[논문]Tissue mechanics drives regeneration of a mucociliated epidermis on the surface of Xenopus embryonic aggregates

Kim, Hye Young; Jackson, Timothy R.; Stuckenholz, Carsten; Davidson, Lance A.

doi:10.1038/s41467-020-14385-y

[해외논문] Tissue mechanics drives regeneration of a mucociliated epidermis on the surface of Xenopus embryonic aggregates 원문보기

Nature communications, v.11 no.1 = v.11, 2020년, pp.665 -

Kim, Hye Young (Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15213 USA) , Jackson, Timothy R. (Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15213 USA) , Stuckenholz, Carsten (Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15213 USA) , Davidson, Lance A. (Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15213 USA)

Abstract ▼ AI-Helper

Injury, surgery, and disease often disrupt tissues and it is the process of regeneration that aids the restoration of architecture and function. Regeneration can occur through multiple strategies including stem cell expansion, transdifferentiation, or proliferation of differentiated cells. We have identified a case of regeneration in Xenopus embryonic aggregates that restores a mucociliated epithelium from mesenchymal cells. Following disruption of embryonic tissue architecture and assembly of a compact mesenchymal aggregate, regeneration first restores an epithelium, transitioning from mesenchymal cells at the surface of the aggregate. Cells establish apico-basal polarity within 5 hours and a mucociliated epithelium within 24 hours. Regeneration coincides with nuclear translocation of the putative mechanotransducer YAP1 and a sharp increase in aggregate stiffness, and regeneration can be controlled by altering stiffness. We propose that regeneration of a mucociliated epithelium occurs in response to biophysical cues sensed by newly exposed cells on the surface of a disrupted mesenchymal tissue.The role of tissue mechanics in the regeneration of mucociliated epithelium in Xenopus is unclear. Here, the authors show that Xenopus ectoderm aggregates undergo epithelial-like phenotypic transition prior to differentiation of mucus-secreting goblet cells to enable regeneration.

참고문헌 (56)

1. Walentek P Quigley IK What we can learn from a tadpole about ciliopathies and airway diseases: using systems biology in Xenopus to study cilia and mucociliary epithelia Genesis 2017 55 e23001 10.1002/dvg.23001

상세보기
2. Dubaissi E Papalopulu N Embryonic frog epidermis: a model for the study of cell-cell interactions in the development of mucociliary disease Dis. Model Mech. 2011 4 179 192 10.1242/dmm.006494 21183475

상세보기
3. Quigley IK Stubbs JL Kintner C Specification of ion transport cells in the Xenopus larval skin Development 2011 138 705 714 10.1242/dev.055699 21266406

상세보기
4. Stubbs J Vladar E Axelrod J Kintner C Multicilin promotes centriole assembly and ciliogenesis during multiciliate cell differentiation Nat. Cell Biol. 2012 14 140 147 10.1038/ncb2406 22231168

상세보기
5. Walentek P A novel serotonin-secreting cell type regulates ciliary motility in the mucociliary epidermis of Xenopus tadpoles Development 2014 141 1526 1533 10.1242/dev.102343 24598162

상세보기
6. Dubaissi E A secretory cell type develops alongside multiciliated cells, ionocytes and goblet cells, and provides a protective, anti-infective function in the frog embryonic mucociliary epidermis Development 2014 141 1514 1525 10.1242/dev.102426 24598166

상세보기
7. Deblandre GA Wettstein DA Koyano-Nakagawa N Kintner C A two-step mechanism generates the spacing pattern of the ciliated cells in the skin of Xenopus embryos Development 1999 126 4715 4728 10518489
8. Stubbs JL Davidson L Keller R Kintner C Radial intercalation of ciliated cells during Xenopus skin development Development 2006 133 2507 2515 10.1242/dev.02417 16728476

상세보기
9. Maitre JL Asymmetric division of contractile domains couples cell positioning and fate specification Nature 2016 536 344 348 10.1038/nature18958 27487217

상세보기
10. Shyer AE Emergent cellular self-organization and mechanosensation initiate follicle pattern in the avian skin Science 2017 357 811 815 10.1126/science.aai7868 28705989

상세보기
11. Engler AJ Sen S Sweeney HL Discher DE Matrix elasticity directs stem cell lineage specification Cell 2006 126 677 689 10.1016/j.cell.2006.06.044 16923388

상세보기
12. McBeath R Pirone DM Nelson CM Bhadriraju K Chen CS Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment Developmental Cell 2004 6 483 495 10.1016/S1534-5807(04)00075-9 15068789

상세보기
13. Kilian KA Bugarija B Lahn BT Mrksich M Geometric cues for directing the differentiation of mesenchymal stem cells Proc. Natl Acad. Sci. USA 2010 107 4872 4877 10.1073/pnas.0903269107 20194780

