[논문]Identification and characterization of type III polyketide synthase genes from culturable endophytes of ethnomedicinal plants

Manoharan, Gomathi; Sairam, Thiagarajan; Thangamani, Rajesh; Ramakrishnan, Dhivya; K.Tiwari, Manish; Lee, Jung-Kul; Marimuthu, Jeya

doi:10.1016/j.enzmictec.2019.109396

Identification and characterization of type III polyketide synthase genes from culturable endophytes of ethnomedicinal plants

Enzyme and microbial technology, v.131, 2019년, pp.109396 -

Manoharan, Gomathi (PSG Centre for Molecular Medicine and Therapeutics, PSG Institute of Medical Sciences and Research) , Sairam, Thiagarajan (PSG Centre for Molecular Medicine and Therapeutics, PSG Institute of Medical Sciences and Research) , Thangamani, Rajesh (Biotechnology Division, CSIR- National Engineering and Environmental Research Institute, CMC) , Ramakrishnan, Dhivya (PSG Centre for Molecular Medicine and Therapeutics, PSG Institute of Medical Sciences and Research) , K.Tiwari, Manish (Department of Chemistry, University of Copenhagen) , Lee, Jung-Kul (Department of Chemical Engineering, Konkuk University) , Marimuthu, Jeya (PSG Centre for Molecular Medicine and Therapeutics, PSG Institute of Medical Sciences and Research)

Abstract ▼ AI-Helper

Abstract Endophytic fungi provide benefits to host plants by producing a diverse class of secondary metabolites (natural products). Arrays of polyketide natural products are synthesized by specific classes of polyketide synthases (PKS I, II and III) in host organisms. In the present study, we attempt to screen and identify type III PKSs in culturable fungal endophytes isolated from the ethno medicinal plants including Arbus precatorius, Bacopa monnieri,Citrus aurantifolia and Datura metel to detect the genetic potential of endophytic fungi in producing bioactive compounds. A total of seventeen endophytic fungal strains belonging to eight genera were identified using fungal morphology and rDNA-ITS phylogenetic analyses. A CODEHOP-PCR based strategy was followed to design degenerate primers for the screening of type III PKS genes from fungal endophytes. We had successfully amplified partial PKS genes from eight endophytes. The amplified PKS sequences showed 60–99% identity to already characterized/putative PKS genes. From the partial sequence of FiPKS from Fusarium incarnatum BMER1, a full-length gene was amplified, cloned and characterized. FiPKScDNA was cloned and expressed in E. coli Lemo21 (DE3) and the purified protein was shown to produce pyrones and resorcinols using acyl-CoA thioesters as substrates. FiPKS showed the highest catalytic efficiency of 7.6 × 104 s−1 M-1 with stearoyl CoA as a starter unit. This study reports the identification and characterization of type III PKS from endophytes of medicinal plants by CODEHOP PCR. Highlights A CODEHOP PCR based screening method was employed for type III polyketide synthase gene identification in fungal endophytes. By this approach, partial type III PKS genes from eight fungal endophytes were amplified and sequenced. FiPKS gene from Fusarium incarnatum BMER1, an endophyte of Bacopa monnieri was cloned and functionally characterized. FiPKS produced pyrones and resorcinols with the highest catalytic efficiency of 7.6 x 104 s-1 M-1towards stearoyl CoA.

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참고문헌 (29)

Martinez 445 2008 10.1016/S1572-5995(08)80012-9 Studies in Natural Products Chemistry
Phytomedicine. Russo 12 305 2005 10.1016/j.phymed.2003.12.008 Bacopa monniera, a reputed nootropic plant: An overview

상세보기
Phytomedicine. Sairam 9 207 2002 10.1078/0944-7113-00116 Antidepressant activity of standardized extract of Bacopa monniera in experimental models of depression in rats

상세보기
Int. J. Pharm. Biol. Arch. Sakthi 2 1130 2011 Antibacterial evaluation and phytochemical screening of Datura metel leaf extracts against bacterial pathogens
Biotechnol. Res. Int. Pimentel 2011 1 2010 10.4061/2011/576286 The use of endophytes to obtain bioactive compounds and their application in biotransformation process

상세보기
Phytochem. Rev. Kaul 11 487 2012 10.1007/s11101-012-9260-6 Endophytic fungi from medicinal plants: a treasure hunt for bioactive metabolites

