[논문]Novel Dental Poly (Methyl Methacrylate) Containing Phytoncide for Antifungal Effect and Inhibition of Oral Multispecies Biofilm

Lee, Myung-Jin; Kim, Min-Ji; Oh, Sang-Hwan; Kwon, Jae-Sung

doi:10.3390/ma13020371

Novel Dental Poly (Methyl Methacrylate) Containing Phytoncide for Antifungal Effect and Inhibition of Oral Multispecies Biofilm 원문보기

Materials, v.13 no.2, 2020년, pp.371 -

Lee, Myung-Jin (Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul 03722, Korea) , Kim, Min-Ji (lmj239@yuhs.ac (M.-J.L.)) , Oh, Sang-Hwan (dmmj@yuhs.ac (M.-J.K.)) , Kwon, Jae-Sung (Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul 03722, Korea)

Abstract ▼ AI-Helper

Despite the many advantages of poly (methyl methacrylate) (PMMA) as a dental polymer, its antifungal and antibacterial effects remain limited. Here, phytoncide was incorporated into PMMA to inhibit fungal and biofilm accumulation without impairing the basic and biological properties of PMMA. A variable amount of phytoncide (0 wt % to 5 wt %) was incorporated into PMMA, and the basic material properties of microhardness, flexural strength and gloss were evaluated. In addition, cell viability was confirmed by MTT assay. This MTT assay measures cell viability via metabolic activity, and the color intensity of the formazan correlates viable cells. The fungal adhesion and viability on the PMMA surfaces were evaluated using Candida albicans (a pathogenic yeast). Finally, the thickness of saliva-derived biofilm was estimated. The flexural strength of PMMA decreased with increasing phytoncide contents, whereas there were no significant differences in the microhardness and gloss (p > 0.05) and the cell viability (p > 0.05) between the control and the phytoncide-incorporated PMMA samples. The amounts of adherent Candida albicans colony-forming unit (CFU) counts, and saliva-derived biofilm thickness were significantly lower in the phytoncide-incorporated PMMA compared to the control (p < 0.05). Hence, it was concluded that the incorporation of appropriate amounts of phytoncide in PMMA demonstrated antifungal effects while maintaining the properties, which could be a possible use in dentistry application such as denture base resin.

주제어

참고문헌 (31)

1. Vojdani M. Giti R. Polyamide as a Denture Base Material: A Literature Review J. Dent. 2015 16 Suppl. 1 1 9
2. Matsumura K. Sato Y. Kitagawa N. Shichita T. Kawata D. Ishikawa M. Influence of denture surface roughness and host factors on dental calculi formation on dentures: A cross-sectional study BMC Oral Health 2018 18 78 10.1186/s12903-018-0543-1 29728082

상세보기
3. Bacali C. Badea M. Moldovan M. Sarosi C. Nastase V. Baldea I. Chiorean R.S. Constantiniuc M. The Influence of Graphene in Improvement of Physico-Mechanical Properties in PMMA Denture Base Resins Materials 2019 12 2335 10.3390/ma12142335 31340462

상세보기
4. Soygun K. Bolayir G. Boztug A. Mechanical and thermal properties of polyamide versus reinforced PMMA denture base materials J. Adv. Prosthodont. 2013 5 153 160 10.4047/jap.2013.5.2.153 23755341

원문보기 상세보기
5. Jorge J.H. Giampaolo E.T. Machado A.L. Vergani C.E. Cytotoxicity of denture base acrylic resins: A literature review J. Prosthet. Dent. 2003 90 190 193 10.1016/S0022-3913(03)00349-4 12886213

상세보기
6. Moosazadeh M. Akbari M. Tabrizi R. Ghorbani A. Golkari A. Banakar M. Sekhavati E. Kavari S.H. Bagheri Lankarani L. Denture Stomatitis and Candida Albicans in Iranian Population: A Systematic Review and Meta-Analysis J. Dent. 2016 17 Suppl. 3 283 292
7. Zhang Y. Chen Y.Y. Huang L. Chai Z.G. Shen L.J. Xiao Y.H. The antifungal effects and mechanical properties of silver bromide/cationic polymer nano-composite-modified Poly-methyl methacrylate-based dental resin Sci. Rep. 2017 7 1547 10.1038/s41598-017-01686-4 28484255

상세보기
8. De Freitas Fernandes F.S. Pereira-Cenci T. da Silva W.J. Filho A.P. Straioto F.G. Del Bel Cury A.A. Efficacy of denture cleansers on Candida spp. biofilm formed on polyamide and polymethyl methacrylate resins J. Prosthet. Dent. 2011 105 51 58 21194588

상세보기
9. Park S.E. Periathamby A.R. Loza J.C. Effect of surface-charged poly(methyl methacrylate) on the adhesion of Candida albicans J. Prosthodont. 2003 12 249 254 10.1016/S1059-941X(03)00107-4 15061233

상세보기
10. Acosta-Torres L.S. Mendieta I. Nunez-Anita R.E. Cajero-Juarez M. Castano V.M. Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures Int. J. Nanomed. 2012 7 4777 4786

상세보기
11. Al-Thobity A.M. Al-Khalifa K.S. Gad M.M. Al-Hariri M. Ali A.A. Alnassar T. In Vitro Evaluation of the Inhibitory Activity of Thymoquinone in Combatting Candida albicans in Denture Stomatitis Prevention Int. J. Environ. Res. Public Health 2017 14 743 10.3390/ijerph14070743 28698449

상세보기
12. Alaufi O.M. Noorwali A. Zahran F. Al-Abd A.M. Al-Attas S. Cytotoxicity of thymoquinone alone or in combination with cisplatin (CDDP) against oral squamous cell carcinoma in vitro Sci. Rep. 2017 7 13131 10.1038/s41598-017-13357-5 29030590

