[논문]Dichloromethane Extracts of Geranium Koreanum Kom. Alleviates Esophagus Damage in Acute Reflux Esophagitis-Induced Rats by Anti-Inflammatory Activities

Nam, Hyeon Hwa; Nan, Li; Choo, Byung Kil

doi:10.3390/ijms19113622

[해외논문] Dichloromethane Extracts of Geranium Koreanum Kom. Alleviates Esophagus Damage in Acute Reflux Esophagitis-Induced Rats by Anti-Inflammatory Activities 원문보기

International journal of molecular sciences, v.19 no.11, 2018년, pp.3622 -

Nam, Hyeon Hwa , Nan, Li , Choo, Byung Kil

Abstract ▼ AI-Helper

Reflux esophagitis (RE) is a gastrointestinal disease caused by the reflux of gastric acid and stomach contents, and it leads to esophageal damage. Therefore, it is necessary to study the improvement of esophageal damage on a RE-induced model. The present study was accomplished to demonstrate the protective effects of a dichloromethane fraction of Geranium koreanum (DGK) plant on esophageal damage in an acute RE rat model. First, we examined the potential of anti-inflammatory effects of various fractions measured by cell cytotoxicity, morphological changes and nitric oxide (NO) production on lipopolysaccharide (LPS)-induced Raw 264.7 macrophage cells. Then, to evaluate the protective effects on RE, rats were partitioned into the following groups: normal control, RE-induced control and RE rats pre-treated with DGK 100 and 200 mg/kg body weight. The esophageal mucosal ulcer ratio was measured by the Image J program and histological changes were examined using a hematoxylin and eosin staining of the esophageal mucosa. The expression of pro-inflammatory proteins, cytokines and tight junction proteins involved in the esophageal mucosal damage were investigated using Western blotting and an enzyme-linked immunosorbent assay (ELISA) kit with esophagus tissue. DGK chemical profile and phenolic contents were analyzed by liquid chromatography-mass spectrometry (LC-MS/MS). The results showed that DGK exhibited anti-inflammatory effects against LPS-stimulated cells by significantly inhibiting NO production. Additionally, the results in vivo showed that improvement effects of DGK on esophageal mucosal damage. The expression of inflammatory proteins involved in nuclear factor κB (NF-κB) signaling pathways and tight junction protein (claudin-4 and -5) were significantly decreased in esophageal mucosa. We found the potential of DGK as source of replacement therapy products for inflammatory and RE disease.

주제어

참고문헌 (41)

1. Mahattanadul S. Ridtitid W. Nima S. Phdoongsombut N. Ratanasuwon P. Kasiwong S. Effects of morinda citrifolia aqueous fruit extract and its biomarker scopoletin on reflux esophagitis and gastric ulcer in rats J. Ethnopharmacol. 2011 134 243 250 10.1016/j.jep.2010.12.004 21163341

상세보기
2. Yoshida N. Inflammation and oxidative stress in gastroesophageal reflux disease J. Clin. Biochem. Nutr. 2007 40 13 23 10.3164/jcbn.40.13 18437209

상세보기
3. Kim M.Y. Shin Y.O. Lee J.Y. Lee A.R. Shin S.H. Kwon O.J. Seo B.I. Roh S.S. Improving effect of a combined extract of rhei rhizoma and glycyrrhizae rhizoma through anti-oxidative stress in reflux esophagitis rats Kor. J. Herbol. 2015 30 37 44 10.6116/kjh.2015.30.4.37
4. Meining A. Classen M. The role of diet and lifestyle measures in the pathogenesis and treatment of gastroesophageal reflux disease Am. J. Gastroenterol. 2000 95 2692 2697 10.1111/j.1572-0241.2000.03175.x 11051337

상세보기
5. Yoshida N. Kamada K. Tomasturi N. Suzuki T. Takagi T. Ichikawa H. Yoshikawa T. Management of recurrence of symptoms of gastroesophageal reflux disease: Synergistic effect of rebamipide with 15 mg lansoprazole Dig. Dis. Sci. 2010 55 3393 3398 10.1007/s10620-010-1166-9 20198424

