[논문]Goat milk with and without increased concentrations of lysozyme improves repair of intestinal cell damage induced by enteroaggregative Escherichia coli

Carvalho, Eunice B; Maga, Elizabeth A; Quetz, Josiane S; Lima, Ila FN; Magalhã; es, Hemerson YF; Rodrigues, Felipe AR; Silva, Antô; nio VA; Prata, Mara MG; Cavalcante, Paloma A; Havt, Alexandre; Bertolini, Marcelo; Bertolini, Luciana R; Lima, Aldo AM

doi:10.1186/1471-230X-12-106

Goat milk with and without increased concentrations of lysozyme improves repair of intestinal cell damage induced by enteroaggregative Escherichia coli 원문보기

BMC gastroenterology, v.12, 2012년, pp.106 - 106

Carvalho, Eunice B (Department of Physiology and Pharmacology & INCT-Biomedicine, Faculty of Medicine, Federal University of Ceará) , Maga, Elizabeth A (, Fortaleza, Ceará) , Quetz, Josiane S (, Brazil) , Lima, Ila FN (Department of Animal Science, University of California, Davis, CA, USA) , Magalhães, Hemerson YF (Department of Physiology and Pharmacology & INCT-Biomedicine, Faculty of Medicine, Federal University of Ceará) , Rodrigues, Felipe AR (, Fortaleza, Ceará) , Silva, Antônio VA (, Brazil) , Prata, Mara MG (Department of Physiology and Pharmacology & INCT-Biomedicine, Faculty of Medicine, Federal University of Ceará) , Cavalcante, Paloma A (, Fortaleza, Ceará) , Havt, Alexandre (, Brazil) , Bertolini, Marcelo (Department of Physiology and Pharmacology & INCT-Biomedicine, Faculty of Medicine, Federal University of Ceará) , Bertolini, Luciana R (, Fortaleza, Ceará) , Lima, Aldo AM (, Brazil)

Abstract ▼ AI-Helper

BackgroundEnteroaggregative Escherichia coli (EAEC) causes diarrhea, malnutrition and poor growth in children. Human breast milk decreases disease-causing bacteria by supplying nutrients and antimicrobial factors such as lysozyme. Goat milk with and without human lysozyme (HLZ) may improve the repair of intestinal barrier function damage induced by EAEC. This work investigates the effect of the milks on intestinal barrier function repair, bacterial adherence in Caco-2 and HEp-2 cells, intestinal cell proliferation, migration, viability and apoptosis in IEC-6 cells in the absence or presence of EAEC.MethodsRat intestinal epithelial cells (IEC-6, ATCC, Rockville, MD) were used for proliferation, migration and viability assays and human colon adenocarcinoma (Caco-2, ATCC, Rockville, MD) and human larynx carcinoma (HEp-2, ATCC, Rockville, MD) cells were used for bacterial adhesion assays. Goats expressing HLZ in their milk were generated and express HLZ in milk at concentration of 270 μg/ml . Cells were incubated with pasteurized milk from either transgenic goats expressing HLZ or non-transgenic control goats in the presence and absence of EAEC strain 042 (O44:H18).ResultsCellular proliferation was significantly greater in the presence of both HLZ transgenic and control goat milk compared to cells with no milk. Cellular migration was significantly decreased in the presence of EAEC alone but was restored in the presence of milk. Milk from HLZ transgenic goats had significantly more migration compared to control milk. Both milks significantly reduced EAEC adhesion to Caco-2 cells and transgenic milk resulted in less colonization than control milk using a HEp-2 assay. Both milks had significantly increased cellular viability as well as less apoptosis in both the absence and presence of EAEC.ConclusionsThese data demonstrated that goat milk is able to repair intestinal barrier function damage induced by EAEC and that goat milk with a higher concentration of lysozyme offers additional protection.