상세보기
13. Kim J.E. Kim H.E. Hwang J.K. Lee H.J. Kwon H.K. Kim B.I. Antibacterial characteristics of Curcuma xanthorrhiza extract on Streptococcus mutans biofilm J. Microbiol. 2008 46 228 232 10.1007/s12275-007-0167-7 18545974

원문보기 상세보기
14. Li Q. Kobayashi M. Wakayama Y. Inagaki H. Katsumata M. Hirata Y. Hirata K. Shimizu T. Kawada T. Park B.J. Effect of phytoncide from trees on human natural killer cell function Int. J. Immunopathol. Pharmacol. 2009 22 951 959 10.1177/039463200902200410 20074458

상세보기
15. Kim S.Q. Shin M.K. Auh Q.S. Lee J.Y. Hong J.P. Chun Y.H. Effect of phytoncide on porphyromonas gingivalis J. Oral Med. Pain 2007 32 137 150
16. Kang S.N. Kim S.E. Choi J. Park K. Goo J.H. Sim D.S. Hong Y.J. Kim J.H. Joung Y.K. Lee J. Comparison of phytoncide with sirolimus as a novel drug candidate for drug-eluting stent Biomaterials 2015 44 1 10 10.1016/j.biomaterials.2014.12.015 25617121

상세보기
17. Liu S.Y. Tonggu L. Niu L.N. Gong S.Q. Fan B. Wang L. Zhao J.H. Huang C. Pashley D.H. Tay F.R. Antimicrobial activity of a quaternary ammonium methacryloxy silicate-containing acrylic resin: A randomised clinical trial Sci. Rep. 2016 6 21882 10.1038/srep21882 26903314

상세보기
18. International Organization for Standardization ISO 20795-1 Dentistry―Base Polymers―Part 1: Denture Base Polymers ISO Geneva, Switzerland 2013
19. Lee M.J. Kim M.J. Kwon J.S. Lee S.B. Kim K.M. Cytotoxicity of Light-Cured Dental Materials according to Different Sample Preparation Methods Materials 2017 10 288 10.3390/ma10030288

상세보기
20. Lee M.J. Kwon J.S. Jiang H.B. Choi E.H. Park G. Kim K.M. The antibacterial effect of non-thermal atmospheric pressure plasma treatment of titanium surfaces according to the bacterial wall structure Sci. Rep. 2019 9 1938 10.1038/s41598-019-39414-9 30760871

상세보기
21. Lee M.J. Kwon J.S. Kim J.Y. Ryu J.H. Seo J.Y. Jang S. Kim K.M. Hwang C.J. Choi S.H. Bioactive resin-based composite with surface pre-reacted glass-ionomer filler and zwitterionic material to prevent the formation of multi-species biofilm Dent. Mater. 2019 35 1331 1341 10.1016/j.dental.2019.06.004 31320183

상세보기
22. Cheng L. Zhang K. Melo M.A. Weir M.D. Zhou X. Xu H.H. Anti-biofilm dentin primer with quaternary ammonium and silver nanoparticles J. Dent. Res. 2012 91 598 604 10.1177/0022034512444128 22492276

상세보기
23. McBain A.J. Chapter 4: In vitro biofilm models: An overview Adv. Appl. Microbiol. 2009 69 99 132 19729092

상세보기
24. McBain A.J. Sissons C. Ledder R.G. Sreenivasan P.K. De Vizio W. Gilbert P. Development and characterization of a simple perfused oral microcosm J. Appl. Microbiol. 2005 98 624 634 10.1111/j.1365-2672.2004.02483.x 15715865

상세보기
25. Lee J.H. El-Fiqi A. Jo J.K. Kim D.A. Kim S.C. Jun S.K. Kim H.W. Lee H.H. Development of long-term antimicrobial poly(methyl methacrylate) by incorporating mesoporous silica nanocarriers Dent. Mater. 2016 32 1564 1574 10.1016/j.dental.2016.09.001 27671462

상세보기
26. Wen J. Jiang F. Yeh C.K. Sun Y. Controlling fungal biofilms with functional drug delivery denture biomaterials Colloids Surf. B Bioninterfaces 2016 140 19 27 10.1016/j.colsurfb.2015.12.028

상세보기
27. Zida A. Bamba S. Yacouba A. Ouedraogo-Traore R. Guiguemde R.T. Anti-Candida albicans natural products, sources of new antifungal drugs: A review J. Mycol. Med. 2017 27 1 19 10.1016/j.mycmed.2016.10.002 27842800

상세보기
28. Jung S.H. Auh Q.S. Chun Y.H. Hong J.P. The effect of S. thermophilus Isolated from saliva treated with phytoncide on P. gingivalis J. Oral Med. Pain 2009 34 23 37
29. Lappalainen R. Yli-Urpo A. Seppa L. Wear of dental restorative and prosthetic materials in vitro Dent. Mater. 1989 5 35 37 10.1016/0109-5641(89)90090-0 2606266

상세보기
30. ISO 10993-5 Biological Evaluation Evaluation of Medical Devices-Part 5: Tests for In Vitro Cytotoxicity International Standard Geneva, Switzerland 2009
31. Sumi Y. Miura H. Michiwaki Y. Nagaosa S. Nagaya M. Colonization of dental plaque by respiratory pathogens in dependent elderly Arch. Gerontol. Geriatr. 2007 44 119 124 10.1016/j.archger.2006.04.004 16723159

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Novel Dental Poly (Methyl Methacrylate) Containing Phytoncide for Antifungal Effect and Inhibition of Oral Multispecies Biofilm 원문보기

Abstract ▼ AI-Helper

주제어

참고문헌 (31)

이 논문을 인용한 문헌

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트