensitive platinum nanoparticles-embedded porous graphene sensor for monitoring ROS from living cells upon oxidative stress

Liu, Yidan; Shang, Tianyi; Liu, Yuelin; Liu, Xinhe; Xue, Zhonghua; Liu, Xiuhui

doi:10.1016/j.snb.2018.02.135

Highly sensitive platinum nanoparticles-embedded porous graphene sensor for monitoring ROS from living cells upon oxidative stress

Sensors and actuators. B, Chemical, v.263, 2018년, pp.543 - 549

Liu, Yidan (Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China) , Shang, Tianyi (Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China) , Liu, Yuelin (Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China) , Liu, Xinhe (Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China) , Xue, Zhonghua (Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China) , Liu, Xiuhui (Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Ch)

Abstract ▼ AI-Helper

Abstract Real-time monitoring reactive oxygen species (ROS) released from living cells is important in both physiological and pathological contexts. The excessive amount of ROS accumulation in cells could lead to a series of cell damage and cause aging and disease. Here we present design, fabrication, and successful testing of a highly sensitive electrochemical hydrogen peroxide sensor based on a three-dimensional (3D) platinum nanoparticles (PtNPs) decorated porous graphene composite. Scanning electron microscopy (SEM) investigations indicate that PtNPs were embedded into porous graphene (PGN), something that was rarely reported. With the help of the excellent catalytic activity of PtNPs and unique porous structure in combination with inherent properties of graphene, the PtNPs/PGN based sensor presents efficient electrochemical performance for H2O2 sensing with a detection limit of 0.02 nM, the lowest detection limit achieved in nonenzymatic electrochemical sensors so far, which guarantees the possibility of sensitive and reliable detection of H2O2 release from living cells. This work opens a new avenue for direct preparation of metal nanoparticle-embedded porous graphene composite. More importantly, the presented sensors can be successfully used for monitoring changes of H2O2 levels upon different stimuli and collecting kinetic information on cellular ROS release, which provides more opportunities to study its cellular biochemistry. Highlights Explored a facile way to prepare metal nanoparticle−embedded porous graphene composite films. Constructed a novel non-enzyme analysis platform based on PtNPs/PGN materials. A significant low detection limit of 0.02 nM and wider linear range of 7 orders of magnitude were achieved. The sensor was used for in situ detecting H2O2 from living cells with different oxidative stress. Graphical abstract In this work, PtNPs and porous graphene (PGN) were combined skillfully by a simple one-step carbothermal reaction to fabricate H2O2 sensor. This composite not only presents higher catalytic ability for H2O2, but also improves mass transfer efficiency, enlarges specific surface area and increases the active sites. Due to the PtNPs/PGN modified glassy carbon electrode exhibited excellent electrochemical performance, it could be applied to determination of H2O2 released from living cells. [DISPLAY OMISSION]

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참고문헌 (29)

Nat. Rev. Mol. Cell Biol. Giorgio 8 722 2007 10.1038/nrm2240 Hydrogen peroxide: a metabolic by-product or a common mediator of ageing signals

상세보기
Biosens. Bioelectron. Liu 90 39 2017 10.1016/j.bios.2016.11.015 Construction of a highly sensitive non-enzymatic sensor for superoxide anion radical detection from living cells

상세보기
Cell Rossi 132 681 2008 10.1016/j.cell.2008.01.036 Stems cells and the pathways to aging and cancer

상세보기
J. Am. Chem. Soc. de Gracia Lux 134 15758 2012 10.1021/ja303372u Biocompatible polymeric nanoparticles degrade and release cargo in response to biologically relevant levels of hydrogen peroxide

상세보기
Anal. Chem. Xu 87 1968 2015 10.1021/ac5043895 Unraveling the role of hydrogen peroxide in α-synuclein aggregation using an ultrasensitive nanoplasmonic probe

상세보기
Biosens. Bioelectron. Rahimi 25 1301 2010 10.1016/j.bios.2009.10.020 Ionic-liquid/NH2-MWCNTs as a highly sensitive nano-composite for catalase direct electrochemistry

상세보기
Anal. Chim. Acta Matos 441 73 2001 10.1016/S0003-2670(01)01094-7 Flow-injection system with enzyme reactor for differential amperometric determination of hydrogen peroxide in rainwater

상세보기
Biosens. Bioelectron. Cao 35 101 2012 10.1016/j.bios.2012.02.027 Bioactivity of horseradish peroxidase entrapped in silica nanospheres