상세보기
6. Layli E. Nasseri-Moghaddam S. Meta-analyses: Does long-term PPI use increase the risk of gastric premalignant lesions? Arch. Iran. Med. 2013 16 449 458 23906249

상세보기
7. Jabri M.A. Tounsi H. Abdellaoui A. Marzouki L. Sebai H. Protective effects of Artemisia campestris extract against gastric acid reflux-induced esophageal mucosa injuries Pathophysiology 2018 25 63 69 10.1016/j.pathophys.2018.01.001 29306510

상세보기
8. Lian B. Liu Z.H. Wei H.T. Zhang Z. Shi G.N. Protection effect Xuanfudaizhetang on reflux esophagitis in rats Asian Pac. J. Trop. Med. 2014 7 267 270 10.1016/S1995-7645(14)60035-3 24507673

상세보기
9. Shin M.R. Seo B.I. Son C.G. Roh S.S. An H.J. Banhasasim-tang treatment reduces the severity of esophageal mucosal ulcer on chronic acid reflux esophagitis in rats BioMed Res. Int. 2017 7157212 10.1155/2017/7157212 28349065

상세보기
10. Wang P. Qiao Q. Wang J.L. Yao L.P. Fu Y.J. Inhibitory effects of geraniin on LPS-induced inflammation via regulating NF-κB and Nrf2 pathways in RAW 264.7 Cells Chem. Biol. Interact. 2016 253 134 142 10.1016/j.cbi.2016.05.014 27181634

상세보기
11. Hossen M.J. Cho J.Y. Kim D.W. PDK1 in NF-κB signaling is a target of Xanthium strumarium methanolic extract-mediated anti-inflammatory activities J. Ethnopharmacol. 2016 190 251 260 10.1016/j.jep.2016.06.019 27286918

상세보기
12. Huang B.P. Lin C.H. Chen Y.C. Kao S.H. Anti-inflammatory effects of Perilla frutescens leaf extract on lipopolysaccaharide-stimulated RAW 264.7 cells Mol. Med. Rep. 2014 10 1077 1083 10.3892/mmr.2014.2298 24898576

상세보기
13. Souza R.F. Huo X. Mittal V. Schuler C.M. Carmack S.W. Zhang H.Y. Zhang X. Yu C. Hormi-Carver K. Genta R.M. Gastroesophageal reflux might cause esophagitis through a cytokine-mediated mechanism rather than caustic acid injury Gastroenterology 2009 137 1776 1784 10.1053/j.gastro.2009.07.055 19660463

상세보기
14. Kang J.W. Lee S.M. Protective effects of chlorogenic acid against experimental reflux esophagitis in rats Biomol. Ther. 2014 22 420 425 10.4062/biomolther.2014.066 25414772

원문보기 상세보기
15. Klein A. Kluge S. Peskar B.M. Stimulation of prostaglandin biosynthesis mediates gastroprotective effect of rebamipide in rats Dig. Dis. Sci. 1933 38 1441 1449 10.1007/BF01308601

상세보기
16. Katada D. Yoschida N. Isozaki Y. Tomatsuri N. Ichikawa H. Naito Y. Okanoue T. Yoshikawa T. Prevention by rebamipide of acute reflux esophagitis in rats Dig. Dis. Sci. 2005 50 97 103 10.1007/s10620-005-2813-4 16184428

상세보기
17. Fitzgerald R.C. Onwuegbusi B.A. Majaj-Elliott M. Saeed I.T. Burnham W.R. Farthing M.J.G. Diversity in the oesophageal phenotypic response to gastro-oesophageal reflux: Immunological determinants Gut 2002 50 451 459 10.1136/gut.50.4.451 11889061

상세보기
18. Miwa H. Koseki J. Oshima T. Kondo T. Tomita T. Watari J. Matsumoto T. Hattori T. Kubota K. Iizuka S. Rikkunshito, a traditional Japanese medicine, may relieve abdominal symptoms in rats with experimental esophagitis by improving the barrier function of epithelial cells in esophageal mucosa J. Gastroenterol. 2010 45 478 487 10.1007/s00535-009-0180-1 20016992

상세보기
19. Burek M. Aras-Loza P.A. Roewer N. Forster C.Y. Claudin-5 as a novel estrogen target in vascular endothelium Arterioscler. Thromb. Vasc. Biol. 2010 30 298 304 10.1161/ATVBAHA.109.197582 19910637