주제어

참고문헌 (42)

Viswanathan VK Hodges K Hecht G Enteric infection meets intestinal function: how bacterial pathogens cause diarrhoea Nat Rev Microbiol 2009 7 110 119 19116615

상세보기
Bjerknes M Cheng H Gastrointestinal stem cells II. Intestinal stem cells. Am J Physiol Gastrointest Liver Physiol 2005 289 3 G381 G387
Guerrant RL Oria RB Moore SR Oria MO Lima AA Malnutrition as an enteric infectious disease with long-term effects on child development Nutr Rev 2008 66 487 505 10.1111/j.1753-4887.2008.00082.x 18752473

상세보기
Ziegler TR Evans ME Fernández-Estívariz C Jones DP Trophic and cytoprotective nutrition for intestinal adaptation, mucosal repair, and barrier function Ann Rev Nutr 2003 23 229 261 10.1146/annurev.nutr.23.011702.073036 12626687

상세보기
Lima AAM Moore SR Barboza MS Soares AM Schleupner MA Newman RD Sears CL Nataro JP Fedorko DP Wuhib T Schorling JB Guerrant RL Persistent diarrhea signals a critical period of increased diarrhea burdens and nutritional shortfalls: a prospective cohort study among children in northeastern Brazil J Infect Dis 2000 181 1643 165 10.1086/315423 10823764

상세보기
Moore SR Lima NL Soares AM Oriá RB Pinkerton RC Barrett LJ Guerrant RL Lima AA Prolonged episodes of acute diarrhea reduce growth and increase risk of persistent diarrhea in children Gastroenterology 2010 139 1156 1164 10.1053/j.gastro.2010.05.076 20638937

상세보기
Petri WA Miller M, Binder HJ, Levine MM, Dillingham R, Guerrant RL: Enteric infections, diarrhea, and their impact on function and development J Clin Invest 2008 118 1277 1290 10.1172/JCI34005 18382740

상세보기
Huang DB Nataro JP DuPont HL Kamat PP Mhatre AD Okhuysen P Chiang T Enteroaggregative Escherichia coli is a cause of acute diarrheal illness: a meta-analysis Clin Infect Dis 2006 43 556 563 10.1086/505869 16886146

상세보기
Rasko DA Webster DR Sahl JW Bashir A Boisen N Scheutz F Paxinos EE Sebra R Chin CS Iliopoulos D Klammer A Peluso P Lee L Kislyuk AO Bullard J Kasarskis A Wang S Eid J Rank D Redman JC Steyert SR Frimodt-Møller J Struve C Petersen AM Krogfelt KA Nataro JP Schadt EE Waldor MK Origins of the E. coli strain causing an outbreak of hemolytic-uremic syndrome in Germany N Engl J Med 2011 365 709 717 10.1056/NEJMoa1106920 21793740

상세보기
Steiner TS Lima AA M Nataro JP Guerrant RL Lima AA M, Nataro JP, Guerrant RL: Enteroaggregative Escherichia coli produce intestinal inflammation and growth impairment and cause interleukin-8 release from intestinal epithelial cells J Infect Dis 1998 177 88 96 10.1086/513809 9419174

상세보기
Harrington SM Strauman MC Abe CM Nataro P Aggregative adherence fimbriae contribute to the inflammatory response of epithelial cells infected with enteroaggregative Escherichia coli Cell Microbiol 2005 7 1565 1578 10.1111/j.1462-5822.2005.00588.x 16207244

상세보기
Steiner TS Nataro JP Poteet-Smith CE Smith JA Guerrant RL Enteroaggregative Escherichia coli expresses a novel flagellin that causes IL-8 release from intestinal epithelial cells J Clin Invest 2000 105 1769 1777 10.1172/JCI8892 10862792

상세보기
Goldman AS The immune system in human milk and the developing infant Breastfeed Med 2007 2 195 204 10.1089/bfm.2007.0024 18081456

상세보기
Lonnerdal B Nutritional and physiologic significance of human milk proteins Am J Clin Nutr 2003 77 Suppl 6 1537 1543
Park YM Juárez M Ramos M Haenlein GFW Physico-chemical characteristics of goat and sheep milk Small Rumin Res 2007 68 88 113 10.1016/j.smallrumres.2006.09.013

