[논문]A D-glucose- and D-xylose-tolerant GH1 β-glucosidase from Cellulosimicrobium funkei HY-13, a fibrolytic gut bacterium of Eisenia fetida

Kim, Do Young; Kim, Jonghoon; Lee, Sun Hwa; Chung, Chungwook; Shin, Dong-Ha; Ku, Bon-Hwan; Son, Kwang-Hee; Park, Ho-Yong

doi:10.1016/j.procbio.2020.04.033

[해외논문] A D-glucose- and D-xylose-tolerant GH1 β-glucosidase from Cellulosimicrobium funkei HY-13, a fibrolytic gut bacterium of Eisenia fetida

Process biochemistry, v.94, 2020년, pp.282 - 288

Kim, Do Young (Industrial Bio-materials Research Center, KRIBB) , Kim, Jonghoon (Industrial Bio-materials Research Center, KRIBB) , Lee, Sun Hwa (Industrial Bio-materials Research Center, KRIBB) , Chung, Chungwook (Department of Biological Sciences, Andong National University) , Shin, Dong-Ha (Insect Biotech Co. Ltd.) , Ku, Bon-Hwan (Insect Biotech Co. Ltd.) , Son, Kwang-Hee (Industrial Bio-materials Research Center, KRIBB) , Park, Ho-Yong (Industrial Bio-materials Research Center, KRIBB)

Abstract ▼ AI-Helper

Abstract The GluM gene (1491-bp) coding for a β-glucosidase comprising a single catalytic glycoside hydrolase family 1 domain from an earthworm (Eisenia fetida)-symbiotic bacterium, Cellulosimicrobium funkei HY-13, was cloned and over-expressed in Escherichia coli BL21. The recombinant histidine-tagged enzyme (rGluM: 56 kDa) displayed the highest cleavage activity toward p-nitrophenyl (pNP)-β-D-glucopyranoside at pH 5.0 and 40 °C. The β-glucosidase activity of rGluM was enhanced over 1.8-fold of its original activity in the presence of 1 mM Ca2+, Ni2+, Mn2+, and Co2+ ions, respectively, while it was highly sensitive to 5 mM N-bromosuccinimide and 1 mM Hg2+. The susceptibility of some pNP-sugar derivatives and D-cellobiose to rGluM was evaluated to be in the order of pNP-β-D-glucopyranoside >pNP-β-D-galactopyranoside >D-cellobiose >pNP-β-D-cellobioside >pNP-β-D-mannopyranoside. The k cat/K m values of rGluM toward pNP-β-D-glucopyranoside, pNP-β-D-galactopyranoside, and D-cellobiose were 302.28, 179.73, and 6.40 mM-1 s-1, respectively. At a concentration below 1.0 M, D-galactose was a potent activator of rGluM with β-glucosidase activity enhanced by approximately 160% in a dose-dependent manner. Moreover, the D-glucose (< 400 mM) and D-xylose (≤ 700 mM) stimulation of rGluM suggests that it can be exploited as a potential biocatalyst to generate D-glucose molecules in D-cellobiose degradation. Highlights A GH1 β-glucosidase (GluM) from Cellulosimicrobium funkei HY-13 was characterized. Recombinant GluM was a new D-glucose- and D-xylose-tolerant β-glucosidase. D-Galactose was a potent activator of recombinant GluM with β-glucosidase activity. Its β-glucosidase activity was stimulated by D-galactose in a dose-dependent manner. Graphical abstract [DISPLAY OMISSION]

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참고문헌 (30)

Cell. Mol. Life Sci. Cairns 67 3389 2010 10.1007/s00018-010-0399-2 β-Glucosidases

상세보기
Crit. Rev. Biotechnol. Bhatia 22 375 2002 10.1080/07388550290789568 Microbial β-glucosidases: cloning, properties, and applications

상세보기
J. Sci. Ind. Res. Sukumaran 64 832 2005 Microbial cellulases - production, applications and challenges

상세보기
Bioethanol. Curr. Opin. Chem. Biol. Gray 10 141 2006 10.1016/j.cbpa.2006.02.035

상세보기
J. Ind. Microbiol. Biotechnol. Kumar 35 377 2008 10.1007/s10295-008-0327-8 Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives

상세보기
Bioresour. Technol. Salgado 267 704 2018 10.1016/j.biortech.2018.07.137 Glucose tolerant and glucose stimulated β-glucosidases

상세보기
J. Microbiol. Park 45 394 2007 A culture-based study of the bacterial communities within the guts of nine longicorn beetle species and their exo-enzyme producing properties for degrading xylan and pectin

원문보기 상세보기
Nat. Rev. Microbiol. Brune 12 168 2014 10.1038/nrmicro3182 Symbiotic digestion of lignocellulose in termite guts

상세보기
Asian Pac. J. Trop. Biomed. Krishnan 4 Suppl 1 S16 2014 10.12980/APJTB.4.2014C95 Insect gut microbiome - an unexploited reserve for biotechnological application

상세보기
Biotechnol. Lett. Kim 39 149 2017 10.1007/s10529-016-2228-7 Biocatalytic characterization of an endo-β-1,4-mannanase produced by Paenibacillus sp. strain HY-8