상세보기
Biosens. Bioelectron. Liu 87 101 2017 10.1016/j.bios.2016.08.015 Horseradish peroxidase supported on porous graphene as a novel sensing platform for detection of hydrogen peroxide in living cells sensitively

상세보기
Talanta Lin 82 340 2010 10.1016/j.talanta.2010.04.047 Electrode modified with a composite film of ZnO nanorods and Ag nanoparticles as a sensor for hydrogen peroxide

상세보기
Electrochim. Acta Fang 70 266 2012 10.1016/j.electacta.2012.03.105 A non-enzymatic hydrogen peroxide sensor based on poly (vinyl alcohol)-multiwalled carbon nanotubes-platinum nanoparticles hybrids modified glassy carbon electrode

상세보기
Funct. Mater. Lett. Wang 9 1650051 2016 10.1142/S179360471650051X Graphite-specific peptide mediated synthesis of Pt nanoparticles on reduced graphene oxide for electrochemical detection of H2O2

상세보기
Carbohydr. Polym. Karthik 164 102 2017 10.1016/j.carbpol.2017.01.077 Rapid synthesis of ethyl cellulose supported platinum nanoparticles for the non-enzymatic determination of H2O2

상세보기
Anal. Methods Lu 8 816 2016 10.1039/C5AY02095F Green and rapid synthesis of a water-dispersible Pt-reduced graphene oxide hybrid material for hydrogen peroxide detection

상세보기
Anal. Chem. Zhang 86 9459 2014 10.1021/ac5009699 Highly sensitive graphene-Pt nanocomposites amperometric biosensor and its application in living cell H2O2 detection

상세보기
Biosens. Bioelectron. Liu 74 71 2015 10.1016/j.bios.2015.06.042 Highly exposed Pt nanoparticles supported on porous graphene for electrochemical detection of hydrogen peroxide in living cells

상세보기
Langmuir Xi 28 9885 2012 10.1021/la301440k Gold nanoparticle-embedded porous graphene thin films fabricated via layer-by-layer self-assembly and subsequent thermal annealing for electrochemical sensing

상세보기
ACS Nano Guo 4 3959 2010 10.1021/nn100852h Platinum nanoparticle ensemble-on-graphene hybrid nanosheet: one-pot, rapid synthesis, and used as new electrode material for electrochemical sensing

상세보기
Electrochem. Commun. Li 11 846 2009 10.1016/j.elecom.2009.02.009 Preparation and electrochemical performance for methanol oxidation of Pt/graphene nanocomposites

상세보기
Nat. Nanotechnol. Li 3 101 2008 10.1038/nnano.2007.451 Processable aqueous dispersions of graphene nanosheets

상세보기
Carbon Cao 89 41 2015 10.1016/j.carbon.2015.03.003 Metal etching method for preparing porous graphene as high performance anode material for lithium-ion batteries

상세보기
Appl. Surf. Sci. Hsieh 277 223 2013 10.1016/j.apsusc.2013.04.029 Study of Pt catalyst on graphene and its application to fuel cell

상세보기
Fu 359 2006 Physical Chemistry
Anal. Biochem. Liao 402 47 2010 10.1016/j.ab.2010.03.015 Study on an amperometric immunosensor based on nafion-cysteine composite membrane for detection of carcinoembryonic antigen

상세보기
Angew. Chem. Int. Ed. Wolfbeis 41 4495 2002 10.1002/1521-3773(20021202)41:23<4495::AID-ANIE4495>3.0.CO;2-I A europium-ion-based luminescent sensing probe for hydrogen peroxide

상세보기
Biosens. Bioelectron. Zhu 80 601 2016 10.1016/j.bios.2016.02.019 PtW/MoS2 hybrid nanocomposite for electrochemical sensing of H2O2 released from living cells

상세보기
Anal. Chem. Wang 85 10289 2013 10.1021/ac402114c Biosensor based on ultrasmall MoS2 nanoparticles for electrochemical detection of H2O2 released by cells at the nanomolar level

상세보기
ACS Appl. Mater. Interfaces Maji 6 13648 2014 10.1021/am503110s Immobilizing gold nanoparticles in mesoporous silica covered reduced graphene oxide: a hybrid material for cancer cell detection through hydrogen peroxide sensing

상세보기
Proc. Natl. Acad. Sci. Liu 108 2668 2011 10.1073/pnas.1018391108 Real-time mapping of a hydrogen peroxide concentration profile across a polymicrobial bacterial biofilm using scanning electrochemical microscopy

상세보기

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Highly sensitive platinum nanoparticles-embedded porous graphene sensor for monitoring ROS from living cells upon oxidative stress

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