[논문]Calpain-dependent Beclin1 cleavage stimulates senescence-associated cell death in HT22 hippocampal cells under the oxidative stress conditions

Nguyen, Huynh Quoc; Zada, Sahib; Lai, Trang Huyen; Pham, Trang Minh; Hwang, Jin Seok; Ahmed, Mahmoud; Kim, Deok Ryong

doi:10.1016/j.neulet.2019.02.036

Calpain-dependent Beclin1 cleavage stimulates senescence-associated cell death in HT22 hippocampal cells under the oxidative stress conditions

Neuroscience letters, v.701, 2019년, pp.106 - 111

Nguyen, Huynh Quoc (Corresponding author.) , Zada, Sahib , Lai, Trang Huyen , Pham, Trang Minh , Hwang, Jin Seok , Ahmed, Mahmoud , Kim, Deok Ryong

Abstract ▼ AI-Helper

Abstract Oxidative damage in neurons including glutamate excitotoxicity has been linked to increasing numbers of neuropathological conditions. Under these conditions, cells trigger several different cellular responses such as autophagy, apoptosis, necrosis and senescence. However, the connection between these responses is not well understood. In this study, we found that the 60-kDa BECN1 was specifically degraded to a 40-kDa fragment in hippocampal HT22 cells treated with 5 mM glutamate. Increased BECN1 cleavage was specifically associated with a decrease in cell viability under oxidative stress. Interestingly, this BECN1 cleavage was specifically inhibited by a calpain inhibitor ALLN but was not affected by other protease inhibitors. Also, the BECN1 cleavage was not detected in calpain-4-deficient cell lines. Furthermore, calpain cleaved BECN1 at a specific site between the coiled-coil domain and Bcl2 homology 3 domain, which is associated with the anti-apoptotic protein Bcl-2. Moreover, some cellular senescence markers, including β-galactosidase, p21, p27Kip1, p53 and p16INK4A, increased proportionally to those of BECN1 cleaved fragments. These results suggest that calpain-mediated BECN1 cleavage under oxidative conditions is specifically associated with cell death induced by cellular senescence. Highlights BECN1 was specifically degraded to a 40-kDa fragment accompanying with decrease of cell viability under oxidative stress. BECN1 cleavage was inhibited by calpain inhibitor but not by other protease inhibitors. Calpain cleaves BECN1 at a specific site between CCD and BH3 domains. BECN1 degradation stimulates senescence-associated cell death at the oxidative stress.

주제어

참고문헌 (21)

Brain Res. Davis 652 169 1994 10.1016/0006-8993(94)90334-4 Protein kinase C activation inhibits glutamate-induced cytotoxicity in a neuronal cell line

상세보기
J. Cell Biol. Tan 141 1423 1998 10.1083/jcb.141.6.1423 The regulation of reactive oxygen species production during programmed cell death

상세보기
Neurosci. Lett. Ha 393 165 2006 10.1016/j.neulet.2005.09.056 Glutamate-induced oxidative stress, but not cell death, is largely dependent upon extracellular calcium in mouse neuronal HT22 cells

상세보기
J. Neurochem. van Leyen 92 824 2005 10.1111/j.1471-4159.2004.02915.x Proteasome inhibition protects HT22 neuronal cells from oxidative glutamate toxicity

상세보기
BMC Neurosci. Zhang 7 49 2006 10.1186/1471-2202-7-49 Glutamate-induced apoptosis in neuronal cells is mediated via caspase-dependent and independent mechanisms involving calpain and caspase-3 proteases as well as apoptosis inducing factor (AIF) and this process is inhibited by equine estrogens

상세보기
Biochem. Biophys. Res. Commun. Kim 388 339 2009 10.1016/j.bbrc.2009.08.007 Activation of autophagy during glutamate-induced HT22 cell death

상세보기
Autophagy Wirawan 8 6 2012 10.4161/auto.8.1.16645 Beclin1: a role in membrane dynamics and beyond

상세보기
J. Virol. Liang 72 8586 1998 10.1128/JVI.72.11.8586-8596.1998 Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein

상세보기
EMBO J. Maiuri 26 2527 2007 10.1038/sj.emboj.7601689 Functional and physical interaction between Bcl-X(L) and a BH3-like domain in Beclin-1

상세보기
Cancer Res. Li 71 3625 2011 10.1158/0008-5472.CAN-10-4475 Following cytochrome c release, autophagy is inhibited during chemotherapy-induced apoptosis by caspase 8-mediated cleavage of Beclin 1

상세보기
PLoS One Kang 6 2011 10.1371/journal.pone.0023367 Autophagy impairment induces premature senescence in primary human fibroblasts

상세보기
Proc. Natl. Acad. Sci. U. S. A. Lipinski 107 14164 2010 10.1073/pnas.1009485107 Genome-wide analysis reveals mechanisms modulating autophagy in normal brain aging and in Alzheimer’s disease

상세보기
Free Radic. Biol. Med. Dumay 40 1377 2006 10.1016/j.freeradbiomed.2005.12.005 The superoxide dismutase inhibitor diethyldithiocarbamate has antagonistic effects on apoptosis by triggering both cytochrome c release and caspase inhibition

상세보기
Methods Mol. Biol. Tompa 144 129 2000 Kinetic analysis of human mu-calpain autolysis

상세보기
Cell Pattingre 122 927 2005 10.1016/j.cell.2005.07.002 Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy

상세보기
J. Biol. Chem. Shibata 281 14474 2006 10.1074/jbc.M600364200 Regulation of intracellular accumulation of mutant Huntingtin by Beclin 1

상세보기
Exp. Gerontol. Unterluggauer 38 1149 2003 10.1016/j.exger.2003.08.007 Senescence-associated cell death of human endothelial cells: the role of oxidative stress

상세보기
Protein Cell Zhu 1 468 2010 10.1007/s13238-010-0048-4 Beclin 1 cleavage by caspase-3 inactivates autophagy and promotes apoptosis

상세보기
Cell Death Dis. Russo 2 e144 2011 10.1038/cddis.2011.29 Calpain-mediated cleavage of Beclin-1 and autophagy deregulation following retinal ischemic injury in vivo

상세보기
Am. J. Physiol. Cell Physiol. Wang 310 C329 2016 10.1152/ajpcell.00231.2015 Calpain activation by ROS mediates human ether-a-go-go-related gene protein degradation by intermittent hypoxia

상세보기
J. Neurosci. Res. Brustovetsky 88 1317 2010 Calpain activation and Na/Ca exchanger degradation occur downstream of calcium deregulation in hippocampal neurons exposed to excitotoxic glutamate

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Calpain-dependent Beclin1 cleavage stimulates senescence-associated cell death in HT22 hippocampal cells under the oxidative stress conditions

Abstract ▼ AI-Helper

주제어

참고문헌 (21)

이 논문을 인용한 문헌

연구과제 타임라인

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

Calpain-dependent Beclin1 cleavage stimulates senescence-associated cell death in HT22 hippocampal cells under the oxidative stress conditions

Abstract ▼ AI-Helper

주제어

참고문헌 (21)

이 논문을 인용한 문헌

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트