상세보기
Appl. Environ. Microbiol. Brennan 70 3609 2004 10.1128/AEM.70.6.3609-3617.2004 Unusual microbial xylanases from insect guts

상세보기
Appl. Environ. Microbiol. Kim 75 7275 2009 10.1128/AEM.01075-09 Novel GH10 xylanase, with a fibronectin type 3 domain, from Cellulosimicrobium sp. strain HY-13, a bacterium in the gut of Eisenia fetida

상세보기
Bioresour. Technol. Kim 102 9185 2011 10.1016/j.biortech.2011.06.073 Cloning and characterization of a modular GH5 β-1,4-mannanase with high specific activity from the fibrolytic bacterium Cellulosimicrobium sp. strain HY-13

상세보기
Int. J. Biol. Macromol. Kim 106 620 2018 10.1016/j.ijbiomac.2017.08.063 Genetic and functional characterization of a novel GH10 endo-β-1,4-xylanase with a ricin-type β-trefoil domain-like domain from Luteimicrobium xylanilyticum HY-24

상세보기
Antonie van Leeuwenhoek Kim 109 1 2016 10.1007/s10482-015-0604-2 Genetic and functional characterization of an extracellular modular GH6 endo-β-1,4-glucanase from an earthworm symbiont, Cellulosimicrobium funkei HY-13

상세보기
Int. J. Biol. Macromol. Crespim 82 375 2016 10.1016/j.ijbiomac.2015.09.018 A novel cold-adapted and glucose-tolerant GH1 β-glucosidase from Exiguobacterium antarcticum B7

상세보기
Enzyme Microb. Technol. Fan 49 94 2011 10.1016/j.enzmictec.2011.03.001 Gene cloning and characterization of a cold-adapted β-glucosidase belonging to glycosyl hydrolase family 1 from a psychrotolerant bacterium Micrococcus antarcticus

상세보기
L82. J. Microbiol. Sun 56 656 2018 10.1007/s12275-018-8018-2 Overexpression and characterization of a novel cold-adapted and salt-tolerant GH1 β-glucosidase from the marine bacterium Alteromonas sp

상세보기
Bioresour. Technol. Li 123 15 2012 10.1016/j.biortech.2012.07.083 Molecular cloning and characterization of a novel β-glucosidase with high hydrolyzing ability for soybean isoflavone glycosides and glucose-tolerance from soil metagenomics library

상세보기
Enzyme Microb. Technol. Wang 51 319 2012 10.1016/j.enzmictec.2012.07.015 Characterization of a novel thermostable β-glucosidase from a metagenomic library of termite gut

상세보기
Appl. Microbiol. Biotechnol. Yang 99 8903 2015 10.1007/s00253-015-6619-9 Overexpression and characterization of a glucose-tolerant β-glucosidase from T. aotearoense with high specific activity for cellobiose

상세보기
Int. J. Biol. Macromol. Liew 115 1094 2018 10.1016/j.ijbiomac.2018.04.156 Purification and characterization of a novel GH1 beta-glucosidase from Jeotgalibacillus malaysiensis

상세보기
Proc. Biochem. Ramachandran 47 99 2012 10.1016/j.procbio.2011.10.015 Cloning and characterization of a putative β-glucosidase (NfBGL595) from Neosartorya fischeri

상세보기
Int. J. Biol. Macromol. Sathe 102 805 2017 10.1016/j.ijbiomac.2017.04.078 Heterologous expression and biochemical studies of a thermostable glucose tolerant β-glucosidase from Methylococcus capsulatus (bath strain)

상세보기
J. Mol. Catal. B: Enzym. Akram 124 92 2016 10.1016/j.molcatb.2015.12.005 Cloning with kinetic and thermodynamic insight of a novel hyperthermostable β-glucosidase from Thermotoga naphthophila RKU-10T with excellent glucose tolerance

상세보기
J. Agric. Food Chem. Song 59 1954 2011 10.1021/jf1046915 Comparison of three thermostable β-glucosidases for application in the hydrolysis of soybean isoflavone glycosides

상세보기
Curr. Microbiol. Xu 62 833 2011 10.1007/s00284-010-9766-3 Characterization of a glucose-, xylose-, sucrose-, and d-galactose-stimulated β-glucosidase from the alkalophilic bacterium Bacillus halodurans C-125

상세보기
Int. J. Biol. Macromol. Fusco 113 783 2018 10.1016/j.ijbiomac.2018.03.018 Biochemical characterization of a novel thermostable β-glucosidase from Dictyoglomus turgidum

상세보기
J. Mol. Catal. B: Enzym. Souza 106 1 2014 10.1016/j.molcatb.2014.04.007 Gene cloning, expression, and biochemical characterization of a glucose- and xylose-stimulated β-glucosidase from Humicola insolens RP86

상세보기
PLoS ONE Meleiro 12 2017 10.1371/journal.pone.0188254 Engineering the GH1 β-glucosidase from Humicola insolens: insights on the stimulation of activity by glucose and xylose

상세보기

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DOI : 10.1016/j.procbio.2020.04.033
Elsevier : 저널 > 논문

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