[논문]Distinct shear-induced Ca2+ signaling in the left and right atrial myocytes: Role of P2 receptor context

Le, Qui Anh; Kim, Joon-Chul; Kim, Kyeong-Hee; Van Vu, Anh Thi; Woo, Sun-Hee

doi:10.1016/j.yjmcc.2020.04.018

[해외논문] Distinct shear-induced Ca2+ signaling in the left and right atrial myocytes: Role of P2 receptor context

Journal of molecular and cellular cardiology, v.143, 2020년, pp.38 - 50

Le, Qui Anh (College of Pharmacy, Chungnam National University) , Kim, Joon-Chul , Kim, Kyeong-Hee , Van Vu, Anh Thi , Woo, Sun-Hee

Abstract ▼ AI-Helper

Abstract Atrial myocytes are continuously exposed to shear stress during cardiac cycles. Previous reports have shown that shear stress induces two different types of global Ca2+ signaling in atrial myocytes–longitudinal Ca2+ waves (L-waves) and action potential-involved transverse waves (T-waves), and suggested an underlying role of the autocrine activation of P2 receptors. We explored the correlations between ATP release and Ca2+ wave generation in atrial myocytes and investigated why the cells develop two Ca2+-wave types during the same shear force. We examined whether ATP release correlates with different shear-stress (~16 dyn/cm2)-mediated Ca2+ signaling by simultaneous measurement of local Ca2+ and ATP release in individual atrial myocytes using two-dimensional confocal imaging and sniffer patch techniques, respectively. Functional P2X7-receptor-expressing HEK293 cells were established as sniffer cells, which generated currents in real time in response to ATP released from a closely positioned atrial myocyte. Both shear-stress-induced L- and T-waves were preceded by sniffer currents with no difference in the current magnitude. Left atrial (LA) myocytes had two- to three-fold larger sniffer currents than right atrial (RA) cells, as was confirmed by ATP chemiluminescence assay. Shear-stress-induced ATP release was eliminated by connexin (Cx) 43 hemichannel inhibition using La3+, Gap19, or knock-down of Cx43 expression. The level of phosphorylated Cx43 at Ser386 (p-Cx43Ser368), but not total Cx43, was higher in LA versus RA myocytes. Most LA cells (~70%) developed L-waves, whereas most RA myocytes (~80%) presented T-waves. Shear-stress-induced T-waves were completely removed by inhibition of P2X4R, which were most abundant in rat atrial cells. Expression of P2X4R was higher in RA than LA myocytes, whereas expression of P2Y1R, the mediator of L-waves, was higher in LA than RA myocytes. ATP release mainly triggers L-waves in LA myocytes and T-waves in RA myocytes under the same shear force, partly because of the differential expression of P2Y1R and P2X4R between LA and RA myocytes. Higher ATP release in LA myocytes under shear stress may not contribute to determination of the wave pattern. Highlights Atrial myocytes are exposed to fluid shear stress during cardiac cycles. Shear stress induces longitudinal (L) or action-potential-involved (T) Ca2+ waves. “Sniffer patch” with Ca2+ imaging revealed that ATP release precedes these waves. Left atrial cells with higher P2Y1 receptor levels had more L-waves and ATP release. P2X4 receptor mediates T-waves and is more abundant in right atrium vs left atrium.

Keyword

참고문헌 (61)

Physiol. Rev. Lakatta 73 413 1993 10.1152/physrev.1993.73.2.413 Cardiovascular regulatory mechanisms in advanced age

상세보기
Cardiovasc. Res. Nazir 32 52 1996 10.1016/S0008-6363(96)00054-5 Mechanoelectric feedback and atrial arrhythmias

상세보기
Circ. Res. LeGrice 77 182 1995 10.1161/01.RES.77.1.182 Transverse shear along myocardial cleavage planes provides a mechanism for normal systolic wall thickening

상세보기
Am. J. Phys. Costa 276 H595 1999 Laminar fiber architecture and three-dimensional systolic mechanics in canine ventricular myocardium
Cell Biochem. Biophys. Lorenzen-Schmidt 46 113 2006 10.1385/CBB:46:2:113 Chronotropic response of cultured neonatal rat ventricular myocytes to short-term fluid shear

상세보기
Sci. Rep. Gulan 9 100 2019 10.1038/s41598-018-36614-7 Hemodynamic changes in the right ventricle induced by variations of cardiac output: A possible mechanism for arrhythmia occurrence in the outflow tract

상세보기
Crit. Care Nurs. Clin. North Am. Paul 15 407 2003 10.1016/S0899-5885(02)00089-8 Ventricular remodeling

상세보기
Am. Heart J. Goldsmith 140 777 2000 10.1067/mhj.2000.110284 Atrial endocardial changes in mitral valve disease: A scanning electron microscopy study

상세보기
Saffitz 232 2009 Essentials of Rubin’s Pathology Acquired valvular and endocardial diseases
Arch. Biochem. Biophys. Kim 659 33 2018 10.1016/j.abb.2018.09.026 Regulation of cardiac calcium by mechanotransduction: Role of mitochondria

