IPC분류정보
국가/구분 |
United States(US) Patent
공개
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0400290
(2003-03-25)
|
공개번호 |
US-0092002
(2004-05-13)
|
우선권정보 |
KR-0070114 (2002-11-12) |
발명자
/ 주소 |
- Kim, Byung-Kyu
- Park, Jung-Yul
- Kim, Deok-Ho
- Park, Jong-Oh
|
대리인 / 주소 |
ARMSTRONG, WESTERMAN & HATTORI, LLP
|
인용정보 |
피인용 횟수 :
0 인용 특허 :
0 |
초록
▼
This new automatic bio-manipulation factory system for manipulating a single cell comprises: a bio-MEMS that transports, separates, and extracts a single cell for manipulation from a plurality of cells, controls the rotation-orientation of the extracted cell automatically, and enables the extracted
This new automatic bio-manipulation factory system for manipulating a single cell comprises: a bio-MEMS that transports, separates, and extracts a single cell for manipulation from a plurality of cells, controls the rotation-orientation of the extracted cell automatically, and enables the extracted cell to maintain a certain temperature; and a micro manipulation system that obtains vision information and manipulation information about the extracted single cell and the manipulation tool, provides such information to operator, manipulates the extracted single cell by transmitting the rotation-orientation feedback information and the cell manipulation order of the operator to the bio-MEMS, and manipulates the manipulation tool by transmitting the tool manipulation order of the operator to the manipulation tool. The conventional manual and individualized single cell manipulation can be automated and processed collectively by systematically combining the bio-MEMS with the micro manipulation system.
대표청구항
▼
1. An automatic bio-manipulation factory system for manipulating a single cell comprising:a bio-MEMS (Micro-Electro Mechanical System) that transports, separates, and extracts a single cell for manipulation from a plurality of cells, controls the rotation-orientation of the extracted cell automatica
1. An automatic bio-manipulation factory system for manipulating a single cell comprising:a bio-MEMS (Micro-Electro Mechanical System) that transports, separates, and extracts a single cell for manipulation from a plurality of cells, controls the rotation-orientation of the extracted cell automatically, and enables the extracted cell to maintain a constant temperature; and a micro manipulation system that obtains vision information and manipulation information about the extracted single cell and the manipulation tool, provides such information to operator, manipulates the extracted single cell by transmitting the rotation-orientation feedback information and the cell manipulation order of the operator to the bio-MEMS, and manipulates the manipulation tool by transmitting the tool manipulation order of the operator to the manipulation tool. 2. The automatic bio-manipulation factory system for manipulating a single cell in claim 1, wherein the bio-MEMS comprises:an input unit that stores a plurality of inputted cells and transports the plurality of cells in a line by controlling the size of the cell transportation channel through the use of the capillary electrophoresis caused by application of the transportation voltage and the focus voltage; an injection unit including an injection port that separates cells for manipulation from the plurality of cells transported in a line by applying the separation voltage; an extraction unit that extracts one of the cells separated for manipulation by applying the extraction voltage and manipulates the rotation-orientation of the cell by applying the rotation-orientation voltage; and a drain unit that absorbs the rest of the cells other than the cells separated for manipulation. 3. The automatic bio-manipulation factory system for manipulating a single cell of claim 2, wherein the input unit comprises:a first focus port that transports the first flow by applying the first focus voltage; a second focus port that transports the second flow by applying the second focus voltage which is the same as the first focus voltage; and an input cell storage port that stores a plurality of the inputted cells and transports the plurality of cells though the third flow to the cell transportation channel formed between the first flow and the second flow by applying the transportation voltage. 4. The automatic bio-manipulation factory system for manipulating a single cell of claim 3, wherein the size of the cell transportation channel is such that the plurality of cells may pass in one line. 5. The automatic bio-manipulation factory system for manipulating a single cell of claim 2, the extraction unit comprises:a conductive polymer that extracts only one out of the cells separated for manipulation; an extraction controller that controls the cell extraction of the conductive polymer; and a cell manipulator that controls temperature of the extracted single cell and rotation-orientation of the extracted single cell by applying the rotation-orientation voltage. 6. The automatic bio-manipulation factory system for manipulating a single cell of claim 5, wherein the conductive polymer is made of poly pyrrole. 