[논문]Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering

Huh, Man-Il; Lee, Kyoung-Pil; Kim, Jeongho; Yi, Soojin; Park, Byeong-Ung; Kim, Hong Kyun

doi:10.1155/2018/2590536

Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering 원문보기

Journal of ophthalmology, v.2018, 2018년, pp.2590536 -

Huh, Man-Il (Bio-Medical Institute, Kyungpook National University Hospital, Daegu, Republic of Korea) , Lee, Kyoung-Pil (Bio-Medical Institute, Kyungpook National University Hospital, Daegu, Republic of Korea) , Kim, Jeongho (Department of Ophthalmology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea) , Yi, Soojin (Department of Ophthalmology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea) , Park, Byeong-Ung (Department of Ophthalmology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea) , Kim, Hong Kyun (Bio-Medical Institute, Kyungpook National University Hospital, Daegu, Republic of Korea)

Abstract ▼ AI-Helper

Purpose To establish an optimized and standardized protocol for the development of optimal scaffold for bioengineering corneal substitutes, we used femtosecond laser to process human corneal tissue into stromal lenticules and studied to find the most efficient decellularization method among various reagents with different tonicities. Methods The decellularization efficacy of several agents (0.1%, 0.25%, and 0.5% of Triton X-100, SDS, and trypsin-EDTA (TE), resp.) with different tonicities was evaluated. Of all protocols, the decellularization methods, which efficiently removed nuclear materials examined as detected by immunofluorescent staining, were quantitatively tested for sample DNA and glycosaminoglycan (GAG) contents, recellularization efficacy, and biocompatibilities. Results 0.5% SDS in hypertonic and isotonic buffer, 0.25% TE in hypotonic buffer, and 0.5% TE in all tonicities completely decellularized the corneal lenticules. Of the protocols, decellularization with hypotonic 0.25 and 0.5% TE showed the lowest DNA contents, while the GAG content was the highest. Furthermore, the recellularization efficacy of the hypotonic TE method was better than that of the SDS-based method. Hypotonic TE-treated decellularized corneal lenticules (DCLs) were sufficiently transparent and biocompatible. Conclusion We generated an ideal protocol for DCLs using a novel method. Furthermore, it is possible to create a scaffold using a bioengineered corneal substitute.

참고문헌 (34)

1 Ayres B. D. Rapuano C. J. Refractive power of the cornea Comprehensive Ophthalmology Update 2006 7 5 243 251 17132433
2 Kruse F. E. Cursiefen C. Surgery of the cornea: corneal, limbal stem cell and amniotic membrane transplantation Developments in Ophthalmology 2008 41 159 170 10.1159/000131087 2-s2.0-47949109622 18453767

상세보기
3 McColgan K. Corneal transplant surgery Journal of Perioperative Practice 2009 19 2 51 54 10.1177/175045890901900201 19266875

상세보기
4 Melles G. R. J. Remeijer L. Geerards A. J. M. Beekhuis W. H. The future of lamellar keratoplasty Current Opinion in Ophthalmology 1999 10 4 253 259 10.1097/00055735-199908000-00006 2-s2.0-0033513437 10621532

상세보기
5 Nishida K. Tissue engineering of the cornea Cornea 2003 22 Supplement 1 S28 S34 10.1097/00003226-200310001-00005 14703705

상세보기
6 Liu W. Merrett K. Griffith M. Recombinant human collagen for tissue engineered corneal substitutes Biomaterials 2008 29 9 1147 1158 10.1016/j.biomaterials.2007.11.011 2-s2.0-38349052184 18076983

상세보기
7 Minami Y. Sugihara H. Oono S. Reconstruction of cornea in three-dimensional collagen gel matrix culture Investigative Ophthalmology & Visual Science 1993 34 7 2316 2324 7685009

상세보기
8 Vázquez N. Chacón M. Rodríguez-Barrientos C. A. Human bone derived collagen for the development of an artificial corneal endothelial graft. In vivo results in a rabbit model PLoS One 2016 11 12, article e0167578 10.1371/journal.pone.0167578 2-s2.0-85000783364 27907157

