Sa, Young Hee
(Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine)
,
Lee, Ki Hwan
(Department of Chemistry, Kongju National University)
,
Hong, Seong-Karp
(Division of Bio and Health Sciences, Mokwon University)
베큘로바이러스 벡터가 uroplakin II promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) 등의 유전자로 재조합 되었다. 이렇게 재조합된 베큘로바이러스 벡터들은 여러가지 세포주에 감염을 시켰다. 우리는 이 재조합 벡터와 다른 대조 벡터를 비교하여 유전자 전달과 유전자 발현을 비교하였다. 그 결과 이 재조합 베큘로바이러스 벡터는 대조 벡터에 비하여 유전자 전달과 유전자 발현의 효율이 우수한 것으로 나타났다.
베큘로바이러스 벡터가 uroplakin II promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) 등의 유전자로 재조합 되었다. 이렇게 재조합된 베큘로바이러스 벡터들은 여러가지 세포주에 감염을 시켰다. 우리는 이 재조합 벡터와 다른 대조 벡터를 비교하여 유전자 전달과 유전자 발현을 비교하였다. 그 결과 이 재조합 베큘로바이러스 벡터는 대조 벡터에 비하여 유전자 전달과 유전자 발현의 효율이 우수한 것으로 나타났다.
Baculovirus vectors were recombined using uroplakin II promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) gene and so on. These novel recombinant vectors were infected into various cell lines. We performed ...
Baculovirus vectors were recombined using uroplakin II promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) gene and so on. These novel recombinant vectors were infected into various cell lines. We performed and analyzed gene transfer and gene expression of these recombinant vectors comparison with other control vectors. From this result, we identified that these recombinant vectors have higher efficient gene transfer and expression of than control vector.
Baculovirus vectors were recombined using uroplakin II promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) gene and so on. These novel recombinant vectors were infected into various cell lines. We performed and analyzed gene transfer and gene expression of these recombinant vectors comparison with other control vectors. From this result, we identified that these recombinant vectors have higher efficient gene transfer and expression of than control vector.
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제안 방법
Baculovirus vector was reconstructed in this study. These vectors were included genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, uroplakin II promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD).
이론/모형
1-Tat were subcloned into the NdeI/BamHI sites of pET-15b Clontech, USA), generating pEGFP-Tat. The ABI Prism automated sequencing method was used to confirm the nucleotide sequences of all the PCR products.
성능/효과
Transduction efficiency of reconstructed baculovirus vectors (pBac-EGFP and pBacG-EGFP-PTD) were compared in infected cell lines (NIH293, 293T, SNU46, Hur7, HepG2, and HFF) (Fig. 2).
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