[논문]Development of Dual Drug Eluting Cardiovascular Stent with Ultrathin Flexible Poly(L-lactide-co-caprolactone) Coating

Roopmani, Purandhi; Satheesh, Santhosh; Raj, David C.; Krishnan, Uma Maheswari

doi:10.1021/acsbiomaterials.9b00303

[해외논문] Development of Dual Drug Eluting Cardiovascular Stent with Ultrathin Flexible Poly(L-lactide-co-caprolactone) Coating

ACS biomaterials science & engineering, v.5 no.6, 2019년, pp.2899 - 2915

Roopmani, Purandhi (Department of Cardiology , Jawaharlal Institute of Post Graduate Medical Education and Research (JIPMER) , Pondicherry −) , Satheesh, Santhosh (605006 , India) , Raj, David C. , Krishnan, Uma Maheswari

Abstract ▼ AI-Helper

The pleiotropic effects of the atorvastatin-fenofibrate combination can be effectively harnessed for site-specific therapy to minimize stent-related complications. The present study aims to utilize the pleiotropic effects of these two drugs entrapped in a uniform and defect-free coating of poly(L-lactide-co-caprolactone) (PLCL) on a stainless steel stent to overcome stent-associated limitations. The stent coating parameters were optimized using ultrasonic spray coating technique to achieve a thin, smooth, and defect-free dual drug-loaded polymer coating on the stent. The dual drug-loaded polymer coated stent was characterized for surface morphology, thickness and coating integrity. In vitro drug release kinetics of the fabricated stent reveals a sustained release of both drugs for more than 60 days. Significant reduction of thrombus formation and adhesion of lipopolysaccharide-stimulated macrophages on the dual drug containing polymer-coated stent indicates that the drug combination possesses antithrombotic and anti-inflammatory effects. The combination did not adversely influence endothelialization but significantly retarded smooth muscle cell proliferation indicating its potential to overcome restenosis. No bacterial biofilm formation was observed on the stent due to the antibacterial activity of atorvastatin. A rat subcutaneous model was used to evaluate the biocompatibility of the coated stent and compared with the commercial stent. MicroCT, scanning electron microscopy, and morphometric analyses revealed that the coated stents exhibited excellent histocompatibility with no inflammatory response as evidenced from the cytokine levels measured 28 days postimplantation. Our data demonstrates for the first time that the combination of atorvastatin and fenofibrate can be successfully employed in cardiovascular stents to overcome the current limitations of conventional drug-eluting stents.[FIG OMISSION]

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Exp Clin Cardiol Buttar H. S. 229 10 2005

상세보기
Bønaa, Kaare H., Mannsverk, Jan, Wiseth, Rune, Aaberge, Lars, Myreng, Yngvar, Nygård, Ottar, Nilsen, Dennis W., Kløw, Nils-Einar, Uchto, Michael, Trovik, Thor, Bendz, Bjørn, Stavnes, Sindre, Bjørnerheim, Reidar, Larsen, Alf-Inge, Slette, Morten, Steigen, Terje, Jakobsen, Ole J., Bleie, Øyvind, Fossum, Eigil, Hanssen, Tove A., Dahl-Eriksen, Øystein, Njølstad, Inger, Rasmussen, Knut, Wilsgaard, Tom, Nordrehaug, Jan E.. Drug-Eluting or Bare-Metal Stents for Coronary Artery Disease. The New England journal of medicine, vol.375, no.13, 1242-1252.

상세보기
Celik, Turgay, Iyisoy, Atila, Kursaklioglu, Hurkan, Celik, Murat. The forgotten player of in-stent restenosis: Endothelial dysfunction. International journal of cardiology, vol.126, no.3, 443-444.

상세보기
Georgiadou, Panagiota, Voudris, Vassilis. Platelet activation and stent thrombosis. Hellenic journal of cardiology : HJC = Hellēnikē kardiologikē epitheōrēsē, vol.58, no.1, 49-50.