상세보기
9 Cheng X. Pinsky P. M. Mechanisms of self-organization for the collagen fibril lattice in the human cornea Journal of the Royal Society Interface 2013 10 87, article 20130512 10.1098/rsif.2013.0512 2-s2.0-84883250291 23904589

상세보기
10 Grier W. K. Iyoha E. M. Harley B. A. C. The influence of pore size and stiffness on tenocyte bioactivity and transcriptomic stability in collagen-GAG scaffolds Journal of the Mechanical Behavior of Biomedical Materials 2017 65 295 305 10.1016/j.jmbbm.2016.08.034 2-s2.0-84985995774 27614271

상세보기
11 Alaminos M. Sa´nchez-Quevedo M. D. C. Mun~oz-A´vila J. I. Construction of a complete rabbit cornea substitute using a fibrin-agarose scaffold Investigative Ophthalmology & Visual Science 2006 47 8 3311 3317 10.1167/iovs.05-1647 2-s2.0-33748109040 16877396

상세보기
12 González-Andrades M. Garzón I. Gascón M. I. Sequential development of intercellular junctions in bioengineered human corneas Journal of Tissue Engineering and Regenerative Medicine 2009 3 6 442 449 10.1002/term.178 2-s2.0-70350723610 19415764

상세보기
13 Vardar E. Larsson H. M. Engelhardt E. M. IGF-1-containing multi-layered collagen-fibrin hybrid scaffolds for bladder tissue engineering Acta Biomaterialia 2016 41 75 85 10.1016/j.actbio.2016.06.010 2-s2.0-84990961138 27286676

상세보기
14 Crapo P. M. Gilbert T. W. Badylak S. F. An overview of tissue and whole organ decellularization processes Biomaterials 2011 32 12 3233 3243 10.1016/j.biomaterials.2011.01.057 2-s2.0-79951775415 21296410

상세보기
15 Zhao B. Katagiri T. Toyoda H. Heparin potentiates the in vivo ectopic bone formation induced by bone morphogenetic protein-2 Journal of Biological Chemistry 2006 281 32 23246 23253 10.1074/jbc.M511039200 2-s2.0-33747378565 16754660

상세보기
16 Seto S. P. Miller T. Temenoff J. S. Effect of selective heparin desulfation on preservation of bone morphogenetic protein-2 bioactivity after thermal stress Bioconjugate Chemistry 2015 26 2 286 293 10.1021/bc500565x 2-s2.0-84923225521 25621929

상세보기
17 Yu Y. Alkhawaji A. Ding Y. Mei J. Decellularized scaffolds in regenerative medicine Oncotarget 2016 7 36 58671 58683 10.18632/oncotarget.10945 2-s2.0-84988418973 27486772

상세보기
18 Wu Z. Zhou Y. Li N. The use of phospholipase A 2 to prepare acellular porcine corneal stroma as a tissue engineering scaffold Biomaterials 2009 30 21 3513 3522 10.1016/j.biomaterials.2009.03.003 2-s2.0-67349285708 19321202

상세보기
19 Fu Y. Fan X. Chen P. Shao C. Lu W. Reconstruction of a tissue-engineered cornea with porcine corneal acellular matrix as the scaffold Cells Tissues Organs 2010 191 3 193 202 10.1159/000235680 2-s2.0-77952309967 19690400

상세보기
20 Hashimoto Y. Funamoto S. Sasaki S. Preparation and characterization of decellularized cornea using high-hydrostatic pressurization for corneal tissue engineering Biomaterials 2010 31 14 3941 3948 10.1016/j.biomaterials.2010.01.122 2-s2.0-77649275125 20163852

상세보기
21 Xu Y. G. Xu Y. S. Huang C. Feng Y. Li Y. Wang W. Development of a rabbit corneal equivalent using an acellular corneal matrix of a porcine substrate Molecular Vision 2008 14 2180 2189 19052652

