[논문]Non-invasive optical control of endogenous Ca 2+ channels in awake mice

Kim, Sungsoo; Kyung, Taeyoon; Chung, Jae-Hee; Kim, Nury; Keum, Sehoon; Lee, Jinsu; Park, Hyerim; Kim, Ho Min; Lee, Sangkyu; Shin, Hee-Sup; Heo, Won Do

doi:10.1038/s41467-019-14005-4

[해외논문] Non-invasive optical control of endogenous Ca 2+ channels in awake mice 원문보기

Nature communications, v.11 no.1 = v.11, 2020년, pp.210 -

Kim, Sungsoo (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea) , Kyung, Taeyoon (Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon, Republic of Korea) , Chung, Jae-Hee (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea) , Kim, Nury (Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon, Republic of Korea) , Keum, Sehoon (Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon, Republic of Korea) , Lee, Jinsu (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea) , Park, Hyerim (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea) , Kim, Ho Min (Center for Biomolecular and Cellular Structure, Institute for Basic Science (IBS), Daejeon, Republic of Korea) , Lee, Sangkyu (Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon, Republic of) , Shin, Hee-Sup , Heo, Won Do

Abstract ▼ AI-Helper

Optogenetic approaches for controlling Ca2+ channels provide powerful means for modulating diverse Ca2+-specific biological events in space and time. However, blue light-responsive photoreceptors are, in principle, considered inadequate for deep tissue stimulation unless accompanied by optic fiber insertion. Here, we present an ultra-light-sensitive optogenetic Ca2+ modulator, named monSTIM1 encompassing engineered cryptochrome2 for manipulating Ca2+ signaling in the brain of awake mice through non-invasive light delivery. Activation of monSTIM1 in either excitatory neurons or astrocytes of mice brain is able to induce Ca2+-dependent gene expression without any mechanical damage in the brain. Furthermore, we demonstrate that non-invasive Ca2+ modulation in neurons can be sufficiently and effectively translated into changes in behavioral phenotypes of awake mice.Optogenetic applications in the brain of live animals often require the use of optic fibers due to poor tissue-penetration of blue light. Here the authors present monSTIM1, an improved high sensitivity optogenetic tool able to modulate Ca2+ signaling in the brain of awake mice using non-invasive light stimulation.

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