상세보기
Warren, J., Baber, U., Mehran, R.. Antiplatelet therapy after drug-eluting stent implantation. Journal of cardiology, vol.65, no.2, 98-104.

상세보기
Am. Fam Physician Dehmer G. J. 1245 80 2009

상세보기
Pendyala, L.K., Yin, X., Li, J., Chen, J.P., Chronos, N., Hou, D.. The First-Generation Drug-Eluting Stents and Coronary Endothelial Dysfunction. JACC. Cardiovascular interventions, vol.2, no.12, 1169-1177.

상세보기
Secemsky, Eric A., Yeh, Robert W., Kereiakes, Dean J., Cutlip, Donald E., Cohen, David J., Steg, P. Gabriel, Cannon, Christopher P., Apruzzese, Patricia K., D’Agostino Sr, Ralph B., Massaro, Joseph M., Mauri, Laura. Mortality Following Cardiovascular and Bleeding Events Occurring Beyond 1 Year After Coronary Stenting : A Secondary Analysis of the Dual Antiplatelet Therapy (DAPT) Study. JAMA cardiology, vol.2, no.5, 478-.

상세보기
Muramatsu, T., Onuma, Y., Zhang, Y.J., Bourantas, C.V., Kharlamov, A., Diletti, R., Farooq, V., Gogas, B.D., Garg, S., Garcia-Garcia, H.M., Ozaki, Y., Serruys, P.W.. Progress in Treatment by Percutaneous Coronary Intervention: The Stent of the Future. Revista española de cardiología, vol.66, no.6, 483-496.

상세보기
Harumi Higuchi dos Santos, Marilia, Sharma, Abhinav, Sun, Jie‐Lena, Pieper, Karen, McMurray, John J.V., Holman, Rury R., Lopes, Renato D.. International Variation in Outcomes Among People with Cardiovascular Disease or Cardiovascular Risk Factors and Impaired Glucose Tolerance: Insights from the NAVIGATOR Trial. Journal of the American Heart Association : cardiovascular and cerebrovascular disease, vol.6, no.1, e003892-.

상세보기
Huang, Y., Ng, H.C.A., Ng, X.W., Subbu, V.. Drug-eluting biostable and erodible stents. Journal of controlled release : official journal of the Controlled Release Society, vol.193, 188-201.

상세보기
Herrmann, R., Schmidmaier, G., Märkl, B., Resch, A., Hähnel, I., Stemberger, A., Alt, E.. Antithrombogenic Coating of Stents Using a Biodegradable Drug Delivery Technology. Thrombosis and haemostasis, vol.82, no.7, 51-57.

상세보기
Berg, Ryan, Aragon, Joseph, Royter, Vladimir, Shanley, John F., Cogert, Greg, Vermani, Renu, Kar, Saibal, Eigler, Neal, Litvack, Frank. Pimecrolimus and dual pimecrolimus-paclitaxel eluting stents decrease neointimal proliferation in a porcine model. Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions, vol.70, no.6, 871-879.

상세보기
Adriaenssens, T., Mehilli, J., Wessely, R., Ndrepepa, G., Seyfarth, M., Wieczorek, A., Blaich, B., Iijima, R., Pache, J., Kastrati, A., Schomig, A.. Does Addition of Estradiol Improve the Efficacy of a Rapamycin-Eluting Stent?. Journal of the American College of Cardiology, vol.49, no.12, 1265-1271.

상세보기
Thipparaboina, R., Khan, W., Domb, A.J.. Eluting combination drugs from stents. International journal of pharmaceutics, vol.454, no.1, 4-10.

상세보기
Ma, X., Oyamada, S., Gao, F., Wu, T., Robich, M.P., Wu, H., Wang, X., Buchholz, B., McCarthy, S., Gu, Z., Bianchi, C.F., Sellke, F.W., Laham, R.. Paclitaxel/sirolimus combination coated drug-eluting stent: In vitro and in vivo drug release studies. Journal of pharmaceutical and biomedical analysis, vol.54, no.4, 807-811.

