[논문]Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells

Lee, Yong Seung; Jung, Woon Yong; Heo, Hyejung; Park, Min Geun; Oh, Seung-Hun; Park, Byong-Gon; Kim, Soonhag

doi:10.1021/acsnano.7b08297

[해외논문] Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells

ACS nano, v.12 no.3, 2018년, pp.2531 - 2538

Lee, Yong Seung (Institute for Bio-Medical Convergence, College of Medicine , Catholic Kwandong University , Gangneung-si , Gangwon-do 270-701 , Republic of Korea) , Jung, Woon Yong (Department of Pathology , Catholic Kwandong University International St. Mary’s Hospital , Incheon Metropolitan City 404-834 , Republic of Korea) , Heo, Hyejung (Institute for Bio-Medical Convergence, College of Medicine , Catholic Kwandong University , Gangneung-si , Gangwon-do 270-701 , Republic of Korea) , Park, Min Geun (Department of Surgery , Catholic Kwandong University International St. Mary’s Hospital , Incheon Metropolitan City 404-834 , Republic of Korea) , Oh, Seung-Hun (Department of Neurology, CHA Bundang Medical Center , CHA University , Seongnam 13497 , Republic of Korea) , Park, Byong-Gon (Department of Physiology, College of Medicine , Catholic Kwandong University , Gangneung-si , Gangwon-do 270-7) , Kim, Soonhag

Abstract ▼ AI-Helper

Exosomes, naturally secreted nanoparticles, have been introduced as vehicles for horizontal transfer of genetic material. We induced autologous exosomes containing a cocktail of reprogramming factors (“reprosomes”) to convert fibroblasts into neural progenitor cells (NPCs). The fibroblasts were treated with ultrasound and subsequently cultured in neural stem cell medium for 1 day to induce the release of reprosomes composed of reprogramming factors associated with chromatin remodeling and neural lineage-specific factors. After being treated with reprosomes, fibroblasts were converted into NPCs (rNPCs) with great efficiency via activation of chromatin remodeling, so quickly that only 5 days were required for the formation of 1500 spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal and proliferative properties for several weeks and successfully differentiated into neurons, astrocytes, and oligodendrocytes in vitro and in vivo. Reprosome-mediated cellular reprogramming is simple, safe, and efficient to produce autologous stem cells for clinical application.[FIG OMISSION]

Keyword

참고문헌 (38)

Li, X., Zuo, X., Jing, J., Ma, Y., Wang, J., Liu, D., Zhu, J., Du, X., Xiong, L., Du, Y., Xu, J., Xiao, X., Wang, J., Chai, Z., Zhao, Y., Deng, H.. Small-Molecule-Driven Direct Reprogramming of Mouse Fibroblasts into Functional Neurons. Cell stem cell, vol.17, no.2, 195-203.

상세보기
Mertens, Jerome, Marchetto, Maria C., Bardy, Cedric, Gage, Fred H.. Evaluating cell reprogramming, differentiation and conversion technologies in neuroscience. Nature reviews. Neuroscience, vol.17, no.7, 424-437.

상세보기
Yu, K.R., Shin, J.H., Kim, J.J., Koog, M., Lee, J., Choi, S., Kim, H.S., Seo, Y., Lee, S., Shin, T.h., Jee, M., Kim, D.W., Jung, S., Shin, S., Han, D., Kang, K.S.. Rapid and Efficient Direct Conversion of Human Adult Somatic Cells into Neural Stem Cells by HMGA2/let-7b. Cell reports, vol.10, no.3, 441-452.

상세보기
Generation of Integration-free and Region-Specific Neural Progenitors from Primate Fibroblasts. Cell reports, vol.3, no.5, 1580-1591.

