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Differential detection of porcine reproductive and respiratory syndrome virus genotypes by a fluorescence melting curve analysis using peptide nucleic acid probe-mediated one-step real-time RT-PCR

Journal of virological methods, v.267, 2019년, pp.29 - 34  

Park, Ji-Young (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ,  Kim, Seong-Hee (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ,  Lee, Kyoung-Ki (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ,  Kim, Yeon-Hee (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ,  Moon, Bo-Yeon (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ,  So, ByungJae (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ,  Park, Choi-Kyu (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University)

Abstract ▼ AI-Helper 아이콘AI-Helper

Abstract Peptide nucleic acids (PNAs), artificially synthesized DNA analogues, hybridize strongly with DNA and are useful for fluorescence melting curve analyses (FMCA) based on the thermal denaturation of the probe-target duplex. In this study, we developed a PNA-based one-step real-time RT-PCR as...

주제어

#Porcine reproductive and respiratory syndrome virus   #Peptide nucleic acid   #Fluorescence melting curve analysis   #One-step real-time RT-PCR   #Korea  

참고문헌 (28)

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