상세보기
Chandan RC Parry RM Shahani KM Lysozyme, lipase, and ribonuclease in milk of various species J Dairy Sci 1968 51 606 607 10.3168/jds.S0022-0302(68)87036-5

상세보기
Maga EA Sargent RG Zeng H Pati S Zarling DA Oppenheim SM Collette NM Moyer AL Conrad-Brink JS Rowe JD Bondurant RH Anderson GB Murray JD Increased efficiency of transgenic livestock production Transgenic Res 2003 12 485 496 10.1023/A:1024257906647 12885169

상세보기
Maga EA Shoemaker CF Rowe JD Bondurant RH Anderson GB Murray JD Production and processing of milk from transgenic goats expressing human lysozyme in the mammary gland J Dairy Sci 2006 89 518 524 10.3168/jds.S0022-0302(06)72114-2 16428620

상세보기
Maga EA Cullor JS Smith W Anderson GB Murray JD Human lysozyme expressed in the mammary gland of transgenic dairy goats can inhibit the growth of bacteria that cause mastitis and the cold-spoilage of milk Foodborne Pathog Dis 2006 3 384 392 10.1089/fpd.2006.3.384 17199520

상세보기
Maga EA Walker RL Anderson G B, Murray JD: Consumption of milk from transgenic goats expressing human lysozyme in the mammary gland results in the modulation of intestinal microflora Transgenic Res 2006 15 515 519 10.1007/s11248-006-0014-3 16906451

상세보기
Brundige DR Maga EA Klasing KC Murray JD Lysozyme transgenic goats’ milk influences gastrointestinal morphology in young pigs J Nutr 2008 138 921 926 18424602

상세보기
Cooper CA Brundige DR Reh WA Maga EA Murray JD Lysozyme transgenic goats’ milk positively impacts intestinal cytokine expression and morphology Transgenic Res 2011 20 1235 1243 10.1007/s11248-011-9489-7 21311970

상세보기
McComarck SA Viar MJ Johnson LR Migration of IEC-6: a model for mucosal healing Am J Physiol 1992 263 G426 G435 1415554
Mariadason JM Rickard KL Barkla DH Augenlicht LH Gibson PR Divergent phenotypic patterns and commitment to apoptosis of Caco-2 cells during spontaneous and butyrate-induced differentiation J Cell Physiol 2000 183 347 354 10.1002/(SICI)1097-4652(200006)183:3 3.0.CO;2-W 10797309

상세보기
Nataro JP Deng Y Maneval DR German AL Martin WC Levine MM Aggregative adherence fimbriae I of enteroaggregative Escherichia coli mediate adherence to HEp-2 cells and hemagglutination of human erythrocytes Infect Immun 1992 60 2297 2304 1350273
Nataro JP Deng Y Cookson S Cravioto A Savarino SJ Guers LD Levine MM Tacket CO Heterogeneity of enteroaggregative Escherichia coli virulence demonstrated in volunteers J Infect Dis 1995 171 465 468 10.1093/infdis/171.2.465 7844392

상세보기
Brito GA Carneiro-Filho B Oriá RB Destura RV Lima AA Guerrant RL Clostridium difficile toxin A induces intestinal epithelial cell apoptosis and damage: role of Gln and Ala-Gln in toxin A effects Dig Dis Sci 2005 50 1271 1278 10.1007/s10620-005-2771-x 16047471

상세보기
Roselli M Finamore A Garaguso I Britti MA Mengheri E Zinc oxide protects cultured enterocytes from the damage induced by Escherichia coli J Nutr 2003 133 4077 4082 14652351

상세보기
Kosek M Bern C Guerrant RL The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000 Bull World Health Organ 2003 81 197 204 12764516

상세보기
Bhandari N Mazumder S Taneja S Dube B Agarwal RC Mahalanabis D Fontaine O Black RE Bhan MK Effectiveness of zinc supplementation plus oral rehydration salts compared with oral rehydration salts alone as a treatment for acute diarrhea in a primary care setting: a cluster randomized trial Pediatrics 2008 121 e1279 1285 10.1542/peds.2007-1939 18450870