상세보기
Raber, L., Serruys, P.W.. Late Vascular Response Following Drug-Eluting Stent Implantation. JACC. Cardiovascular interventions, vol.4, no.10, 1075-1078.

상세보기
Han, Seung Hwan, Quon, Michael J., Koh, Kwang Kon. Beneficial Vascular and Metabolic Effects of Peroxisome Proliferator-Activated Receptor-α Activators. Hypertension, vol.46, no.5, 1086-1092.

상세보기
Ortego, Mónica, Bustos, Carmen, Hernández-Presa, Miguel A, Tuñón, José, Dı́az, Cristina, Hernández, Gonzalo, Egido, Jesús. Atorvastatin reduces NF-κB activation and chemokine expression in vascular smooth muscle cells and mononuclear cells. Atherosclerosis, vol.147, no.2, 253-261.

상세보기
Li, Jingjing, Jiang, Jixin, Yin, Hao, Wang, Lifeng, Tian, Ruijuan, Li, Haijie, Wang, Zengyong, Li, Dong, Wang, Yuebing, Gui, Yu, Walsh, Michael P., Zheng, Xi-Long. Atorvastatin Inhibits Myocardin Expression in Vascular Smooth Muscle Cells. Hypertension, vol.60, no.1, 145-153.

상세보기
Yin, Hao, Gui, Yu, Zheng, Xi-Long. 2-methoxyestradiol inhibits atorvastatin-induced rounding of human vascular smooth muscle cells. Journal of cellular physiology, vol.222, no.3, 556-564.

상세보기
Li, Min, Liu, Yinglin, Dutt, Parmesh, Fanburg, Barry L., Toksoz, Deniz. Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin. American journal of physiology. Lung cellular and molecular physiology, vol.293, no.2, L463-L471.

상세보기
Chen, Shuang, Liu, Baoqin, Kong, Dehui, Li, Si, Li, Chao, Wang, Huaqin, Sun, Yingxian. Atorvastatin Calcium Inhibits Phenotypic Modulation of PDGF-BB-Induced VSMCs via Down-Regulation the Akt Signaling Pathway. PloS one, vol.10, no.4, e0122577-.

상세보기
Int. J. Clin Exp Med. Li C. Y. 220824 9 2016
J. Physiol Pharmacol. Zemankova L. 403 66 2015
Xiao, Hongbing, Qin, Xiong, Ping, Ding, Zuo, Keqiang. Inhibition of Rho and Rac Geranylgeranylation by Atorvastatin Is Critical for Preservation of Endothelial Junction Integrity. PloS one, vol.8, no.3, e59233-.

상세보기
Llevadot, Joan, Asahara, Takayuki. Efecto de las estatinas en la inducción de angiogénesis y vasculogénesis. Revista española de cardiología : publicación oficial de la Sociedad Española de Cardiología, vol.55, no.8, 838-844.

상세보기
Antonopoulos, Alexios S, Margaritis, Marios, Lee, Regent, Channon, Keith, Antoniades, Charalambos. Statins as Anti-Inflammatory Agents in Atherogenesis: Molecular Mechanisms and Lessons from the Recent Clinical Trials. Current pharmaceutical design, vol.18, no.11, 1519-1530.

상세보기
Feng, B., Xu, L., Wang, H., Yan, X., Xue, J., Liu, F., Hu, J.F.. Atorvastatin exerts its anti-atherosclerotic effects by targeting the receptor for advanced glycation end products. Biochimica et biophysica acta. Molecular basis of disease, vol.1812, no.9, 1130-1137.

