본 연구는 딸기에서 발생하는 흰가루병 방제에 사용되는 살균제의 잔류시험을 수행하고 회귀방정식 및 생물학적 반감기를 산출하여 한국과 일본, Codex의 잔류허용기준에 근거한 농약의 사용 및 안전한 농산물 생산에 기여하고자 수행되었다. 딸기재배 중 흰가루병 방제용 주요 살균제 boscalid, kresoxim-methyl, pyraclostrobin, pyrimethanil을 각 농약의 안전사용기준에 따라 기준량 1회 살포 후 1, 3, 5, 7, 9, 11, 13일에 딸기 시료를 채취하여 QuEChERS법을 이용하여 추출하고, NH2 SPE 카트리지로 정제하여 HPLC/DAD로 분석하였다. 시험농약의 검출한계 및 정량한계는 4성분의 농약에서 모두 0.01과 0.03mg/kg이었으며, 평균회수율은 92.9~99.2%, 상대표준편차는≤5%로 산출되었다. 딸기 중 boscalid, kresoxim-methyl, pyraclostrobin, pyrimethanil의 생물학적 반감기는 기준량처리 시각각 6.2, 4.0, 6.1, 6.6일로 나타났다. 이러한 결과로, 시설재배지 딸기 흰가루병 방제를 위해 사용되는 살균제의 안전 사용을 통해 국내 및 주요 수출국가의 MRL을 초과하지 않는 안전한 딸기를 생산하는데 기여할 것으로 기대된다.
본 연구는 딸기에서 발생하는 흰가루병 방제에 사용되는 살균제의 잔류시험을 수행하고 회귀방정식 및 생물학적 반감기를 산출하여 한국과 일본, Codex의 잔류허용기준에 근거한 농약의 사용 및 안전한 농산물 생산에 기여하고자 수행되었다. 딸기재배 중 흰가루병 방제용 주요 살균제 boscalid, kresoxim-methyl, pyraclostrobin, pyrimethanil을 각 농약의 안전사용기준에 따라 기준량 1회 살포 후 1, 3, 5, 7, 9, 11, 13일에 딸기 시료를 채취하여 QuEChERS법을 이용하여 추출하고, NH2 SPE 카트리지로 정제하여 HPLC/DAD로 분석하였다. 시험농약의 검출한계 및 정량한계는 4성분의 농약에서 모두 0.01과 0.03mg/kg이었으며, 평균회수율은 92.9~99.2%, 상대표준편차는≤5%로 산출되었다. 딸기 중 boscalid, kresoxim-methyl, pyraclostrobin, pyrimethanil의 생물학적 반감기는 기준량처리 시각각 6.2, 4.0, 6.1, 6.6일로 나타났다. 이러한 결과로, 시설재배지 딸기 흰가루병 방제를 위해 사용되는 살균제의 안전 사용을 통해 국내 및 주요 수출국가의 MRL을 초과하지 않는 안전한 딸기를 생산하는데 기여할 것으로 기대된다.
This study was conducted to investigate the dissipation and residual characteristics of fungicides used for powdery mildew control for strawberry and establishment of guideline for safe use of fungicides based on MRLs as Korea, Japan and Codex. The fungicides were sprayed onto the crop in triplicat...
This study was conducted to investigate the dissipation and residual characteristics of fungicides used for powdery mildew control for strawberry and establishment of guideline for safe use of fungicides based on MRLs as Korea, Japan and Codex. The fungicides were sprayed onto the crop in triplicate at recommended dose. Strawberry samples were harvested at 1, 3, 5, 7, 9, 11 and 13 days after treatment boscalid, kresoxim-methyl, pyraclostrobin, and pyrimethanil were extracted with QuEChERS method, clean-up with NH2 SPE cartridge and residue were analyzed by HPLC/DAD. Limit of detections and quantifications were 0.01 and 0.03 mg/kg for 4 fungicides. Average recoveries were 92.9~99.2% with RSD≤5% for all analytes. The biological half-lives of boscalid, kresoxim-methyl, pyraclostrobin and pyrimethanil in strawberry after treatment were 6.2, 4.0, 6.1 and 6.6 days at recommended dose, respectively. As the results, 4 experimental pesticides were not exceeded MRLs of domestic and exporting countries in experimental periods. This study provide important information that can contribute safety measures to produce strawberry as not exceeded MRLs through the safe use of pesticides.
