[논문]Investigation of constituents from Cinnamomum camphora (L.) J. Presl and evaluation of their anti-inflammatory properties in lipopolysaccharide-stimulated RAW 264.7 macrophages

Li, Yan-Ru; Fu, Chun-Sheng; Yang, Wen-Jing; Wang, Xiao-Ling; Feng, Dan; Wang, Xiao-Ning; Ren, Dong-Mei; Lou, Hong-Xiang; Shen, Tao

doi:10.1016/j.jep.2018.04.017

[해외논문] Investigation of constituents from Cinnamomum camphora (L.) J. Presl and evaluation of their anti-inflammatory properties in lipopolysaccharide-stimulated RAW 264.7 macrophages

Journal of ethnopharmacology, v.221, 2018년, pp.37 - 47

Li, Yan-Ru (Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, People's Republic of China) , Fu, Chun-Sheng (School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan, People's Republic of China) , Yang, Wen-Jing (Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, People's Republic of China) , Wang, Xiao-Ling (The Second Hospital of Shandong University, 247 Bei-Yuan Street, Jinan, People's Republic of China) , Feng, Dan (Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, People's Republic of China) , Wang, Xiao-Ning (Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, People's Republic of China) , Ren, Dong-Mei (Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, People's Republic of China) , Lou, Hong-Xiang (Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, People's Republic of China) , Shen, Tao (Key Lab of Ch)

Abstract ▼ AI-Helper

Abstract Ethnopharmacology relevance Cinnamomum camphora (L.) J. Presl has been used for the traditional medicine as a therapeutic agent of inflammation-related diseases, including sprains, rheumatic arthritis, abdominal pain, cough and bronchitis, for a long history. The aim of the present study was to illustrate anti-inflammatory substances of C. camphora and their mechanism of action, and to establish the correlations between chemical constituents and traditional uses of this plant. Materials and methods Chemical constituents were purified by chromatographic methods, and their structures were established based on spectroscopic analysis. Lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages was adopted for evaluating the anti-inflammatory activity in vitro. The nitric oxide (NO) production assay and nuclear factor kappa B (NF-κB) dual luciferase reporter assay were used to screen anti-inflammatory constituents. The mRNA and protein levels of inflammation-related cytokines and enzymes were determined by real-time reverse transcription-polymerase chain reaction (RT-PCR), immunoblot analysis, and enzyme linked immunosorbent assay (ELISA), respectively. Results Twenty-five constituents were isolated from the EtOH extract of C. camphora. Eight constituents, covering phenylpropanoid (7), lignans (10 and 22), flavonoids (16–18), coumarin (21), and terpenoid (24) significantly inhibited LPS-stimulated NO production with maximum inhibition rates (MIRs) of ≥ 80%, and thus were verified to be the anti-inflammatory substances of this ethnomedical plant. (+)-Episesaminone (SMO, 22) and 3S-(+)−9-oxonerolidol (NLD, 24) blocked NF-κB activation via inducing IκBα expression. Moreover, SMO and NLD inhibited productions of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and prostaglandin E2 (PGE2), and alleviated increased mRNA and protein levels of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and matrix metallopeptidase-9 (MMP-9) in LPS-stimulated RAW 264.7 macrophages. Conclusions The ethnomedical use of C. camphora for the treatment of inflammation-related diseases was attributed to the combined in vitro anti-inflammatory activities of phenylpropanoid, lignan, flavonoid, coumarin, and terpenoid. SMO and NLD were found to be new molecules with in vitro anti-inflammatory activities, which are achieved by inhibiting NF-κB regulated inflammatory response. Graphical abstract [DISPLAY OMISSION]

Keyword

참고문헌 (48)

Life Sci. Aktan 75 639 2004 10.1016/j.lfs.2003.10.042 iNOS-mediated nitric oxide production and its regulation

상세보기
J. Immunol. Blackwell 157 1630 1996 10.4049/jimmunol.157.4.1630 In vivo antioxidant treatment suppresses nuclear factor-kappa B activation and neutrophilic lung inflammation

상세보기
Fitoterapia Cabral 71 1 2000 10.1016/S0367-326X(99)00105-7 Effects of some lignans and neolignans on the development and excretion of Rhodnius prolixus

상세보기
J. Agric. Food Chem. Carpinella 53 2922 2005 10.1021/jf0482461 Antifungal synergistic effect of scopoletin, a hydroxycoumarin isolated from Melia azedarach L. fruits