상세보기
22 de Araujo A. L. Gomes J. A. Corneal stem cells and tissue engineering: current advances and future perspectives World Journal of Stem Cells 2015 7 5 806 814 10.4252/wjsc.v7.i5.806 26131311

상세보기
23 Vavken P. Joshi S. Murray M. M. TRITON-X is most effective among three decellularization agents for ACL tissue engineering Journal of Orthopaedic Research 2009 27 12 1612 1618 10.1002/jor.20932 2-s2.0-72049124062 19504590

상세보기
24 Ivarsen A. Asp S. Hjortdal J. Safety and complications of more than 1500 small-incision lenticule extraction procedures Ophthalmology 2014 121 4 822 828 10.1016/j.ophtha.2013.11.006 2-s2.0-84898597525 24365175

상세보기
25 Barbaro V. Ferrari S. Fasolo A. Ponzin D. Di Iorio E. Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes Molecular Vision 2009 15 2084 2093 19862337

상세보기
26 Hirayama M. Ogawa M. Oshima M. Functional lacrimal gland regeneration by transplantation of a bioengineered organ germ Nature Communications 2013 4 p. 2497 10.1038/ncomms3497 2-s2.0-84885111868 24084941

상세보기
27 Sharpe J. R. Daya S. M. Dimitriadi M. Martin R. James S. E. Survival of cultured allogeneic limbal epithelial cells following corneal repair Tissue Engineering 2007 13 1 123 132 10.1089/ten.2006.0108 2-s2.0-33846646420 17518586

상세보기
28 Yam G. H.-F. Yusoff N. Z. B. M. Goh T.-W. Decellularization of human stromal refractive lenticules for corneal tissue engineering Scientific Reports 2016 6 1, article 26339 10.1038/srep26339 2-s2.0-84969945414 27210519

상세보기
29 Pang K. Du L. Wu X. A rabbit anterior cornea replacement derived from acellular porcine cornea matrix, epithelial cells and keratocytes Biomaterials 2010 31 28 7257 7265 10.1016/j.biomaterials.2010.05.066 2-s2.0-77955267020 20598368

상세보기
30 Sasaki S. Funamoto S. Hashimoto Y. In vivo evaluation of a novel scaffold for artificial corneas prepared by using ultrahigh hydrostatic pressure to decellularize porcine corneas Molecular Vision 2009 15 2022 2028 19844587

상세보기
31 Meyer S. R. Chiu B. Churchill T. A. Zhu L. Lakey J. R. Ross D. B. Comparison of aortic valve allograft decellularization techniques in the rat Journal of Biomedical Materials Research. Part A 2006 79 2 254 262 10.1002/jbm.a.30777 2-s2.0-33750462398 16817222

상세보기
32 Yang B. Zhang Y. Zhou L. Development of a porcine bladder acellular matrix with well-preserved extracellular bioactive factors for tissue engineering Tissue Engineering Part C: Methods 2010 16 5 1201 1211 10.1089/ten.tec.2009.0311 2-s2.0-77957348359 20170425

상세보기
33 Yoeruek E. Bayyoud T. Maurus C. Reconstruction of corneal stroma with decellularized porcine xenografts in a rabbit model Acta Ophthalmologica 2012 90 3 e206 e210 10.1111/j.1755-3768.2011.02300.x 2-s2.0-84860474749 22136520

상세보기
34 Yoeruek E. Bayyoud T. Maurus C. Decellularization of porcine corneas and repopulation with human corneal cells for tissue-engineered xenografts Acta Ophthalmologica 2012 90 2 e125 e131 10.1111/j.1755-3768.2011.02261.x 2-s2.0-84857782512 22136333

상세보기

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering 원문보기

Abstract ▼ AI-Helper

참고문헌 (34)

이 논문을 인용한 문헌

연구과제 타임라인

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering 원문보기

Abstract ▼ AI-Helper

참고문헌 (34)

이 논문을 인용한 문헌

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트