상세보기
Ring, Karen L., Tong, Leslie M., Balestra, Maureen E., Javier, Robyn, Andrews-Zwilling, Yaisa, Li, Gang, Walker, David, Zhang, William R., Kreitzer, Anatol C., Huang, Yadong. Direct Reprogramming of Mouse and Human Fibroblasts into Multipotent Neural Stem Cells with a Single Factor. Cell stem cell, vol.11, no.1, 100-109.

상세보기
Mirakhori, Fahimeh, Zeynali, Bahman, Rassouli, Hassan, Shahbazi, Ebrahim, Hashemizadeh, Shiva, Kiani, Sahar, Salekdeh, Ghasem Hosseini, Baharvand, Hossein. Induction of Neural Progenitor-Like Cells from Human Fibroblasts via a Genetic Material-Free Approach. PloS one, vol.10, no.8, e0135479-.

상세보기
Joo, Jin Young, Lee, Jonghwan, Ko, Hae Young, Lee, Yong Seung, Lim, Do-Hwan, Kim, Eun-Young, Cho, Sujeong, Hong, Ki-Sung, Ko, Jung Jae, Lee, Suman, Lee, Young Sik, Choi, Youngsok, Lee, Kyung-Ah, Kim, Soonhag. Microinjection free delivery of miRNA inhibitor into zygotes. Scientific reports, vol.4, 5417-.

상세보기
Masuda, Shigeo, Wu, Jun, Hishida, Tomoaki, Pandian, Ganesh N., Sugiyama, Hiroshi, Izpisua Belmonte, Juan Carlos. Chemically induced pluripotent stem cells (CiPSCs): a transgene-free approach. Journal of molecular cell biology : JMCB, vol.5, no.5, 354-355.

상세보기
Hannafon, Bethany N., Ding, Wei-Qun. Intercellular Communication by Exosome-Derived microRNAs in Cancer. International journal of molecular sciences, vol.14, no.7, 14240-14269.

상세보기
Quesenberry, Peter J., Aliotta, Jason, Deregibus, Maria Chiara, Camussi, Giovanni. Role of extracellular RNA-carrying vesicles in cell differentiation and reprogramming. Stem cell research & therapy, vol.6, 153-.

상세보기
Ratajczak, J, Miekus, K, Kucia, M, Zhang, J, Reca, R, Dvorak, P, Ratajczak, M Z. Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery. Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, vol.20, no.5, 847-856.

상세보기
Aliotta, Jason M., Sanchez-Guijo, Fermin M., Dooner, Gerri J., Johnson, Kevin W., Dooner, Mark S., Greer, Kenneth A., Greer, Deborah, Pimentel, Jeffrey, Kolankiewicz, Luiz M., Puente, Napoleon, Faradyan, Sam, Ferland, Paulette, Bearer, Elaine L., Passero, Michael A., Adedi, Mehrdad, Colvin, Gerald A., Quesenberry, Peter J.. Alteration of Marrow Cell Gene Expression, Protein Production, and Engraftment into Lung by Lung-Derived Microvesicles: A Novel Mechanism for Phenotype Modulation. Stem cells^®, vol.25, no.9, 2245-2256.

상세보기
Deregibus, Maria Chiara, Cantaluppi, Vincenzo, Calogero, Raffaele, Lo Iacono, Marco, Tetta, Ciro, Biancone, Luigi, Bruno, Stefania, Bussolati, Benedetta, Camussi, Giovanni. Endothelial progenitor cell-derived microvesicles activate an angiogenic program in endothelial cells by a horizontal transfer of mRNA. Blood, vol.110, no.7, 2440-2448.

상세보기
Eldh, Maria, Ekström, Karin, Valadi, Hadi, Sjöstrand, Margareta, Olsson, Bob, Jernås, Margareta, Lötvall, Jan, Tailleux, Ludovic. Exosomes Communicate Protective Messages during Oxidative Stress; Possible Role of Exosomal Shuttle RNA. PloS one, vol.5, no.12, e15353-.