상세보기
14. Merzdorf CS Chen YH Goodenough DA Formation of functional tight junctions in Xenopus embryos Developmental Biol. 1998 195 187 203 10.1006/dbio.1997.8846

상세보기
15. Jamrich M Sargent TD Dawid IB Cell-type-specific expression of epidermal cytokeratin genes during gastrulation of Xenopus laevis Genes Dev. 1987 1 124 132 10.1101/gad.1.2.124 2445625

상세보기
16. Chanet S Martin AC Mechanical force sensing in tissues Prog. Mol. Biol. Transl. Sci. 2014 126 317 10.1016/B978-0-12-394624-9.00013-0 25081624

상세보기
17. Dupont S Role of YAP/TAZ in mechanotransduction Nature 2011 474 179 183 10.1038/nature10137 21654799

상세보기
18. Aragona M A mechanical checkpoint controls multicellular growth through YAP/TAZ regulation by actin-processing factors Cell 2013 154 1047 1059 10.1016/j.cell.2013.07.042 23954413

상세보기
19. von Dassow M Strother JA Davidson LA Surprisingly simple mechanical behavior of a complex embryonic tissue PLoS ONE 2010 5 e15359 10.1371/journal.pone.0015359 21203396

상세보기
20. Turlier, H. & Maitre, J.-L. In Seminars in Cell & Developmental Biology , Vol. 47 110？117 (Elsevier, 2015).
21. Kurth T Immunocytochemical studies of the interactions of cadherins and catenins in the early Xenopus embryo Dev. Dyn. 1999 215 155 169 10.1002/(SICI)1097-0177(199906)215:2<155::AID-DVDY8>3.0.CO;2-S 10373020

상세보기
22. Jackson TR Kim HY Balakrishnan UL Stuckenholz C Davidson LA Spatiotemporally controlled mechanical cues drive progenitor mesenchymal-to-epithelial transition enabling proper heart formation and function Curr. Biol. 2017 27 1326 1335 10.1016/j.cub.2017.03.065 28434863

상세보기
23. Yam PT Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility J. Cell Biol. 2007 178 1207 1221 10.1083/jcb.200706012 17893245

상세보기
24. Kim HY Davidson LA Punctuated actin contractions during convergent extension and their permissive regulation by the non-canonical Wnt-signaling pathway J. Cell Sci. 2011 124 635 646 10.1242/jcs.067579 21266466

상세보기
25. Zhou J Kim HY Wang JH-C Davidson LA Macroscopic stiffening of embryonic tissues via microtubules, Rho-GEF, and assembly of contractile bundles of actomyosin Development 2010 137 2785 2794 10.1242/dev.045997 20630946

상세보기
26. Werner M Mitchell B Understanding ciliated epithelia: the power of Xenopus Genesis 2012 50 176 185 10.1002/dvg.20824 22083727

상세보기
27. Bragulla HH Homberger DG Structure and functions of keratin proteins in simple, stratified, keratinized and cornified epithelia J. Anat. 2009 214 516 559 10.1111/j.1469-7580.2009.01066.x 19422428

상세보기
28. Schroeder TE Neurulation in Xenopus laevis. An analysis and model based upon light and electron microscopy J. Embryol. Expt. Morphol. 1970 23 427 462
29. Whitsett JA Airway epithelial differentiation and mucociliary clearance Ann. Am. Thorac. Soc. 2018 15 S143 S148 10.1513/AnnalsATS.201802-128AW 30431340

상세보기
30. Kim HY Jackson TR Davidson LA On the role of mechanics in driving mesenchymal-to-epithelial transitions Semin Cell Dev. Biol. 2017 67 113 122 10.1016/j.semcdb.2016.05.011 27208723

상세보기
31. Rock JR Randell SH Hogan BL Airway basal stem cells: a perspective on their roles in epithelial homeostasis and remodeling Dis. Mod. Mech. 2010 3 545 556 10.1242/dmm.006031

상세보기
32. Gilbert PM Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture Science 2010 329 1078 1081 10.1126/science.1191035 20647425

상세보기
33. Fierro-Gonzalez JC White MD Silva JC Plachta N Cadherin-dependent filopodia control preimplantation embryo compaction Nat. Cell Biol. 2013 15 1424 1433 10.1038/ncb2875 24270889

상세보기
34. Maitre JL Niwayama R Turlier H Nedelec F Hiiragi T Pulsatile cell-autonomous contractility drives compaction in the mouse embryo Nat. Cell Biol. 2015 17 849 855 10.1038/ncb3185 26075357

상세보기
35. Korotkevich E The apical domain is required and sufficient for the first lineage segregation in the mouse embryo Dev. Cell 2017 40 235 247. e237 10.1016/j.devcel.2017.01.006 28171747

