[논문]Boosting productivity of heterotrophic microalgae by efficient control of the oxygen transfer coefficient using a microbubble sparger

Ju, Jung-Hyun; Oh, Beak-Rock; Ko, Dong-Jin; Heo, Sun-Yeon; Lee, Jong-Jea; Kim, Young-Min; Yang, KwonSeok; Seo, Jeong-Woo; Hong, Won-Kyung; Kim, Chul-Ho

doi:10.1016/j.algal.2019.101474

Boosting productivity of heterotrophic microalgae by efficient control of the oxygen transfer coefficient using a microbubble sparger

Algal research, v.41, 2019년, pp.101474 -

Ju, Jung-Hyun (Microbial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) , Oh, Beak-Rock (Microbial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) , Ko, Dong-Jin (Microbial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) , Heo, Sun-Yeon (Microbial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) , Lee, Jong-Jea (Microbial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) , Kim, Young-Min (Department of Food Science & Technology and Functional Food Research Center, Chonnam National University) , Yang, KwonSeok (A) , Seo, Jeong-Woo , Hong, Won-Kyung , Kim, Chul-Ho

Abstract ▼ AI-Helper

Abstract We isolated and characterized an oleaginous Thraustochytrid microalga. Based on 18 s rRNA sequencing, we classified this strain as Schizochytrium sp. SH103. Growth of these cells in a 5 L fermentor with a microbubble-type sparger (MTS) efficiently increased the oxygen transfer coefficient (OTC), while inducing only low external stress. For fermentation under conditions of low agitation (100 rpm) and aeration (3 L/min), the OTC was 8.6-fold higher using a MTS than a general type sparger (GTS). Under these same conditions, dry cell weight (DCW) productivity (0.54 vs. 1.26 g/L·h) and total lipid productivity (0.29 vs. 0.61 g/L·h) were each about 2.2-fold higher using a MTS than a GTS. Under conditions that optimized the OTC in a MTS fermentor (876 h−1), DCW productivity, total lipid productivity and DHA productivity were each 1.3-fold greater than non-optimized condition. Fed-batch fermentation experiments indicated the maximal DCW was 100.6 g/L at 48 h, maximal DCW productivity was 2.1 g/L·h, maximal lipid content was 48.6 g/L at 48 h, and lipid productivity was 1.0 g/L·h. Additionally, the results of experiments using a 50 L MTS fermentor (96.0 g/L DCW and 44.3 g/L total lipids at 48 h) were similar to those using the 5 L fermentor. Our results suggest that use of a MTS may help to achieve the efficient fermentation of heterotrophic microalgae for high cell densities. Highlights The oxygen transfer coefficient is a key parameter in heterotrophic microalgae fermentation. The microbubble-type sparger can efficiently increase the oxygen transfer coefficient. The strategy of microbubble fermentation efficiently improved biomass and bio-oil productivity. Graphical abstract The oxygen transfer coefficient (OTC) is key parameter of high cell density fermentation in heterotrophic microalgae. The microbubble fermentation could increase the OTC with low agitation speed and low aeration rate. Biomass and bio-oil production readily achieved high concentration and high productivity using the microbubble in heterotrophic microalgae fermentation. The results got in our study have important significance in heterotrophic microalgae fermentation. [DISPLAY OMISSION]

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Boosting productivity of heterotrophic microalgae by efficient control of the oxygen transfer coefficient using a microbubble sparger

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Boosting productivity of heterotrophic microalgae by efficient control of the oxygen transfer coefficient using a microbubble sparger

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