상세보기
J. Ethnopharmacol. Chen 170 72 2015 10.1016/j.jep.2015.05.004 The anti-inflammatory activities of Ainsliaea fragrans Champ. extract and its components in lipopolysaccharide-stimulated RAW264.7 macrophages through inhibition of NF-kappa B pathway

상세보기
Braz. Arch. Biol. Technol. Frizzo 43 313 2000 10.1590/S1516-89132000000300011 Essential oils of camphor tree (Cinnamomum camphora Nees & Eberm) cultivated in Southern Brazil

상세보기
J. Food Sci. Fu 80 H1912 2015 10.1111/1750-3841.12943 Camphor tree seed kernel oil reduces body fat deposition and improves blood lipids in rats

상세보기
Inflamm. Res. Garcia-Lafuente 58 537 2009 10.1007/s00011-009-0037-3 Flavonoids as anti-inflammatory agents: implications in cancer and cardiovascular disease

상세보기
Phytochemistry Garcia 55 611 2000 10.1016/S0031-9422(00)00228-4 Effects of lignoids on a hematophagous bug, Rhodnius prolixus: feeding, ecdysis and diuresis

상세보기
Food Chem. Toxicol. Gasparrini 102 1 2017 10.1016/j.fct.2017.01.018 Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages

상세보기
Ther. Adv. Chronic Dis. George 7 34 2016 10.1177/2040622315609251 Eosinophilic airway inflammation: role in asthma and chronic obstructive pulmonary disease

상세보기
Pharmacol. Ther. Halade 139 32 2013 10.1016/j.pharmthera.2013.03.009 Matrix metalloproteinase (MMP)-9: a proximal biomarker for cardiac remodeling and a distal biomarker for inflammation

상세보기
J. Ethnopharmacol. Han 174 387 2015 10.1016/j.jep.2015.08.044 Comparative evaluation of different cultivars of Flos Chrysanthemi by an anti-inflammatory-based NF-kappa B reporter gene assay coupled to UPLC-Q/TOF MS with PCA and ANN

상세보기
Bull. Chem. Soc. Jpn. Hayashi 42 3591 1969 10.1246/bcsj.42.3591 Two phenol ethers and an aliphatic ketone of the essential oil of the Kusunoki (Cinnamomum camphora Sieb.)

상세보기
Immunobiology Hong 220 1 2015 10.1016/j.imbio.2014.10.004 Anti-inflammatory activities of Physalis alkekengi var. franchetii extract through the inhibition of MMP-9 and AP-1 activation

상세보기
Nat. Prod. Commun. Hsieh 1 21 2006 Lignans from the stem of Cinnamomum camphora
Phytochemistry Iida 21 673 1982 10.1016/0031-9422(82)83163-4 Hydroperoxysesquiterpene and lignan constituents of Magnolia kobus

상세보기
Clin. Exp. Rheumatol. Jarvinen 26 275 2008 Selective iNOS inhibitor 1400W enhances anti-catabolic IL-10 and reduces destructive MMP-10 in OA cartilage. Survey of the effects of 1400W on inflammatory mediators produced by OA cartilage as detected by protein antibody array
J. Pharmacol. Sci. Kim 96 229 2004 10.1254/jphs.CRJ04003X Anti-inflammatory plant flavonoids and cellular action mechanisms

상세보기
J. Ethnopharmacol. Lee 103 208 2006 10.1016/j.jep.2005.08.009 In vitro anti-inflammatory and anti-oxidative effects of Cinnamomum camphora extracts

상세보기
Expert Opin. Ther. Targets Leonidou 2018 10.1080/14728222.2018.1448062 Inducible nitric oxide synthase as a target for osteoarthritis treatment

상세보기
Biomed. Pharmacother. Lind 93 370 2017 10.1016/j.biopha.2017.06.036 Inducible nitric oxide synthase: good or bad?

상세보기
J. Ethnopharmacol. Lucina Torres-Rodriguez 188 266 2016 10.1016/j.jep.2016.04.057 Anti-inflammatory and anti-oxidant effect of Calea urticifolia lyophilized aqueous extract on lipopolysaccharide-stimulated RAW 264.7 macrophages

상세보기
Biochim. Biophys. Acta Ling 1252 15 1995 10.1016/0167-4838(95)00052-V Simultaneous existence of two types of ribosome-inactivating proteins in the seeds of Cinnamonum camphora-characterization of the enzymatic activities of these cytotoxic proteins

상세보기
J. Nat. Prod. Marchand 60 1189 1997 10.1021/np9702953 (+)-episesaminone, a Sesamum indicum furofuran lignan. Isolation and hemisynthesis