상세보기
Nat. Prod. Rep. Austin 20 79 2003 10.1039/b100917f The chalcone synthase superfamily of type III polyketide synthases

상세보기
Nature. Funa 400 897 1999 10.1038/23748 A new pathway for polyketide synthesis in microorganisms

상세보기
J. Biol. Chem. Funa 282 14476 2007 10.1074/jbc.M701239200 Pentaketide resorcylic acid synthesis by type III polyketide synthase from Neurospora crassa

상세보기
Chem. Biol. Rubin-Pitel 15 1079 2008 10.1016/j.chembiol.2008.08.011 Distinct structural elements dictate the specificity of the type III pentaketide synthase from Neurospora crassa

상세보기
Bioorg. Med. Chem. Lett. Li 21 6085 2011 10.1016/j.bmcl.2011.08.058 Cloning and characterization of a type III polyketide synthase from Aspergillus niger

상세보기
IUBMB Life Yu 64 285 2012 10.1002/iub.1005 Type III polyketide synthases in natural product biosynthesis

상세보기
Mol. Biosyst. Jeya 8 2864 2012 10.1039/c2mb25282a The Botrytis cinerea type III polyketide synthase shows unprecedented high catalytic efficiency toward long chain acyl-CoAs

상세보기
Nat. Prod. Rep. Hashimoto 31 1306 2014 10.1039/C4NP00096J Fungal type III polyketide synthases

상세보기
Enzyme Microb. Technol. Ramakrishnan 115 16 2018 10.1016/j.enzmictec.2018.04.006 Molecular characterization of two alkylresorcylic acid synthases from Sordariomycetes fungi

상세보기
Virology J. Rose 2 20 2005 10.1186/1743-422X-2-20 CODEHOP-mediated PCR - A powerful technique for the identification and characterization of viral genomes

상세보기
Curr. Sci. Kharwar 95 2 228 2008 The endophytic fungal complex of Catharanthus roseus (L.) G. Don
Braz. Arch. Biol. Technol. Katoch 57 714 2014 10.1590/S1516-8913201402502 Endophytic fungi found in association with Bacopa monnieri as potential producers of industrial enzymes and antimicrobial bioactive compounds

상세보기
White 315 1990 PCR Protocols: A Guide to Methods and Applications 38 - amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics
Appl. Microbiol. Biotechnol. Jeya 86 5 1473 2010 10.1007/s00253-009-2395-8 Characterization of β-glucosidase from a strain of Penicillium purpurogenum KJS506

상세보기
Mycol. Res. Schulz 106 996 2002 10.1017/S0953756202006342 Endophytic fungi: a source of novel biologically active secondary metabolites

상세보기
Microbiol. Mol. Biol. Rev. Strobel 67 491 2003 10.1128/MMBR.67.4.491-502.2003 Bioprospecting for microbial endophytes and their natural products

상세보기
J. Nat. Prod. Gunatilaka 69 509 2006 10.1021/np058128n Natural products from plant-associated microorganisms: distribution, structural diversity, bioactivity, and implications of their occurrence

상세보기
Nucleic Acids Res. Jabado 34 6605 2006 10.1093/nar/gkl966 Greene SCPrimer: a rapid comprehensive tool for designing degenerate primers from multiple sequence alignments

상세보기
Microb. Ecol. Rojas 63 565 2012 10.1007/s00248-011-9938-0 The diversity of polyketide synthase genes from sugarcane-derived fungi

상세보기
PLoS One Miller 7 e35953 2012 10.1371/journal.pone.0035953 Investigation of the biosynthetic potential of endophytes in traditional Chinese anticancer herbs

상세보기
Fungal Biol. Muggia 114 4 379 2010 10.1016/j.funbio.2010.03.001 Erratum type III polyketide synthases in lichen mycobionts [Lucia MUGGIA, Martin GRUBE

상세보기
Nat. Toxins Evidente 2 4 1994 10.1002/nt.2620020103 Fusapyrone and deoxyfusapyrone, two antifungal alpha-pyrones from Fusarium semitectum

상세보기
Chem. Rev. Kozubek 99 1 1999 10.1021/cr970464o Resorcinolic lipids, the natural non-isoprenoid phenolic amphiphiles and their biological activity

상세보기

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Identification and characterization of type III polyketide synthase genes from culturable endophytes of ethnomedicinal plants

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Identification and characterization of type III polyketide synthase genes from culturable endophytes of ethnomedicinal plants

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