[논문]Engineering Biology to Construct Microbial Chassis for the Production of Difficult-to-Express Proteins

Kim, Kangsan; Choe, Donghui; Lee, Dae-Hee; Cho, Byung-Kwan

doi:10.3390/ijms21030990

[해외논문] Engineering Biology to Construct Microbial Chassis for the Production of Difficult-to-Express Proteins 원문보기

International journal of molecular sciences, v.21 no.3, 2020년, pp.990 -

Kim, Kangsan (Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea) , Choe, Donghui (kskim2474@kaist.ac.kr (K.K.)) , Lee, Dae-Hee (robinald@kaist.ac.kr (D.C.)) , Cho, Byung-Kwan (Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea)

Abstract ▼ AI-Helper

A large proportion of the recombinant proteins manufactured today rely on microbe-based expression systems owing to their relatively simple and cost-effective production schemes. However, several issues in microbial protein expression, including formation of insoluble aggregates, low protein yield, and cell death are still highly recursive and tricky to optimize. These obstacles are usually rooted in the metabolic capacity of the expression host, limitation of cellular translational machineries, or genetic instability. To this end, several microbial strains having precisely designed genomes have been suggested as a way around the recurrent problems in recombinant protein expression. Already, a growing number of prokaryotic chassis strains have been genome-streamlined to attain superior cellular fitness, recombinant protein yield, and stability of the exogenous expression pathways. In this review, we outline challenges associated with heterologous protein expression, some examples of microbial chassis engineered for the production of recombinant proteins, and emerging tools to optimize the expression of heterologous proteins. In particular, we discuss the synthetic biology approaches to design and build and test genome-reduced microbial chassis that carry desirable characteristics for heterologous protein expression.

Keyword

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저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

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