상세보기
Noonan, Jonathan E., Jenkins, Alicia J., Ma, Jian-Xing, Keech, Anthony C., Wang, Jie Jin, Lamoureux, Ecosse L.. An Update on the Molecular Actions of Fenofibrate and Its Clinical Effects on Diabetic Retinopathy and Other Microvascular End Points in Patients With Diabetes. Diabetes, vol.62, no.12, 3968-3975.

상세보기
Pascal, M., Sepulchre, C., Chazan, J.B., Majoie, B.. Evidence for the inhibition of platelet-derived growth factor induced rat smooth muscle cells DNA synthesis by fenofibric acid at the Go/G₁ cell cycle level. Life sciences, vol.33, no.10, 925-933.

상세보기
Nigro, J., Ballinger, M. L., Dilley, R. J., Jennings, G. L. R., Wight, T. N., Little, P. J.. Fenofibrate modifies human vascular smooth muscle proteoglycans and reduces lipoprotein binding. Diabetologia, vol.47, no.12, 2105-2113.

상세보기
Lee, J.J., Jin, Y.R., Yu, J.Y., Munkhtsetseg, T., Park, E.S., Lim, Y., Kim, T.J., Pyo, M.Y., Hong, J.T., Yoo, H.S., Kim, Y., Yun, Y.P.. Antithrombotic and antiplatelet activities of fenofibrate, a lipid-lowering drug. Atherosclerosis, vol.206, no.2, 375-382.

상세보기
Diep, Quy N., Amiri, Farhad, Touyz, Rhian M., Cohn, Jeffrey S., Endemann, Dierk, Neves, Mario Fritsch, Schiffrin, Ernesto L.. PPARα Activator Effects on Ang II-Induced Vascular Oxidative Stress and Inflammation. Hypertension, vol.40, no.6, 866-871.

상세보기
Rodriguez-Granillo, Agustina, Rubilar, Bibiana, Rodriguez-Granillo, Gaston, Rodriguez, Alfredo E. Advantages and disadvantages of biodegradable platforms in drug eluting stents.. World journal of cardiology, vol.3, no.3, 84-92.

상세보기
Fernandez, J., Etxeberria, A., Ugartemendia, J.M., Petisco, S., Sarasua, J.R.. Effects of chain microstructures on mechanical behavior and aging of a poly(L-lactide-co-ε-caprolactone) biomedical thermoplastic-elastomer. Journal of the mechanical behavior of biomedical materials, vol.12, 29-38.

상세보기
EMJ Thakkar A. S. 114 4 2016 10.33590/emj/10314703
Bose, Sanjukta, Keller, Stephan S., Alstrøm, Tommy S., Boisen, Anja, Almdal, Kristoffer. Process Optimization of Ultrasonic Spray Coating of Polymer Films. Langmuir : the ACS journal of surfaces and colloids, vol.29, no.23, 6911-6919.

상세보기
Routledge, Thomas J., Lidzey, David G., Buckley, Alastair R.. Ultrasonic spray coating as an approach for large-area polymer OLEDs: The influence of thin film processing and surface roughness on electrical performance. AIP advances, vol.9, no.1, 015330-.

상세보기
Shanshan, C., Lili, T., Yingxue, T., Bingchun, Z., Ke, Y.. Study of drug-eluting coating on metal coronary stent. Materials science & engineering. C, Materials for biological applications, vol.33, no.3, 1476-1480.

상세보기
Mohan, A., Narayanan, S., Balasubramanian, G., Sethuraman, S., Krishnan, U.M.. Dual drug loaded nanoliposomal chemotherapy: A promising strategy for treatment of head and neck squamous cell carcinoma. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, vol.99, 73-83.

상세보기
Ramana, L.N., Sharma, S., Sethuraman, S., Ranga, U., Krishnan, U.M.. Stealth anti-CD4 conjugated immunoliposomes with dual antiretroviral drugs - Modern Trojan horses to combat HIV. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, vol.89, 300-311.