상세보기
Bushen OY Davenport JA Lima AB Piscitelli SC Uzgiris AJ Silva TM Leite R Kosek M Dillingham RA Girao A Lima AA Guerrant RL Diarrhea and reduced levels of antiretroviral drugs: improvement with glutamine or alanyl-glutamine in a randomized controlled trial in northeast Brazil Clin Infect Dis 2004 38 1764 1770 10.1086/421394 15227625

상세보기
Lazzerini M Ronfani L Oral zinc for treating diarrhoea in children Cochrane Database Syst Rev 2008 3 005436
Lima NL Soares AM Mota RM Monteiro HS Guerrant RL Lima AA Wasting and intestinal barrier function in children taking alanyl-glutamine-supplemented enteral formula J Pediatr Gastroenterol Nutr 2007 44 365 374 10.1097/MPG.0b013e31802eecdd 17325559

상세보기
Kono H Fujii H Ogiku M Tsuchiya M Ishii K Hara M Enteral diets enriched with medium-chain triglycerides and N-3 fatty acids prevent chemically induced experimental colitis in rats Transl Res 2010 156 282 291 10.1016/j.trsl.2010.07.012 20970751

상세보기
Hermans D Martel A Van Deun K Verlinden M Van Immerseel F Gar Martel A Van Deun K Verlinden M Van Immerseel F Garmyn A Messens W Heyndrickx M Haesebrouck F Pasmans F Gar Martel A, Van Deun K, Verlinden M, Van Immerseel F, Garmyn A, Messens W, Heyndrickx M, Haesebrouck F, Pasmans F, Intestinal mucus protects Campylobacter jejuni in the ceca of colonized broiler chickens against the bactericidal effects of medium-chain fatty acids Poult Sci 2010 89 1144 1155 10.3382/ps.2010-00717 20460660

상세보기
Van Immerseel F De Buck J Boyen F Bohez L Pasmans F Volf J Sevcik M Rychlik I Haesebrouck F Ducatelle R Medium-chain fatty acids decrease colonization and invasion through hilA suppression shortly after infection of chickens with Salmonella enterica serovar Enteritidis Appl Environ Microbiol 2004 70 3582 3587 10.1128/AEM.70.6.3582-3587.2004 15184160

상세보기
Maga EA Desai PT Weimer BC Dao N Kültz D Murray JD Consumption of lysozyme-rich milk can alter microbial fecal populations 2012 Appl Environ Microbiol, Appl Environ Microbiol
Lu L Walker WA Pathologic and physiologic interactions of bacteria with the gastrointestinal epithelium Am J Clin Nutr 2001 73 Suppl 6 1124 1130
Nataro JP Enteroaggregative Escherichia coli pathogenesis Curr Opin Gastroenterol 2005 21 4 8 15687877

상세보기
Huang DB Mohamed JA Nataro JP Dupont HL Jiang ZD Okhuysen PC Virulence characteristics and the molecular epidemiology of enteroaggregative Escherichia coli isolates from travelers to developing countries J Med Microbiol 2007 56 1386 1392 10.1099/jmm.0.47161-0 17893178

상세보기
Shoji H Oguchi S Fujinaga S Shinohara K Kaneko K Shimizu T Yamashiro Y Effects of human milk and spermine on hydrogen peroxide-induced oxidative damage in IEC-6 cells J Pediatr Gastroenterol Nutr 2005 41 460 465 10.1097/01.mpg.0000176180.89261.bf 16205515

상세보기
Capano G Bloch KJ Carter EA Dascoli JA Schoenfeld D Harmatz PR Polyamines in human and rat milk influence intestinal cell growth in vitro J Pediatr Gastroenterol Nutr 1998 27 281 286 10.1097/00005176-199809000-00002 9740197

상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Goat milk with and without increased concentrations of lysozyme improves repair of intestinal cell damage induced by enteroaggregative Escherichia coli 원문보기

Abstract ▼ AI-Helper

주제어

참고문헌 (42)

이 논문을 인용한 문헌

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트