상세보기
Cell Calcium Woo 41 397 2007 10.1016/j.ceca.2006.09.005 Modulation of local Ca2+ release sites by rapid fluid puffing in rat atrial myocytes

상세보기
Biochim. Biophys. Acta Mol. Cell Res. Kim 2017 1121 1864 Shear stress enhances Ca2+ sparks through Nox2-dependent mitochondrial reactive oxygen species generation in rat ventricular myocytes
J. Physiol. Kim 593 5091 2015 10.1113/JP271016 Shear stress induces a longitudinal Ca2+ wave via autocrine activation of P2Y1 purinergic signalling in rat atrial myocytes

상세보기
Cell. Physiol. Biochem. Kim 50 2296 2018 10.1159/000495089 Ca2+ signaling triggered by shear-autocrine P2X receptor pathway in rat atrial myocytes

상세보기
J. Cell Biol. Carl 129 673 1995 10.1083/jcb.129.3.673 Immunolocalization of sarcolemmaldihydropyridine receptor and sarcoplasmic reticular triadin and ryanodine receptor in rabbit ventricle and atrium

상세보기
J. Anat. Ayettey 127 125 1978 The T-tubule system in the specialized and general myocardium of the rat
Trends Neurosci. Allen 20 192 1997 10.1016/S0166-2236(96)01039-9 The ‘sniffer-patch’ technique for detection of neurotransmitter release

상세보기
J. Physiol. Woo 543 439 2002 10.1113/jphysiol.2002.024190 Ca2+ current-gated focal and local Ca2+ release in rat atrial myocytes: Evidence from rapid 2-D confocal imaging

상세보기
Biochem. J. Kunapuli 336 Pt 3 513 1998 10.1042/bj3360513 P2 receptor subtypes in the cardiovascular system

상세보기
Physiol. Rev. North 82 1013 2002 10.1152/physrev.00015.2002 Molecular physiology of P2X receptors

상세보기
J. Physiol. Sartiani 545 81 2002 10.1113/jphysiol.2002.021535 Functional expression of the hyperpolarization-activated non-selective cation current if in immortalized Hl-1 cardiomyocytes

상세보기
Am. J. Physiol. Heart Circ. Physiol. White 286 H823 2004 10.1152/ajpheart.00986.2003 Cardiac physiology at the cellular level: Use of cultured HL-1 cardiomyocytes for studies of cardiac muscle cell structure and function

상세보기
Prog. Biophys. Mol. Biol. Kim 103 59 2010 10.1016/j.pbiomolbio.2010.02.002 Atrial local Ca2+ signaling and inositol 1,4,5-trisphosphate receptors

상세보기
Biochem. Biophys. Acta Solan 1711 154 2005 10.1016/j.bbamem.2004.09.013 Connexin phosphorylation as a regulatory event linked to gap junction channel assembly

상세보기
Cardiovasc. Res. Severs 80 9 2008 10.1093/cvr/cvn133 Remodelling of gap junctions and connexin expression in diseased myocardium

상세보기
Cell Biol. Int. Oviedo-Orta 26 253 2002 10.1006/cbir.2001.0840 Gap junction intercellular communication during lymphocyte transendothelial migration

상세보기
Basic Res. Cardiol. Wang 108 309 2013 10.1007/s00395-012-0309-x Selective inhibition of Cx43 hemichannels by Gap19 and its impact on myocardial ischemia/reperfusion injury

상세보기
Front. Cell. Neurosci. Abudara 8 306 2014 10.3389/fncel.2014.00306 The connexin43 mimetic peptide Gap19 inhibits hemichannels without altering gap junction communication in astrocytes

상세보기
Anal. Biochem. Carrigan 341 290 2005 10.1016/j.ab.2005.03.026 The engineering of membrane-permeable peptides

상세보기
J. Cell Biol. Lampe 126 1503 2000 10.1083/jcb.149.7.1503 Phosphorylation of connexin43 on serine368 by protein kinase C regulates gap junctional communication

상세보기
Biochim. Biophys. Acta Marquez-Rosado 1818 1985 2012 10.1016/j.bbamem.2011.07.028 Connexin43 phosphorylation in brain, cardiac, endothelial and epithelial tissues

상세보기
Circ. Res. Jain 92 1138 2003 10.1161/01.RES.0000074883.66422.C5 Mechanisms of delayed electrical uncoupling induced by ischemic preconditioning

상세보기
J. Med. Chem. Hernandez-Olmos 55 9576 2012 10.1021/jm300845v N-substituted phenoxazine and acridone derivatives: Structure-activity relationships of potent P2X4 receptor antagonists

상세보기
Cell. Physiol. Biochem. Balazs 32 11 2013 10.1159/000350119 Investigation of the inhibitory effects of the benzodiazepine derivative, 5-BDBD on P2X4 purinergic receptors by two complementary methods

상세보기
Front. Pharmacol. Stokes 8 291 2017 10.3389/fphar.2017.00291 P2X4 receptor function in the nervous system and current breakthroughs in pharmacology