상세보기
Biggs, Kevin B., Balss, Karin M., Maryanoff, Cynthia A.. Pore Networks and Polymer Rearrangement on a Drug-Eluting Stent as Revealed by Correlated Confocal Raman and Atomic Force Microscopy. Langmuir : the ACS journal of surfaces and colloids, vol.28, no.21, 8238-8243.

상세보기
Jelonek, Katarzyna, Kasperczyk, Janusz, Li, Suming, Dobrzynski, Piotr, Janeczek, Henryk, Jarzabek, Bozena. Novel Poly(L-lactide-co- ε -caprolactone) Matrices Obtained with the Use of Zr[Acac] ₄ as Nontoxic Initiator for Long-Term Release of Immunosuppressive Drugs. BioMed research international, vol.2013, 607351-.

상세보기
10.2174/138161212803832245
Mikhalovska, Lyuba, Santin, Matteo, Denyer, Stephen, Lloyd, Andrew, G.Teer, Dennis, Field, Sue, Mikhalovsky, Sergey. Fibrinogen adsorption and platelet adhesion to metal and carbon coatings. Thrombosis and haemostasis, vol.92, no.5, 1032-1039.

상세보기
Rodriguez, April L., Wojcik, Brandon M., Wrobleski, Shirley K., Myers Jr., Daniel D., Wakefield, Thomas W., Diaz, Jose A.. Statins, inflammation and deep vein thrombosis: a systematic review. Journal of thrombosis and thrombolysis, vol.33, no.4, 371-382.

상세보기
10.24170/14-1-1865
Ali, Ferhana Y., Armstrong, Paul C.J., Dhanji, Al-Rehan A., Tucker, Arthur T., Paul-Clark, Mark J., Mitchell, Jane A., Warner, Timothy D.. Antiplatelet Actions of Statins and Fibrates Are Mediated by PPARs. Arteriosclerosis, thrombosis, and vascular biology, vol.29, no.5, 706-711.

상세보기
Violi, Francesco, Calvieri, Camilla, Ferro, Domenico, Pignatelli, Pasquale. Statins as Antithrombotic Drugs. Circulation, vol.127, no.2, 251-257.

상세보기
Kadikoylu, Gurhan, Yukselen, Vahit, Yavasoglu, Irfan, Bolaman, Zahit. Hemostatic Effects of Atorvastatin versus Simvastatin. The Annals of pharmacotherapy, vol.37, no.4, 478-484.

상세보기
Cabanel, Mariana, Brand, Camila, Oliveira-Nunes, Maria Cecilia, Cabral-Piccin, Mariela Pires, Lopes, Marcela Freitas, Brito, Jose Marques, de Oliveira, Felipe Leite, El-Cheikh, Marcia Cury, Carneiro, Katia. Epigenetic Control of Macrophage Shape Transition towards an Atypical Elongated Phenotype by Histone Deacetylase Activity. PloS one, vol.10, no.7, e0132984-.

상세보기
Liu, Yan-Cun, Zou, Xian-Biao, Chai, Yan-Fen, Yao, Yong-Ming. Macrophage Polarization in Inflammatory Diseases. International journal of biological sciences, vol.10, no.5, 520-529.

상세보기
Roopmani, Purandhi, Krishnan, Uma Maheswari. Harnessing the pleiotropic effects of atorvastatin-fenofibrate combination for cardiovascular stents. Materials science & engineering. C, Materials for biological applications, vol.92, 875-891.

상세보기
Hogue, Jean-Charles, Lamarche, Benoît, Tremblay, André J., Bergeron, Jean, Gagné, Claude, Couture, Patrick. Differential effect of atorvastatin and fenofibrate on plasma oxidized low-density lipoprotein, inflammation markers, and cell adhesion molecules in patients with type 2 diabetes mellitus. Metabolism : clinical and experimental, vol.57, no.3, 380-386.