7. The automatic bio-manipulation factory system for manipulating a single cell of claim 5, wherein the cell manipulator comprises:an extraction port that absorbs the single cell extracted for manipulation by applying the extraction voltage; a heater, included in the extraction port, that manipulates temperature of the extracted single cell; a temperature controller that controls the heater's manipulation of the cell temperature; rotation-orientation electrodes, located around the extraction port, that manipulate the rotation-orientation of the extracted single cell using the dielectrophoresis by applying the rotation-orientation voltage; and a rotation-orientation controller that receives rotation-orientation feedback information and cell manipulation order of the operator and then controls the rotation-orientation electrodes' manipulation of the cell rotation-orientation. 8. The automatic bio-manipulation factory system for manipulating a single cell of claim 7, wherein the rotation-orientation controller controls automatically the rotation-orientation of the extracted single cell so that it may be located at the operator's desired rotation-orientation or at the previously inputted rotation-orientation according to the rotation-orientation feedback information. 9. The automatic bio-manipulation factory system for manipulating a single cell of claim 2, wherein the drain unit comprises:a third focus port that absorbs the first flow by applying the third focus voltage; a fourth focus port that absorbs the second flow by applying the fourth focus voltage which is the same as the third focus voltage; and a drain cell storage port that stores the rest of the cells other than the cell separated for manipulation through the third flow from the cell transportation channel formed between the first flow and the second flow, by applying the drain voltage. 10. The automatic bio-manipulation factory system for manipulating a single cell of claim 1, wherein the micro manipulation system comprises:a vision unit that monitors the extracted single cell and the manipulation tool by using a microscope in real-time and then outputs vision information and rotation-orientation feedback information; a haptic unit, equipped with a force sensor measuring changes in th e cell manipulation force, that collects and outputs information on the extracted single cell and the manipulation tool and that receives tool manipulation order of the operator and then outputs it to the manipulation tool; a control unit that provides the operator with the outputted vision information and manipulation information, outputs the cell manipulation order of the operator to an extraction unit, and outputs the tool manipulation order of the operator to the haptic unit; a biomimic smart micro pipette that senses the cell manipulation force, outputs trigger information, and manipulates the cell according to the tool manipulation order of the operator; and a holding pipette that controls the rotation-orientation of a cell at the time of manipulating the cell and then fixes the orientation of the cell according to the tool manipulation order of the operator which has been transmitted from the control unit. 11. The automatic bio-manipulation factory system for manipulating a single cell of claim 10, wherein the microscope, which has the fluorescence function, is a confocal microscope or an optical microscope. 12. The automatic bio-manipulation factory system for manipulating a single cell of claim 10, wherein the vision unit outputs the rotation-orientation feedback information based on the vision information acquired by the microscope's monitoring and controls automatically the rotation-orientation of the extracted single cell so that it may be located at the operator's desired rotation-orientation or at the previously inputted rotation-orientation. 13. The automatic bio-manipulation factory system for manipulating a single cell of claim 10, wherein the biomimic smart micro pipette comprises:a physical force sensor that senses manipulation force at the time of manipulating the cell; a trigger sensor that outputs trigger information at the time of contacting the cell; a pointed tip that vents chemical material which may melt zonapellucida of the cell when a trigger signal is generated by the contact of the cell; an injection mechanism part that enables accurate manipulation while imposing the minimum invasion on the cell at the time of manipulating the cell by using the rotation and impact drive mechanism; and an injector that enables the injection of chemical material or bio-material necessary for the cell manipulation. 14. The automatic bio-manipulation factory system for manipulating a single cell of claim 13, wherein the trigger sensor is either an electric trigger sensor that outputs the trigger signal by the change in the potential difference of the cell surface or a bio trigger sensor that accepts the galactosyltransferase, which is bio trigger material of a spermatozoon.
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