This study was conducted to investigate the dissipation and residual characteristics of fungicides used for powdery mildew control for strawberry and establishment of guideline for safe use of fungicides based on MRLs as Korea, Japan and Codex. The fungicides were sprayed onto the crop in triplicate at recommended dose. Strawberry samples were harvested at 1, 3, 5, 7, 9, 11 and 13 days after treatment boscalid, kresoxim-methyl, pyraclostrobin, and pyrimethanil were extracted with QuEChERS method, clean-up with NH2 SPE cartridge and residue were analyzed by HPLC/DAD. Limit of detections and quantifications were 0.01 and 0.03 mg/kg for 4 fungicides. Average recoveries were 92.9~99.2% with RSD≤5% for all analytes. The biological half-lives of boscalid, kresoxim-methyl, pyraclostrobin and pyrimethanil in strawberry after treatment were 6.2, 4.0, 6.1 and 6.6 days at recommended dose, respectively. As the results, 4 experimental pesticides were not exceeded MRLs of domestic and exporting countries in experimental periods. This study provide important information that can contribute safety measures to produce strawberry as not exceeded MRLs through the safe use of pesticides.
AgriosGN . 2004. Plant pathology, pp.448-452. 5th ed. Elsevier, USA.
10.1093/jaoac/86.2.412 AnastassiadesMS , LehotayJ , StaJnbaherD and SchenckFJ . 2003. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and ?odispersive solid-phase extraction ?? for the determination of pesticide residues in produce . J. AOAC Int. 86: 412-431.
ChangHS , BaeHR , SonYB , SongIH , LeeCH , ChoiNG , ChoKK and LeeYG . 2011. Developing a web-based system for computing pre-harvest residue limits(PHRLs). . Agribusiness and Information Management. 3: 11-37.
ChoKS , LeeSJ , LeeDY , KimYJ , ChoiWJ , LeeJB and KangKY . 2011. Pre-harvest residual characteristics of boscalid and pyraclostrobin in paprika at different deasons and plant parts . Kor. J. Pesti. Sci. 15: 269-277.
10.1016/j.trac.2011.02.008 FenikJ , TankiewiczM and BiziukM . Properties and determination of pesticides in fruits and vegetables . Trends Analyt Chem. 2011. 30: 814-826.
KimSW , LeeEM , LinY , ParkHW , LeeHR , RiuMJ , NaYR , NohJE , KeumYS , SongHH and KimJH . 2009. Establishment of pre-harvest residue limit(PHRL) of insecticide bifenthrin during cultivationof grape . Kor. J. Pesti. Sci. 13: 241-248.
JeongYH , KimJU , KimJH , LeeYD , LimCH and HurJH . 2004. Sigma-press. Modern pesticide. pp.5. first eds. Republic of Korea.
KATI2015. www.gtis.com.
Korea Crop Protection Association2012. Guidelines of Pesticide Use. pp.137-, 266, 414, 417. Republic of Korea
10.7585/kjps.2015.19.4.361 LeeDY , JeongDK , ChoiGH , LeeDY , KangKY and KimJH . 2015. Residual characteristics of bistrifluron and fluopicolide in Korean cabbage for establishing pre-harvest residue limit . Kor. J. Pesti. Sci. 19: 361-369.
10.5338/KJEA.2013.32.1.84 LeeDY , KimYJ , KimSG and KangKY . 2012. Residual characteristics of insecticides used for oriental tobacco budworm control of paprika . Kor. J. Environ. Agric. 32: 76-86.
LeeYD . 2011. National Institute of Food and Drug Safety Evaluation. Handbook for the pesticide residue analytical methods of Food Code Index. pp.80-82. third eds. Republic of Korea.
10.1093/jaoac/90.2.1SUP LehotaySJ . 2007. Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate: collaborative study . J. AOAC Int. 90: 485-520.
HwangMR , KimHE , ParkDK , HeuYC , LeeHJ and KangNJ . 2013. Induction of oxidative stress and activation of antioxidant enzymes by infection of powdery mildew in Cucurbita plants . J. Agric. Life Sci. 47: 75-81.
10.5423/RPD.2012.18.2.080 NamMH , JeonYN , LeeHC , LeeHD and KangHK . 2012. Comparative analysis between healthy and powdery mildew-infected Plants of strawberry cultivar seolhyang . Research in Plant Disease. 18: 80-85.
ParkDS , SeongKY , ChoiKI and HurJH . 2005. Field tolerance of pesticides in the strawberry and comparison of biological half-lives estimated from kinetic models . Kor. J. Pesti. Sci. 9: 231-236.
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