[논문]Application of a phage in decontaminating Vibrio parahaemolyticus in oysters

Zhang, Hui; Yang, Zhenquan; Zhou, Yan; Bao, Hongduo; Wang, Ran; Li, Tingwu; Pang, Maoda; Sun, Lichang; Zhou, Xiaohui

doi:10.1016/j.ijfoodmicro.2018.03.027

Application of a phage in decontaminating Vibrio parahaemolyticus in oysters

International journal of food microbiology, v.275, 2018년, pp.24 - 31

Zhang, Hui (Jiangsu Key Laboratory of Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) , Yang, Zhenquan (College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225001, China) , Zhou, Yan (Jiangsu Key Laboratory of Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) , Bao, Hongduo (Jiangsu Key Laboratory of Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) , Wang, Ran (Jiangsu Key Laboratory of Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) , Li, Tingwu (Jiangsu Key Laboratory of Food Quality and Safety-State Key Laboratory Cultivation Base of MOST, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) , Pang, Maoda (Jiangsu Key Laboratory of Food Quality and Safety-State Key Laboratory Cultivation Base o) , Sun, Lichang , Zhou, Xiaohui

Abstract ▼ AI-Helper

Abstract Vibrio parahaemolyticus is a major pathogen that is mainly associated with seafood and is a global concern of food safety. With high prevalence of contamination in food, efficient strategy is needed to decontaminate those contaminated foods and control the emergence of vibriosis. In the present study, a V. parahaemolyticus-specific phage vB_VpaS_OMN (designated as phage OMN) was isolated from oyster. Phage OMN had good pH (5–9) and temperature tolerance (<50 °C). Phage OMN exhibited broad host range against isolates of V. parahaemolyticus (20/31). After treatment with phage OMN in the liquid condition for 7 h, the number of V. parahaemolyticus was reduced significantly compared to control treatment. When phage OMN was applied to oyster samples for 48 and 72 h, 90% and 99%, respectively, of V. parahaemolyticus was inactivated on Oyster meat surface. Sequence analysis showed that phage OMN had a 42.202 bp genome and revealed about 59.04% homology with Cronobacter phage vB_CsaP_Ss1. Only 10 CDSs can be predicted based on the GenBank database, while 42% of the CDSs were unique to OMN and had no known function, indicating that phage OMN is a new lytic phage. Fully understanding of the function for the phage genes and the properties of the phage is important for the development of strategies to control V. parahaemolyticus contamination in oysters and disease in aquaculture. Highlights We isolated a new phage designated as Vibrio parahaemolyticus phage OMN. The whole genome of phage OMN was sequenced. The genome of phage OMN has low homology with known phage genes. Phage OMN exhibited broad host range against isolates of V. parahaemolyticus. When phage OMN was applied to oyster samples, 99% of V. parahaemolyticus can be inactivated on Oyster meat surface.

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Application of a phage in decontaminating Vibrio parahaemolyticus in oysters

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