상세보기
20. Poliak S. Matlis S. Ullmer C. Scherer S.S. Peles E. Distinct claudins and associated PDZ proteins form different autotypic tight junctions in myelinating Schwann cells J. Cell Ciol. 2002 159 361 372 10.1083/jcb.200207050 12403818

상세보기
21. Amasheh S. Dullat S. Fromm M. Schulzke J.D. Buhr H.J. Kroesen A.J. Inflamed pouch mucosa possesses altered tight junctions indicating recurrence of inflammatory bowel disease Int. J. Colorectal. Dis. 2009 24 1149 1156 10.1007/s00384-009-0737-8 19488769

상세보기
22. Jung H. Jun K.H. Jung J.H. Chin H.M. Park W.B. The expression of claudin-1, claudin-2, claudin-3, and claudin-4 in gastric cancer tissue J. Surg. Res. 2011 167 e185 e191 10.1016/j.jss.2010.02.010 20462599

상세보기
23. Sanford J.L. Edwards J.D. Mays T.A. Gong B. Merriam A.P. Rafael-Fortney J.A. Claudin-5 localizes to the lateral membranes of cardiomyocytes and is altered in utrophin/dystrophin-deficient cardiomyophathic mice J. Mol. Cell Cardiol. 2005 38 323 332 10.1016/j.yjmcc.2004.11.025 15698839

상세보기
24. Kupeli E. Tatli I.I. Akdemir Z.S. Yesilada E. Estimation of antinociceptive and anti-inflammatory activity on Geranium pretense subp. Finitimum and its phenolic compounds J. Ethopharmacol. 2007 114 234 240 10.1016/j.jep.2007.08.005 17904777

상세보기
25. Oh J.Y. Lee K.J. Wei B. Roh J.H. Kang M. Cha S.Y. Jang H.K. Antibacterial activities of bark extracts from Fraxinus rhynchophylla Hance and Geranium koreanum Kom. against clinical strains of Clostridium perfringens in chickens Korean J. Vet. Res. 2015 55 117 123 10.14405/kjvr.2015.55.2.117

원문보기 상세보기
26. Xiao F. Zhai Z. Jiang C. Liu X. Li H. Qu X. Quyang Z. Fan Q. Tang T. Qin A. Geraniin suppresses RANKL-induced osteoclastogenesis in vitro and ameliorates wear particle-induced osteolysis in mouse model Exp. Cell Res. 2015 330 91 101 10.1016/j.yexcr.2014.07.005 25016282

상세보기
27. Shim J.U. Oh P.S. Lim K.T. Anti-inflammatory activity of ethanol extract from Geranium sibiricum Linne J. Ethnopharmacol. 2009 126 90 95 10.1016/j.jep.2009.08.004 19683044

상세보기
28. Lu C.H. Li Y.Y. Li L.J. Liang L.Y. Shen Y.M. Anti-inflammatory activities fraction from Geranium nepalense and related polyphenols Drug Discov. Ther. 2013 6 194 196 10.5582/ddt.2012.v6.4.194
29. Choo B.K. Roh S.S. Berberine protects against esophageal mucosal damage in reflux esophagitis by suppressing proinflammatory cytokines Exp. Ther. Med. 2013 6 663 670 10.3892/etm.2013.1202 24137243

상세보기
30. Gweon T.G. Park J.H. Kim B.W. Choi Y.K. Kim J.S. Park S.M. Kim C.W. Kim H.G. Chung J.W. Additive effects of rebamipide plus protone pump inhibitors on the expression of tight junction proteins in a rat model of gastro-esophageal reflux disease Gut. Liver 2017 12 46 50 10.5009/gnl17078 29069891

상세보기
31. Zong Y. Sun L. Liu B. Deng Y.S. Zhan D. Chen Y.L. He Y. Liu J. Zhang Z.J. Sun J. Resveratrol inhibits LPS-induced MAPKs activation via activation of the phosphatidylinositol 3-kinase pathway murine Raw 264.7 macrophage cells PLoS ONE 2012 7 e44107 10.1371/journal.pone.0044107 22952890