상세보기
Annu. Rev. Physiol. Schmid 81 43 2019 10.1146/annurev-physiol-020518-114259 ATP-gated P2X receptor channels: Molecular insights into functional roles

상세보기
J. Physiol. Belmonte 586 1379 2008 10.1113/jphysiol.2007.149294 ‘Pressure-flow’-triggered intracellular Ca2+ transients in rat cardiac myocytes: Possible mechanisms and role of mitochondria

상세보기
Biochem. J. Solan 419 261 2009 10.1042/BJ20082319 Connexin 43 phosphorylation-structural changes and biological effects

상세보기
Mol. Biol. Cell Rhett 22 1516 2011 10.1091/mbc.e10-06-0548 Connexin 43 connexon to gap junction transition is regulated by zonula occludens-1

상세보기
Mol. Pharmacol. Waldo 65 426 2004 10.1124/mol.65.2.426 Agonist binding and Gq-stimulating activities of the purified human P2Y1 receptor

상세보기
Purinergic Signal Erlinge 4 1 2008 10.1007/s11302-007-9078-7 P2 receptors in cardiovascular regulation and disease

상세보기
Biom. J. Suurvali 40 245 2017 P2X4: A fast and sensitive purinergic receptor
Pharmacol. Ther. von Kugelgen 110 415 2006 10.1016/j.pharmthera.2005.08.014 Pharmacological profile of cloned mammalian P2Y-receptor subtypes

상세보기
J. Biol. Chem. Joseph 278 23331 2003 10.1074/jbc.M302680200 Colocalization of ATP release sites and ecto-ATPase activity at the extracellular surface of human astrocytes

상세보기
Pharmacol. Rev. Ralevic 50 413 1998 Receptors for purines and pyrimidines

상세보기
J. Pharmacol. Exp. Ther. Kaiser 350 531 2014 10.1124/jpet.114.214569 A sulfonate, but not tanshinone II A, acts as potent negative allosteric modulator of the human purinergic receptor P2X7

상세보기
FEBS Lett. Locovei 580 239 2006 10.1016/j.febslet.2005.12.004 Activation of pannexin 1 channels by ATP through P2Y receptors and by cytoplasmic calcium

상세보기
Proc. Natl. Acad. Sci. U. S. A. Boycott 110 E3955 2013 10.1073/pnas.1309896110 Shear stress triggers insertion of voltage-gated potassium channels from intracellular compartments in atrial myocytes

상세보기
Cardiovasc. Res. Rucker-Martin 72 69 2006 10.1016/j.cardiores.2006.06.016 Chronic hemodynamic overload of the atria is an important factor for gap junction remodeling in human and rat hearts

상세보기
Exp. Cell Res. Goldberg 239 82 1998 10.1006/excr.1997.3872 Direct isolation and analysis of endogenous transjunctional ADP from Cx43 transfected C6 glioma cells

상세보기
Nat. Cell Biol. Goldberg 1 457 1999 10.1038/15693 Selective transfer of endogenous metabolites through gap junctions composed of different connexins

상세보기
Proc. Natl. Acad. Sci. U. S. A. Batra 109 3359 2012 10.1073/pnas.1115967109 Mechanical stress-activated integrin α5β1 induces opening of connexin 43 hemichannels

상세보기
Cardiovasc. Res. Meens 99 304 2013 10.1093/cvr/cvt095 Regulation of cardiovascular connexins by mechanical forces and junctions

상세보기
Biophys. J. Helmke 84 2691 2003 10.1016/S0006-3495(03)75074-7 Mapping mechanical strain of an endogenous cytoskeletal network in living endothelial cells

상세보기
Am. J. Phys. Bao 287 C1389 2004 10.1152/ajpcell.00220.2004 Connexins are mechanosensitive

상세보기
Proc. Natl. Acad. Sci. U. S. A. Contreras 100 11388 2003 10.1073/pnas.1434298100 Gating and regulation of connexin 43 (Cx43) hemichannels

상세보기
Eur. J. Cell Biol. Verma 88 79 2009 10.1016/j.ejcb.2008.08.005 Perturbing plasma membrane hemichannels attenuates calcium signalling in cardiac cells and HeLa cells expressing connexins

상세보기
FASEB J. Hu 15 2739 2001 10.1096/fj.01-0445fje A novel contractile phenotype with cardiac transgenic expression of the human P2X4 receptor

상세보기
Am. J. Physiol. Heart Circ. Physiol. Gregs 294 H1716 2008 10.1152/ajpheart.00945.2007 A positive inotropic effect of ATP in the human cardiac atrium

상세보기
Circul. Cardiovasc. Imaging Dodson 7 586 2014 10.1161/CIRCIMAGING.113.001472 Left atrial passive emptying function determined by cardiac magnetic resonance predicts atrial fibrillation recurrence after pulmonary vein isolation

상세보기
Am. J. Cardiol. Maron 113 1394 2014 10.1016/j.amjcard.2013.12.045 Left atrial remodeling in hypertrophic cardiomyopathy and susceptibility markers for atrial fibrillation identified by cardiovascular magnetic resonance

상세보기

LOADING...

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증