상세보기
King, Hamish W, Michael, Michael Z, Gleadle, Jonathan M. Hypoxic enhancement of exosome release by breast cancer cells. BMC cancer, vol.12, 421-421.

상세보기
Takeuchi, Toshihide, Suzuki, Mari, Fujikake, Nobuhiro, Popiel, H. Akiko, Kikuchi, Hisae, Futaki, Shiroh, Wada, Keiji, Nagai, Yoshitaka. Intercellular chaperone transmission via exosomes contributes to maintenance of protein homeostasis at the organismal level. Proceedings of the National Academy of Sciences of the United States of America, vol.112, no.19, E2497-E2506.

상세보기
Park, J.A., Sharif, A.S., Tschumperlin, D.J., Lau, L., Limbrey, R., Howarth, P., Drazen, J.M.. Tissue factor-bearing exosome secretion from human mechanically stimulated bronchial epithelial cells in vitro and in vivo. The Journal of allergy and clinical immunology : official organ of American Academy of Allergy, vol.130, no.6, 1375-1383.

상세보기
10.4172/2324-8769.1000105
Brami-Cherrier, Karen, Roze, Emmanuel, Girault, Jean-Antoine, Betuing, Sandrine, Caboche, Jocelyne. Role of the ERK/MSK1 signalling pathway in chromatin remodelling and brain responses to drugs of abuse. Journal of neurochemistry, vol.108, no.6, 1323-1335.

상세보기
Sukezane, Taiko, Oneyama, Chitose, Kakumoto, Kyoko, Shibutani, Kana, Hanafusa, Hidesaburo, Akagi, Tsuyoshi. Human diploid fibroblasts are resistant to MEK/ERK-mediated disruption of the actin cytoskeleton and invasiveness stimulated by Ras. Oncogene, vol.24, no.36, 5648-5655.

상세보기
Cuenda, A., Rousseau, S.. p38 MAP-Kinases pathway regulation, function and role in human diseases. Biochimica et biophysica acta, Molecular cell research, vol.1773, no.8, 1358-1375.

상세보기
Hinde, Elizabeth, Cardarelli, Francesco, Chen, Aaron, Khine, Michelle, Gratton, Enrico. Tracking the mechanical dynamics of human embryonic stem cell chromatin. Epigenetics & chromatin, vol.5, 20-20.

상세보기
Musselman, Catherine A, Lalonde, Marie-Eve, C척t챕, Jacques, Kutateladze, Tatiana G. Perceiving the epigenetic landscape through histone readers. Nature structural & molecular biology, vol.19, no.12, 1218-1227.

상세보기
Reik, Wolf. Stability and flexibility of epigenetic gene regulation in mammalian development. Nature, vol.447, no.7143, 425-432.

상세보기
Kang, P.J., Moon, J.H., Yoon, B.S., Hyeon, S., Jun, E.K., Park, G., Yun, W., Park, J., Park, M., Kim, A., Whang, K.Y., Koh, G.Y., Oh, S., You, S.. Reprogramming of mouse somatic cells into pluripotent stem-like cells using a combination of small molecules. Biomaterials, vol.35, no.26, 7336-7345.

상세보기
Yoshikawa, K., Naitoh, M., Kubota, H., Ishiko, T., Aya, R., Yamawaki, S., Suzuki, S.. Multipotent stem cells are effectively collected from adult human cheek skin. Biochemical and biophysical research communications, vol.431, no.1, 104-110.

상세보기
Vacher, Helene, Mohapatra, Durga P., Trimmer, James S.. Localization and Targeting of Voltage-Dependent Ion Channels in Mammalian Central Neurons. Physiological reviews, vol.88, no.4, 1407-1447.

상세보기
Victor, Matheus B., Richner, M., Hermanstyne, Tracey O., Ransdell, Joseph L., Sobieski, C., Deng, P.Y., Klyachko, Vitaly A., Nerbonne, Jeanne M., Yoo, Andrew S.. Generation of Human Striatal Neurons by MicroRNA-Dependent Direct Conversion of Fibroblasts. Neuron, vol.84, no.2, 311-323.

