[논문]Relationships between and formation dynamics of the microbiota of consumers, producers, and the environment in an abalone aquatic system

Jiang, Jing-Zhe; Zhao, Wang; Liu, Guang-Feng; Wang, Jiang-Yong

doi:10.1371/journal.pone.0182590

[해외논문] Relationships between and formation dynamics of the microbiota of consumers, producers, and the environment in an abalone aquatic system 원문보기

PloS one, v.12 no.8, 2017년, pp.e0182590 -

Jiang, Jing-Zhe (Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China) , Zhao, Wang (Tropical Fisheries Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, China) , Liu, Guang-Feng (Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China) , Wang, Jiang-Yong (Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China)

Abstract ▼ AI-Helper

An ecosystem is a community comprising living and nonliving components of the environment. Microbes are ubiquitous elements in each of these components. The dynamics of microbiota formation in an ecosystem is important to elucidate, because how the different components of a system exchange microbes, and how the microbes control ecological processes remain unresolved. In this study, an abalone, Haliotis diversicolor, seed-nursing pond was used as a model system. We first examined changes in bacterial communities during the seedling cultivation of this herbivorous juvenile aquatic invertebrate animal. Denaturing gradient gel electrophoresis (DGGE) and pyrosequencing were used to analyze bacterial community dynamics and spatio-temporal interactions of different system components: consumers (abalone), producers (algae or a substrate), and the environment (water). DGGE fingerprints revealed that the developmental stages of abalone influences bacterial communities of both the abalone and substrate. Although the communities in water fluctuated daily, they could be divided into two clusters that coincided with abalone stages, reflecting the transition from larva to juvenile at around day 21. Pyrosequencing showed that the microbiota in the abalone and substrate had more operational taxonomic units in common than that of either with water. The Bray-Curtis similarity index was used to quantify the formation dynamics of microbiota among the various components of the system. The bacterial communities in producers and consumers showed similar changes. These communities were unstable at the beginning and then slowly stabilized over time. The environmental bacterial community was more stable than the bacterial communities in consumers and producers, and may have been the basis for stability in the system. Our research provides insights into the dynamics of microbiota formation in various biotic elements of a system that will contribute to predictive systems modeling.

참고문헌 (37)

1 Chapin FS , Matson PA. PA , Vitousek PM. PM , Stuart Chapin F. Iii , Matson PA. PA , Vitousek PM. PM . Principles of terrestrial ecosystem ecology . Principles of Terrestrial Ecosystem Ecology . 2012 ; doi: 10.1007/978-1-4419-9504-9
2 Zhang GF , Que HY , Liu X , Xu HS . Abalone mariculture in China . J Shellfish Res . 2004 ; 23 : 947 – 950

상세보기
3 Zhao W , Jiang JZ , Wang JY , Chen T , Liu GF , Wang R X , et al Analysis of the bacterial community in the phycosphere of the attachment substances of larval abalone (Haliotis diversicolor Reeve) by PCR-DGGE . J Fish Sci China . 2013 ; 20 : 1225 – 1233
4 Bowler C , Karl DM , Colwell RR . Microbial oceanography in a sea of opportunity . Nature . 2009 ; 459 : 180 – 184 . doi: 10.1038/nature08056 19444203

상세보기
5 Li YF , Chen YR , Yang JL , Bao WY , Guo XP , Liang X , et al Effects of substratum type on bacterial community structure in biofilms in relation to settlement of plantigrades of the mussel Mytilus coruscus . Int Biodeterior Biodegrad . 2014 ; 96 : 41 – 49 . doi: 10.1016/j.ibiod.2014.08.012
6 Bell W , Mitchell R . Chemotactic and growth responses of marine bacteria to algal extracellular products . Biol Bull . 1972 ; 143 : 265 – 277 . doi: 10.2307/1540052

상세보기
7 Amin S a S , Parker MSM , Armbrust EV . Interactions between diatoms and bacteria . Microbiol Mol Biol Rev . 2012 ; 76 : 667 – 684 . doi: 10.1128/MMBR.00007-12 22933565

상세보기
8 Campbell AH , Meritt DW , Franklin RB , Boone EL , Nicely CT , Brown BL . Effects of age and composition of field-produced biofilms on oyster larval setting . Biofouling . 2011 ; 27 : 255 – 65 . doi: 10.1080/08927014.2011.560384 21360359