상세보기
32. Li Y.W. Sifrim D. Xie C. Chen M. Xiao Y.L. Relationship between salivary pepsin concentration and esophageal mucosal integrity in patients with gastroesophageal reflux disease J. Neurogastroenterol. Motil. 2017 23 517 525 10.5056/jnm16178 28992675

상세보기
33. Hatware K.V. Sharma S. Patil K. Shete M. Karri S. Gupta G. Evidence for gastroprotective, anti-infalmmatory and antioxidant potential of methanolic extract of Cordia dichotoma leaves on indomethacin and stress induced gastric lesions in Wistar rats BioMed Pharmacother. 2018 103 317 325 10.1016/j.biopha.2018.04.007 29660650

상세보기
34. Liu D. Ma Y. Wang Y. Su Z. Gu M. Janson J.C. One-step separation and purification of hydrolysable tannis from Geranium wilfordii Maxim by adsorption Chromatography on cross-linked 12% agarose gel J. Sep. Sci. 2011 34 995 998 10.1002/jssc.201100006 21442751

상세보기
35. Zhu G. Xin X. Liu Y. Huang Y. Li K. Wu C. Geraniin attenuates LPS-induced acute lung injury via inhibiting NF-κB and activating Nrf2 Signaling pathway Oncotarget 2017 8 22835 22841 28423560
36. Wang X. Chen Z. Li X. Jiang Z.K. Zhao Y.Q. Ping F.F. Geraniin suppresses ovarian cancer growth through inhibition of NF-κB activation and downregulation of Mcl-1 expression J. Biochem. Mol. Toxicol. 2017 31 10.1002/jbt.21929 28590547

상세보기
37. Aayadi H. Mittal S.P.K. Deshpande A. Gore M. Ghaskadbi S.S. Cytoprotective effect exerted by geraniin in HepG2 cells is through microRNA mediated regulation of BACH-1 and HO-1 BMB Rep. 2017 50 560 565 10.5483/BMBRep.2017.50.11.060 28602161

원문보기 상세보기
38. Graca V.C. Barros L. Calhelha R.C. Dias M.I. Carvalho A.M. Santos-Buelga C. Santos P.F. Ferreira I.C. Cheminal characterization and bioactive properties of aqueous and organic extracts of Geranium robertianum L. Food Funct. 2016 7 3807 3814 10.1039/C6FO01075J 27603422

상세보기
39. Choi H.J. Choi H.J. Park M.J. Lee J.Y. Jeong S.I. Lee S.G. Kim K.H. Joo M.S. Jeong H.S. Kim J.E. The inhibitory effects of Geranium thunbergii on imterferom-γ- and LPS-induced inflammatory responses are mediated by Nrf2 activation Int. J. Mol. Med. 2015 35 1237 1245 10.3892/ijmm.2015.2128 25761198

상세보기
40. Wu N. Zu Y. Fu Y. Kong Y. Zhao J. Li X. Li J. Wink M. Efferth T. Antioxidant activities and xanthine oxidase inhibitory effects of extracts and main polyphenolic compounds obtained from Geranium Sibiricum L. J. Agric. Food Chem. 2010 58 4737 4743 10.1021/jf904593n 20205393

상세보기
41. Ctarino M.D. Silva A.M.S. Cruz M.T. Cardoso S.M. Antioxidant and anti-inflammatory activities of Geranium robertianum L. decoctions Food Funct. 2017 8 3355 3365 10.1039/C7FO00881C 28858365

상세보기

LOADING...

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

[해외논문] Dichloromethane Extracts of Geranium Koreanum Kom. Alleviates Esophagus Damage in Acute Reflux Esophagitis-Induced Rats by Anti-Inflammatory Activities 원문보기

Abstract ▼ AI-Helper

주제어

참고문헌 (41)

이 논문을 인용한 문헌

연구과제 타임라인

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

[해외논문] Dichloromethane Extracts of Geranium Koreanum Kom. Alleviates Esophagus Damage in Acute Reflux Esophagitis-Induced Rats by Anti-Inflammatory Activities 원문보기

Abstract ▼ AI-Helper

주제어

참고문헌 (41)

이 논문을 인용한 문헌

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트