[논문]Comparison of the Commercial QuantiFERON-CMV and Overlapping Peptide-based ELISPOT Assays for Predicting CMV Infection in Kidney Transplant Recipients

Kwon, Ji-Soo; Kim, Taeeun; Kim, Sun-Mi; Sung, Heungsup; Shin, Sung; Kim, Young Hoon; Shin, Eui-Cheol; Kim, Sung-Han; Han, Duck Jong

doi:10.4110/in.2017.17.5.317

Comparison of the Commercial QuantiFERON-CMV and Overlapping Peptide-based ELISPOT Assays for Predicting CMV Infection in Kidney Transplant Recipients 원문보기

Immune network : official journal of the Korean association of immunobiologists, v.17 no.5, 2017년, pp.317 - 325

Kwon, Ji-Soo (Biomedical Science and Engineering Interdisciplinary Program, KAIST, Daejeon, Korea .) , Kim, Taeeun (Department of Infectious Disease, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea .) , Kim, Sun-Mi (Department of Infectious Disease, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea .) , Sung, Heungsup (Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea .) , Shin, Sung (Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea .) , Kim, Young Hoon (Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea .) , Shin, Eui-Cheol (Biomedical Science and Engineering Interdisciplinary Program, KAIST, Daejeon, Korea .) , Kim, Sung-Han (Department of Infectious Disease, Asan Medical Center, University of Ulsan College of Medicine, Se) , Han, Duck Jong

Abstract ▼ AI-Helper

Cytomegalovirus (CMV) is one of the most important opportunistic infections in transplant recipients. Tests for CMV-specific T cell responses have been proposed to change the current risk stratification strategy using CMV assays. We evaluated the usefulness of pre-transplant CMV-specific T cell assays in kidney transplant (KT) candidates for predicting the development of CMV infection after transplantation comparing the results of the overlapping peptides (OLPs)-based enzyme-linked immunospot (ELISPOT) assay and the commercial QuantiFERON-CMV assay. We prospectively enrolled all cases of KT over a 5-month period, except donor CMV-seropositive and recipient seronegative transplants that are at highest risk of CMV infection. All the patients underwent QuantiFERON-CMV, CMV OLPs-based pp65, and immediate-early 1 (IE-1)-specific ELISPOT assays before transplantation. The primary outcome was the incidence of CMV infection at 6 months after transplant. The total of 47 KT recipients consisted of 45 living-donor KTs and 2 deceased-donor KTs. There was no association between positive QuantiFERON-CMV results and CMV infection. However, 10 of 34 patients with phosphoprotein 65 (pp65)- or IE-1-specific ELISPOT results higher than cut-off value developed CMV infections compared with none of 13 patients with results lower than cut-off value developed CMV. The OLPs-based ELISPOT assays are more useful than the QuantiFERON-CMV assay for predicting CMV infection. Patients with higher CMV-specific T cell immunity at baseline appear to be more likely to develop CMV infections after KT, suggesting that the abrupt decline in CMV-specific T cell responses after immunosuppression, or high CMV-specific T cell responses due to frequent CMV activation before KT, may promote CMV infection.

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참고문헌 (20)

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Comparison of the Commercial QuantiFERON-CMV and Overlapping Peptide-based ELISPOT Assays for Predicting CMV Infection in Kidney Transplant Recipients 원문보기

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Comparison of the Commercial QuantiFERON-CMV and Overlapping Peptide-based ELISPOT Assays for Predicting CMV Infection in Kidney Transplant Recipients 원문보기

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