상세보기
36. Mongera A A fluid-to-solid jamming transition underlies vertebrate body axis elongation Nature 2018 561 1 10.1038/s41586-018-0479-2

상세보기
37. Kuroda H Fuentealba L Ikeda A Reversade B De Robertis E Default neural induction: neuralization of dissociated Xenopus cells is mediated by Ras/MAPK activation Genes Dev. 2005 19 1022 1027 10.1101/gad.1306605 15879552

상세보기
38. Ariizumi T Asashima M In vitro induction systems for analyses of amphibian organogenesis and body patterning Int J. Dev. Biol. 2001 45 273 279 11291857

상세보기
39. Sedzinski J Hannezo E Tu F Biro M Wallingford JB Emergence of an apical epithelial cell surface in vivo Dev. Cell 2016 36 24 35 10.1016/j.devcel.2015.12.013 26766441

상세보기
40. Stooke-Vaughan GA Davidson LA Woolner S Xenopus as a model for studies in mechanical stress and cell division Genesis 2017 55 e23004 10.1002/dvg.23004

상세보기
41. Stepien TL Lynch HE Yancey SX Dempsey L Davidson LA Using a continuum model to decipher the mechanics of embryonic tissue spreading from time-lapse image sequences: An approximate Bayesian computation approach PLoS ONE 2019 14 460774
42. Chien Y-H Keller R Kintner C Shook DR Mechanical strain determines the axis of planar polarity in ciliated epithelia Curr. Biol. 2015 25 2774 2784 10.1016/j.cub.2015.09.015 26441348

상세보기
43. Chien Y-H Srinivasan S Keller R Kintner C Mechanical strain determines cilia length, motility, and planar position in the left-right organizer Dev. Cell 2018 45 316 330. e314 10.1016/j.devcel.2018.04.007 29738711

상세보기
44. Sive, H. L., Grainger, R. M., Harland, R. M. (eds.) Early development of Xenopus laevis: a laboratory manual . (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2000).
45. Edlund AF Davidson LA Keller RE Cell segregation, mixing, and tissue pattern in the spinal cord of the Xenopus laevis neurula Dev. Dyn. 2013 242 1134 1146 10.1002/dvdy.24004 23813905

상세보기
46. Banko MR Chemical genetic screen for AMPKα2 substrates uncovers a network of proteins involved in mitosis Mol. Cell 2011 44 878 892 10.1016/j.molcel.2011.11.005 22137581

상세보기
47. Preibisch S Saalfeld S Tomancak P Globally optimal stitching of tiled 3D microscopic image acquisitions Bioinformatics 2009 25 1463 1465 10.1093/bioinformatics/btp184 19346324

상세보기
48. Zhou J Kim HY Davidson LA Actomyosin stiffens the vertebrate embryo during critical stages of elongation and neural tube closure Development 2009 136 677 688 10.1242/dev.026211 19168681

상세보기
49. Zhou J Pal S Maiti S Davidson LA Force production and mechanical adaptation during convergent extension Development 2015 142 692 701 10.1242/dev.116533 25670794

상세보기
50. Pfister K Shook DR Chang C Keller R Skoglund P Molecular model for force production and transmission during vertebrate gastrulation Development 2016 143 715 727 10.1242/dev.128090 26884399

상세보기
51. Rolo A Skoglund P Keller R Morphogenetic movements driving neural tube closure in Xenopus require myosin IIB Dev. Biol. 2009 327 327 338 10.1016/j.ydbio.2008.12.009 19121300

상세보기
52. Skoglund P Rolo A Chen X Gumbiner BM Keller R Convergence and extension at gastrulation require a myosin IIB-dependent cortical actin network Development 2008 135 2435 2444 10.1242/dev.014704 18550716

상세보기
53. von Dassow M Davidson LA Natural variation in embryo mechanics: gastrulation in Xenopus laevis is highly robust to variation in tissue stiffness Dev. Dyn. 2009 238 2 18 10.1002/dvdy.21809 19097119

상세보기
54. Schneider CA Rasband WS Eliceiri KW NIH Image to ImageJ: 25 years of image analysis Nat. Methods 2012 9 671 675 10.1038/nmeth.2089 22930834

상세보기
55. Sato M Levesque MJ Nerem RM An application of the micropipette technique to the measurement of the mechanical properties of cultured bovine aortic endothelial cells J. Biomech. Eng. 1987 109 27 34 10.1115/1.3138638 3560876

상세보기
56. ？indelka R Ferjentsik Z Jonak J Developmental expression profiles of Xenopus laevis reference genes Dev. Dyn. 2006 235 754 758 10.1002/dvdy.20665 16397894

상세보기

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