[미국특허]
METHODS, PANELS OF IDENTIFICATION MARKERS, AND KITS FOR IDENTIFYING FORENSIC SAMPLES
원문보기
IPC분류정보
국가/구분
United States(US) Patent
공개
국제특허분류(IPC7판)
C40B-030/00
C40B-040/06
출원번호
US-0242911
(2011-09-23)
공개번호
US-0015832
(2012-01-19)
발명자
/ 주소
FANG, Rixun N.
Burns, John W.
Furtado, Manohar R.
출원인 / 주소
APPLIED BIOSYSTEMS, LLC
인용정보
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초록▼
Methods for identifying forensic samples using panels of markers and gene expression profiling, including without limitation, mRNA profiling, miRNA profiling, or both, are disclosed. Panels of markers for identifying certain tissue samples and certain body fluid samples are also disclosed. Kits for
Methods for identifying forensic samples using panels of markers and gene expression profiling, including without limitation, mRNA profiling, miRNA profiling, or both, are disclosed. Panels of markers for identifying certain tissue samples and certain body fluid samples are also disclosed. Kits for expediting performance of certain of the disclosed methods are provided.
대표청구항▼
1-6. (canceled) 7. A set of primers specific for a panel of markers comprising, at least one brain marker comprising at least one of miR-125b, miR-128a, miR-128b, miR-129, miR-135, and miR-153; at least one muscle marker comprising at least one of miR-1d, miR-133a, miR-133b, miR-296, miR-208; at lea
1-6. (canceled) 7. A set of primers specific for a panel of markers comprising, at least one brain marker comprising at least one of miR-125b, miR-128a, miR-128b, miR-129, miR-135, and miR-153; at least one muscle marker comprising at least one of miR-1d, miR-133a, miR-133b, miR-296, miR-208; at least one kidney marker comprising at least one of miR-192, miR-204, miR-215, and miR-216; at least one thymus marker comprising at least one of miR-96 and miR-182; at least one testes marker comprising at least one of miR-10b and let-7e; and at least one placenta marker comprising at least one of miR-141 and miR-23a. 8. A set of primers specific for a panel of markers comprising at least one mRNA marker and at least one miRNA marker. 9. (canceled) 10. (canceled) 11. A method for identifying a forensic sample containing nucleic acid comprising: combining a set of primer pairs to specifically amplify each gene in a panel of markers, wherein the panel of markers comprise at least one brain marker comprising at least one of miR-125b, miR-128a, miR-128b, miR-129, miR-135, and miR-153; at least one muscle marker comprising at least one of miR-1d, miR-133a, miR-133b, miR-296, miR-208; at least one kidney marker comprising at least one of miR-192, miR-204, miR-215, and miR-216; at least one thymus marker comprising at least one of miR-96 and miR-182; at least one testes marker comprising at least one of miR-10b and let-7e; and at least one placenta marker comprising at least one of miR-141 and miR-23a with at least some of the nucleic acid from the sample and a polymerase, wherein the at least one first primer in the set of marker-specific primers is designed to amplify at least a portion of a first member of a panel of markers and at least one second primer in the set of marker-specific primers is designed to amplify at least a portion of a second member of the panel of markers;generating an expression profile for the panel of markers; andidentifying the forensic sample. 12. The method of claim 11, wherein at least one marker-specific primer comprises a marker-specific primer pair. 13. (canceled) 14. The method of claim 11, wherein the generating comprises a reporter probe, a substrate, a nucleic acid dye, a microfluidics device, or combinations thereof. 15. The method of claim 11, wherein the polymerase comprises a DNA-dependent DNA polymerase or an RNA-dependent DNA polymerase and a DNA-dependent DNA polymerase. 16. The method of claim 11, wherein the generating comprises a first amplification reaction comprising a multiplicity of different gene-specific primer pairs and a second amplification reaction comprising a single-plex amplification reaction. 17. The method of claim 11, wherein the second amplification reaction comprises a multiplicity of different single-plex amplification reactions performed in parallel. 18. The method of claim 17, wherein the multiplicity of parallel single-plex amplification reactions comprise a microfluidics device. 19. The method of claim 11, wherein at least one gene-specific primer comprises a miRNA-specific primer. 20. The method of claim 19, wherein the at least one miRNA-specific primer comprises at least one linker probe; and further comprising a reporter probe. 21. The method of claim 20, wherein the generating comprises a first amplification reaction and a second amplification reaction. 22. A kit comprising of a set of primers specific for a panel of markers, wherein the set of primers consists of primer pairs to specifically amplify each of the markers in the panel of markers, wherein the panel of markers comprise at least one brain marker comprising at least one of miR-125b, miR-128a, miR-128b, miR-129, miR-135, and miR-153; at least one muscle marker comprising at least one of miR-1d, miR-133a, miR-133b, miR-296, miR-208; at least one kidney marker comprising at least one of miR-192, miR-204, miR-215, and miR-216; at least one thymus marker comprising at least one of miR-96 and miR-182; at least one testes marker comprising at least one of miR-10b and let-7e; and at least one placenta marker comprising at least one of miR-141 and miR-23a, wherein the set of primers consists of 21 or fewer primer pairs. 23. The kit of claim 22, further comprising one or more of a polymerase; nucleotide triphosphates (NTPs), wherein the NTPs can be ribonucleotide triphosphates (rNTPs) and/or deoxyribonucleotide triphosphates (dNTPs); a reporter probe; a nucleic acid dye; a miRNA linker probe; an internal reference dye; a control sequence or internal standard; a reporter group, wherein the reporter group includes an NTP comprising a reporter group; orcombinations thereof. 24. The kit of claim 22, further including a microarray and/or a microfluidics device. 25. The kit of claim 22, wherein the polymerase comprises a DNA-dependent DNA polymerase or an RNA-dependent DNA polymerase and a DNA-dependent DNA polymerase.
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