상세보기
Tsimihodimos, Vasilis, Kostoula, Angeliki, Kakafika, Anna, Bairaktari, Eleni, Tselepis, Alexandros D., Mikhailidis, Dimitri P., Elisaf, Moses. Effect of Fenofibrate on Serum Inflammatory Markers in Patients With High Triglyceride Values. Journal of cardiovascular pharmacology and therapeutics, vol.9, no.1, 27-33.

상세보기
WANG, X.Q., LUO, N.S., CHEN, Z.Q., LIN, Y.Q., GU, M.N., CHEN, Y.X.. Atorvastatin Attenuates TNF-alpha Production via Heme Oxygenase-1 Pathway in LPS-stimulated RAW264.7 Macrophages. Biomedical and environmental sciences : BES = 生物醫學與環境科學 (英文版), vol.27, no.10, 786-793.

상세보기
Paukkeri, E-L, Leppänen, T, Sareila, O, Vuolteenaho, K, Kankaanranta, H, Moilanen, E. PPARα agonists inhibit nitric oxide production by enhancing iNOS degradation in LPS-treated macrophages. British journal of pharmacology : BJP, vol.152, no.7, 1081-1091.

상세보기
Simon, Daniel I.. Inflammation and Vascular Injury : - Basic Discovery to Drug Development -. Circulation journal : official journal of the Japanese Circulation Society, vol.76, no.8, 1811-1818.

상세보기
Björkhem‐Bergman, Linda, Lindh, Jonatan D., Bergman, Peter. What is a relevant statin concentration in cell experiments claiming pleiotropic effects?. British journal of clinical pharmacology, vol.72, no.1, 164-165.

상세보기
Krysiak, Robert, Gdula-Dymek, Anna, Bachowski, Ryszard, Okopień, Bogusław. Pleiotropic Effects of Atorvastatin and Fenofibrate in Metabolic Syndrome and Different Types of Pre-Diabetes. Diabetes care, vol.33, no.10, 2266-2270.

상세보기
Tex Heart Inst J. Elieson M. 884 39 2012
Lai, Chih-Hung, Lin, Yung-Kai, Lee, Wen-Lieng, Chang, Wei-Chun. Coronary Stent Infection Presented as Recurrent Stent Thrombosis. Yonsei medical journal, vol.58, no.2, 458-461.

상세보기
Ko, Humphrey H.T., Lareu, Ricky R., Dix, Brett R., Hughes, Jeffery D.. Statins: antimicrobial resistance breakers or makers?. PeerJ, vol.5, e3952-.

상세보기
Muhlestein, J.B., May, H.T., Jensen, J.R., Horne, B.D., Lanman, R.B., Lavasani, F., Wolfert, R.L., Pearson, R.R., Yannicelli, H.D., Anderson, J.L.. The Reduction of Inflammatory Biomarkers by Statin, Fibrate, and Combination Therapy Among Diabetic Patients With Mixed Dyslipidemia. Journal of the American College of Cardiology, vol.48, no.2, 396-401.

상세보기
Bessler, Hanna, Salman, Hertzel, Bergman, Michael, Straussberg, Rachel, Djaldetti, Meir. In vitro effect of statins on cytokine production and mitogen response of human peripheral blood mononuclear cells. Clinical immunology : the official journal of the Clinical Immunology Society, vol.117, no.1, 73-77.

상세보기
Won, Tae Wan. Fenofibrate, a peroxisome proliferator-activated receptor α-agonist, blocks lipopolysaccharide-induced inflammatory pathways in mouse liver. Korean journal of hepato-biliary-pancreatic surgery, vol.17, no.3, 89-108.

상세보기
J. Physiol. Pharmacol. Mason R. P. 65 66 2015
Feng, Xiaomeng, Gao, Xia, Jia, Yumei, Zhang, Heng, Xu, Yuan, Wang, Guang. PPAR-α Agonist Fenofibrate Decreased RANTES Levels in Type 2 Diabetes Patients with Hypertriglyceridemia. Medical science monitor : international medical journal of experimental and clinical research, vol.22, 743-751.

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