상세보기
Zhou, Ying, Xu, Huitao, Xu, Wenrong, Wang, Bingying, Wu, Huiyi, Tao, Yang, Zhang, Bin, Wang, Mei, Mao, Fei, Yan, Yongmin, Gao, Shuo, Gu, Hongbing, Zhu, Wei, Qian, Hui. Exosomes released by human umbilical cord mesenchymal stem cells protect against cisplatin-induced renal oxidative stress and apoptosis in vivo and in vitro. Stem cell research & therapy, vol.4, no.2, 34-34.

상세보기
Han, D., Tapia, N., Hermann, A., Hemmer, K., Hoing, S., Arauzo-Bravo, Marcos J., Zaehres, H., Wu, G., Frank, S., Moritz, S., Greber, B., Yang, J., Lee, H., Schwamborn, Jens C., Storch, A., Scholer, Hans R.. Direct Reprogramming of Fibroblasts into Neural Stem Cells by Defined Factors. Cell stem cell, vol.10, no.4, 465-472.

상세보기
Boissart, Claire, Nissan, Xavier, Giraud-Triboult, Karine, Peschanski, Marc, Benchoua, Alexandra. miR-125 potentiates early neural specification of human embryonic stem cells. Development, vol.139, no.7, 1247-1257.

상세보기
Franzoni, Eleonora, Booker, Sam A, Parthasarathy, Srinivas, Rehfeld, Frederick, Grosser, Sabine, Srivatsa, Swathi, Fuchs, Heiko R, Tarabykin, Victor, Vida, Imre, Wulczyn, F Gregory. miR-128 regulates neuronal migration, outgrowth and intrinsic excitability via the intellectual disability gene Phf6. eLife, vol.4, e04263-.

상세보기
Victor, Matheus B., Richner, M., Hermanstyne, Tracey O., Ransdell, Joseph L., Sobieski, C., Deng, P.Y., Klyachko, Vitaly A., Nerbonne, Jeanne M., Yoo, Andrew S.. Generation of Human Striatal Neurons by MicroRNA-Dependent Direct Conversion of Fibroblasts. Neuron, vol.84, no.2, 311-323.

상세보기
Yoo, Andrew S., Sun, Alfred X., Li, Li, Shcheglovitov, Aleksandr, Portmann, Thomas, Li, Yulong, Lee-Messer, Chris, Dolmetsch, Ricardo E., Tsien, Richard W., Crabtree, Gerald R.. MicroRNA-mediated conversion of human fibroblasts to neurons. Nature, vol.476, no.7359, 228-231.

상세보기
Lee, Yong Seung, Heo, Hyejung, Lee, Jonghwan, Moon, Sung Ung, Jung, Woon Yong, Park, Yong Keun, Park, Min Geun, Oh, Seung-Hun, Kim, Soonhag. An ultra-effective method of generating extramultipotent cells from human fibroblasts by ultrasound. Biomaterials, vol.143, 65-78.

상세보기
Al-Dosari, Mohammed S., Gao, Xiang. Nonviral Gene Delivery: Principle, Limitations, and Recent Progress. The AAPS journal, vol.11, no.4, 671-.

상세보기
Seow, Yiqi, Wood, Matthew J. Biological Gene Delivery Vehicles: Beyond Viral Vectors. Molecular therapy : the journal of the American Society of Gene Therapy, vol.17, no.5, 767-777.

상세보기
Lobb, Richard J., Becker, Melanie, Wen Wen, Shu, Wong, Christina S. F., Wiegmans, Adrian P., Leimgruber, Antoine, Möller, Andreas. Optimized exosome isolation protocol for cell culture supernatant and human plasma. Journal of extracellular vesicles, vol.4, no.1, 10.3402/jev.v4.27031-.

상세보기

LOADING...

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증