[논문]The Gata1  low  murine megakaryocyte–erythroid progenitor cells expand robustly and alter differentiation potential

Shin, Eunju; Jeong, Jong-Gwan; Chung, Hyunmin; Jung, Haiyoung; Park, Charny; Yoon, Suk Ran; Kim, Tae-Don; Lee, Seung Jin; Choi, Inpyo; Noh, Ji-Yoon

doi:10.1016/j.bbrc.2020.04.143

[해외논문] The Gata1 low murine megakaryocyte–erythroid progenitor cells expand robustly and alter differentiation potential

Biochemical and biophysical research communications, v.528 no.1, 2020년, pp.46 - 53

Shin, Eunju (Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology Yuseong-gu) , Jeong, Jong-Gwan (Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology Yuseong-gu) , Chung, Hyunmin (Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology Yuseong-gu) , Jung, Haiyoung (Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology Yuseong-gu) , Park, Charny (Bioinformatics Team, Research Institute, National Cancer Center) , Yoon, Suk Ran (Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology Yuseong-gu) , Kim, Tae-Don (Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology Yuseong-gu) , Lee, Seung Jin (College of Pharmacy, Chungnam National University) , Choi, Inpyo (Immunotherapy Research Cente) , Noh, Ji-Yoon

Abstract ▼ AI-Helper

Abstract GATA1 is a master transcription factor of megakaryopoiesis and erythropoiesis, and loss-of-function mutation can induce accumulation of megakaryocyte–erythroid progenitors (MEPs) in mice and humans. Accordingly, the murine MEP cell line (termed G1ME2 cells) encoding doxycycline (dox)-inducible anti-Gata1 shRNA on Hprt locus has been developed. The cells were CD41+CD71+KIT+, expand under dox, stem cell factor, and thrombopoietin (TPO), and terminally differentiate into erythroid cells or megakaryocytes upon removal of dox. Surprisingly, in this study, these Gata1 low murine MEPs displayed accelerated growth from around 90–100 days after cell culture, impeded megakaryocytic potential, and maintained erythropoiesis. We specified them as late G1ME2 cells and discovered that increased CD41−KIT+ population during long-term culture was the main reason for the delayed megakaryopoiesis. The CD41 expression level was partially de-repressed by PI3K/AKT inhibitors, suggesting that TPO-mediated cell survival signaling pathway might have impacted on CD41 in the late G1ME2 cells. Nevertheless, among the late cells, the CD41+KIT+ cells could still generate megakaryocytes on dox withdrawal. Taken together, G1ME2 cells could provide a good model to study molecular mechanism of hematopoiesis because of their ability to expand excessively without artificial immortalization. Highlights GATA1 low mouse megakaryocyte-erythroid progenitors expand excessively in vitro. Long-term cultured GATA1 low MEP impeded megakaryopoiesis but retained erythropoiesis. TPO-dependent PI3K/AKT pathway partially involve in CD41 reduction in the MEPs.

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DOI : 10.1016/j.bbrc.2020.04.143
Elsevier : 저널 > 논문

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