상세보기
9 The NIH HMP Working Group . The NIH Human Microbiome Project . Genome Res . 2009 ; 19 : 2317 – 2323 . doi: 10.1101/gr.096651.109 19819907

상세보기
10 Matamoros S , Gras-Leguen C , Le Vacon F , Potel G , de La Cochetiere MF . Development of intestinal microbiota in infants and its impact on health . Trends Microbiol . 2013 ; 21 : 167 – 173 . doi: 10.1016/j.tim.2012.12.001 23332725
11 Lokmer A , Goedknegt MA , Thieltges DW , Fiorentino D , Kuenzel S , Baines JF , et al Spatial and temporal dynamics of pacific oyster hemolymph microbiota across multiple scales . Front Microbiol. 2016 ; 7 : 1 – 18 . doi: 10.3389/fmicb.2016.01367 27630625
12 You WW , Ke CH , Luo X , Wang DX . Divergent selection for shell length in two stocks of small abalone Haliotis diversicolor . Aquac Res . 2010 ; 41 : 921 – 929 . doi: 10.1111/j.1365-2109.2009.02376.x

상세보기
13 Buchholz-Cleven BEE , Rattunde B , Straub KL . Screening for Genetic Diversity of Isolates of Anaerobic Fe(II)-oxidizing Bacteria Using DGGE and Whole-cell Hybridization . Syst Appl Microbiol . 1997 ; 20 : 301 – 309 . doi: 10.1016/S0723-2020(97)80077-X

상세보기
14 Muyzer G , Waal EC De , Uitierlinden AG . Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA . Appl Environ Microbiol . 1993 ; 59 : 695 – 700 . doi: 0099-2240/93/030695-06$02.00/0 7683183

상세보기
15 Liu J , Sui Y , Yu Z , Shi Y , Chu H , Jin J , et al High throughput sequencing analysis of biogeographical distribution of bacterial communities in the black soils of northeast China . Soil Biol Biochem . 2014 ; 70 : 113 – 122 . doi: 10.1016/j.soilbio.2013.12.014

상세보기
16 Krebs C . Ecology: The Experimental Analysis of Distribution and Abundance . New York : Harper and Row 1978
17 Lozupone CA , Knight R . UniFrac: a New Phylogenetic Method for Comparing Microbial Communities UniFrac: a New Phylogenetic Method for Comparing Microbial Communities . Appl Environ Microbiol . 2005 ; 71 : 8228 – 8235 . doi: 10.1128/AEM.71.12.8228-8235.2005 16332807
18 Schloss PD , Westcott SL , Ryabin T , Hall JR , Hartmann M , Hollister EB , et al Introducing mothur: Open—Source, Platform—Independent, Community—Supported Software for Describing and Comparing Microbial Communities . Appl Environ Microbiol . 2009 ; 75 : 7537 – 7541 . doi: 10.1128/AEM.01541-09 19801464

상세보기
19 Bray RJ , Curtis JT . An ordination of the upland forest communities of southern Winsconin . Ecol Monogr . 1957 ; 27 : 325 – 349 . doi: 10.2307/1942268
20 Bloom SA . Similarity indices in community studies: potential pitfalls . Mar Ecol Prog Ser . 1981 ; 5 : 125 – 128 . doi: 10.3354/meps005125
21 Thomas JC , Wafula D , Chauhan A , Green SJ , Gragg R , Jagoe C . A survey of deepwater horizon (DWH) oil-degrading bacteria from the Eastern oyster biome and its surrounding environment . Front Microbiol . 2014 ; 5 doi: 10.3389/fmicb.2014.00149 24782841

상세보기
22 Ingerslev HC , von Gersdorff Jørgensen L , Lenz Strube M , Larsen N , Dalsgaard I , Boye M , et al The development of the gut microbiota in rainbow trout (Oncorhynchus mykiss) is affected by first feeding and diet type . Aquaculture . 2014 ; 12 : 032 doi: 10.1016/j.aquaculture.2013.12.032
23 Palmer C , Bik EM , DiGiulio DB , Relman DA , Brown PO . Development of the human infant intestinal microbiota . PLoS Biol . 2007 ; 5 : 1556 – 73 . doi: 10.1371/journal.pbio.0050177 17594176
24 Yatsunenko T , Rey FE , Manary MJ , Trehan I , Dominguez-Bello MG , Contreras M , et al Human gut microbiome viewed across age and geography . Nature . 2009 ; 486 : 222 – 227 . doi: 10.1038/nature07540.A
25 Morelli L . Postnatal Development of Intestinal Microflora as Influenced by Infant Nutrition . J Nutr . 2008 ; doi: 138/9S-II/1791S 18716188
26 Lee MJ , Lee JJ , Han YC , Sang HC , Kim BS . Analysis of microbiota on abalone (Haliotis discus hannai) in South Korea for improved product management . Int J Food Microbiol . 2016 ; 234 : 45 – 52 . doi: 10.1016/j.ijfoodmicro.2016.06.032 27371902