상세보기
FASEB J. Marques-Rocha 29 3595 2015 10.1096/fj.14-260323 Noncoding RNAs, cytokines, and inflammation-related diseases

상세보기
PLoS One Miguel 2 e788 2007 10.1371/journal.pone.0000788 A dimer of the Toll-like receptor 4 cytoplasmic domain provides a specific scaffold for the recruitment of signalling adaptor proteins

상세보기
J. Neuropathol. Exp. Neurol. Minghetti 63 901 2004 10.1093/jnen/63.9.901 Cyclooxygenase-2 (COX-2) in inflammatory and degenerative brain diseases

상세보기
Cell Res. Morgan 21 103 2011 10.1038/cr.2010.178 Crosstalk of reactive oxygen species and NF-kappa B signaling

상세보기
J. Clin. Investig. Mulay 123 236 2013 10.1172/JCI63679 Calcium oxalate crystals induce renal inflammation by NLRP3-mediated IL-1 beta secretion

상세보기
Semin. Immunopathol. Nakanishi 35 123 2013 10.1007/s00281-012-0342-8 Multifaceted roles of PGE(2) in inflammation and cancer

상세보기
Turk. J. Pharm. Sci. Nejad 14 201 2017 10.4274/tjps.62207 Pharmacological and toxicological properties of eugenol

상세보기
J. Ethnopharmacol. Park 170 210 2015 10.1016/j.jep.2015.04.012 Aqueous extract of Orostachys japonicus A. Berger exerts immunostimulatory activity in RAW 264.7 macrophages

상세보기
Curr. Obes. Rep. Pereira 3 422 2014 10.1007/s13679-014-0124-9 Low-grade inflammation, obesity, and diabetes

상세보기
Ind. Crop. Prod. Pragadheesh 49 628 2013 10.1016/j.indcrop.2013.06.023 Chemical characterization and antifungal activity of Cinnamomum camphora essential oil

상세보기
Semin. Cancer Biol. Qian 47 170 2017 10.1016/j.semcancer.2017.08.006 Inflammation fires up cancer metastasis

상세보기
Inflamm. Allergy Drug Targets Rathee 8 229 2009 10.2174/187152809788681029 Mechanism of action of flavonoids as anti-inflammatory agents: a review

상세보기
Int. J. Biochem. Cell Biol. Rigoglou 45 2580 2013 10.1016/j.biocel.2013.08.018 The NF-kappa B signalling pathway in osteoarthritis

상세보기
BMC Genom. Shi 17 26 2016 10.1186/s12864-015-2357-8 De novo comparative transcriptome analysis provides new insights into sucrose induced somatic embryogenesis in camphor tree (Cinnamomum camphora L.)

상세보기
Immunol Stephenson 2018 10.1111/imm.12922 Inflammation in CNS neurodegenerative diseases

상세보기
Phytochemistry Suga 33 1395 1993 10.1016/0031-9422(93)85098-C Endogenous Pine Wood Nematocidal Substances in Pines, Pinus-Massoniana, Pinus-Strobus and P-Palustris

상세보기
Chem. Eng. Sun 7 4 2006 Study on the extraction method of flavonoids in Cinnamomum camphora leaves and antioxidation property
J. Clin. Investig. Tak 107 7 2015 10.1172/JCI11830 NF-kappa B: a key role in inflammatory diseases

상세보기
Bull. Chem. Soc. Jpn. Takaoka 50 2821 1977 10.1246/bcsj.50.2821 Studies of lignoids in Lauraceae. III. A new lignan from the heart wood of Cinnamomum camphora Sieb

상세보기
J. Leukoc. Biol. Van Lint 82 1375 2007 10.1189/jlb.0607338 Chemokine and cytokine processing by matrix metalloproteinases and its effect on leukocyte migration and inflammation

상세보기
J. Clin. Investig. Yamamoto 107 135 2001 10.1172/JCI11914 Therapeutic potential of inhibition of the NF-kappa B pathway in the treatment of inflammation and cancer

상세보기
Inflamm. Res. Zelova 62 641 2013 10.1007/s00011-013-0633-0 TNF-alpha signalling and inflammation: interactions between old acquaintances

상세보기
Redox Biol. Zhou 14 154 2018 10.1016/j.redox.2017.09.004 Identification of novel Nrf2 activators from Cinnamomum chartophyllum H. W. Li and their potential application of preventing oxidative insults in human lung epithelial cells

상세보기

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증