상세보기
27 Trabal Fernández N , Mazón-Suástegui JM , Vázquez-Juárez R , Ascencio-Valle F , Romero J . Changes in the composition and diversity of the bacterial microbiota associated with oysters (Crassostrea corteziensis, Crassostrea gigas and Crassostrea sikamea) during commercial production . FEMS Microbiol Ecol . 2014 ; 88 : 69 – 83 . doi: 10.1111/1574-6941.12270 24325323

상세보기
28 McFrederick QS , Thomas JM , Neff JL , Vuong HQ , Russell KA , Hale AR , et al Flowers and Wild Megachilid Bees Share Microbes . Microb Ecol . 2017 ; 1 : 13 doi: 10.1007/s00248-016-0838-1 27592345
29 Kau AL , Ahern PP , Griffin NW , Goodman AL , Gordon JI . Human nutrition, the gut microbiome and the immune system . Nature . 2011 ; 474 : 327 – 336 . doi: 10.1038/nature10213 21677749

상세보기
30 WEST PA HEWITT JH , MURPHY OM . The Influence of Methods of Collection and Storage on the Bacteriology of Human Milk . J Appl Bacteriol . 1979 ; 46 : 269 – 277 . doi: 10.1111/j.1365-2672.1979.tb00820.x 572360
31 Martín R , Jiménez E , Heilig H , Fernández L , Marín ML , Zoetendal EG , et al Isolation of bifidobacteria from breast milk and assessment of the bifidobacterial population by PCR-denaturing gradient gel electrophoresis and quantitative real-time PCR . Appl Environ Microbiol . 2009 ; 75 : 965 – 969 . doi: 10.1128/AEM.02063-08 19088308

상세보기
32 Martin V , Maldonado-Barragan a. , Moles L , Rodriguez-Banos M , Campo RD , Fernandez L , et al Sharing of Bacterial Strains Between Breast Milk and Infant Feces . J Hum Lact . 2012 ; 28 : 36 – 44 . doi: 10.1177/0890334411424729 22267318

상세보기
33 Penders J , Thijs C , Vink C , Stelma FF , Snijders B , Kummeling I , et al Factors Influencing the Composition of the Intestinal Microbiota in Early Infancy . Pediatrics . 2006 ; 118 : 511 – 521 . doi: 10.1542/peds.2005-2824 16882802

상세보기
34 Koenig JE , Spor A , Scalfone N , Fricker AD , Stombaugh J , Knight R , et al Succession of microbial consortia in the developing infant gut microbiome . Proc Natl Acad Sci U S A . 2011 ; 108 : 4578 – 4585 . doi: 10.1073/pnas.1000081107 20668239

상세보기
35 Favier CF , Vaughan EE , De Vos WM , Akkermans ADL . Molecular monitoring of succession of bacterial communities in human neonates . Appl Environ Microbiol . 2002 ; 68 : 219 – 226 . doi: 10.1128/AEM.68.1.219-226.2002 11772630

상세보기
36 Lokmer A , Kuenzel S , Baines JF , Wegner KM . The role of tissue-specific microbiota in initial establishment success of Pacific oysters . Environ Microbiol . 2016 ; 18 : 970 – 987 . doi: 10.1111/1462-2920.13163 26695476
37 Dominguez-Bello MG , Costello EK , Contreras M , Magris M , Hidalgo G , Fierer N , et al Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns . Proc Natl Acad Sci U S A . 2010 ; 107 : 11971 – 11975 . doi: 10.1073/pnas.1002601107 20566857

상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

[해외논문] Relationships between and formation dynamics of the microbiota of consumers, producers, and the environment in an abalone aquatic system 원문보기

Abstract ▼ AI-Helper

참고문헌 (37)

이 논문을 인용한 문헌

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트