CELL IDENTIFYING APPARATUS AND CELL IDENTIFYING METHOD
원문보기
IPC분류정보
국가/구분
United States(US) Patent
공개
국제특허분류(IPC7판)
G01N-021/64
C12M-001/34
출원번호
US-0342245
(2012-01-03)
공개번호
US-0171700
(2012-07-05)
발명자
/ 주소
Takahashi, Toru
Tsukii, Ken
Xu, Jie
출원인 / 주소
FURUKAWA ELECTRIC CO., LTD.
인용정보
피인용 횟수 :
0인용 특허 :
0
초록▼
A cell identifying apparatus includes a capillary through which a sample flow passes, an irradiating unit that irradiates irradiation light, a light receiving unit that receives transmitted light and outputs a transmitted light signal based on the transmitted light, a measuring unit that receives th
A cell identifying apparatus includes a capillary through which a sample flow passes, an irradiating unit that irradiates irradiation light, a light receiving unit that receives transmitted light and outputs a transmitted light signal based on the transmitted light, a measuring unit that receives the transmitted light signal output from the light receiving unit and measures the change of the transmitted light signal caused by cells, an identifying unit that identifies whether the cells are peripheral blood cells on the basis of a value of an extreme point in the change of the transmitted light signal, the change being caused by the cells and measured by the measuring unit, a sorting unit that sorts the cells in a predetermined vessel, on the basis of the identification result of the identifying unit, and a supplying unit that supplies the sample flow to the capillary.
대표청구항▼
1. A cell identifying apparatus that identifies different cells or cell species in peripheral blood, comprising: an irradiating unit that irradiates irradiation light onto a liquid in which cells in the peripheral blood are dispersed;a light receiving unit that is provided at a position directly fac
1. A cell identifying apparatus that identifies different cells or cell species in peripheral blood, comprising: an irradiating unit that irradiates irradiation light onto a liquid in which cells in the peripheral blood are dispersed;a light receiving unit that is provided at a position directly facing the irradiating unit, receives transmitted light through the liquid based on the irradiation light irradiated from the irradiating unit onto the liquid in a state in which relative positions of the cells with respect to the irradiation light change at a constant speed, and outputs a transmitted light signal based on the transmitted light;a measuring unit that receives the transmitted light signal output from the light receiving unit and measures a change of the transmitted light signal, the change being caused by the cells; andan identifying unit that identifies the cells in the peripheral blood, on the basis of a value of an extreme point in the change of the transmitted light signal, the change being caused by the cells and measured by the measuring unit. 2. The cell identifying apparatus of claim 1, wherein, when a value of the transmitted light signal obtained based on the liquid having no cells is set as a reference value, the identifying unit compares a value of a maximum point in the change of the transmitted light signal caused by the cells and the reference value, and identifies the cells as cells other than peripheral blood cells when the value of the maximum point is greater than or equal to the reference value. 3. The cell identifying apparatus of claim 1 or 2, wherein the cells that are identified as the cells other than the peripheral blood cells by the identifying unit are circulating cancer cells. 4. The cell identifying apparatus of any one of claims 1 to 3, wherein the cells are cells on which fluorescent processing including an antibody/antigen reaction of a cell surface and expression of fluorescent proteins in the cells is performed. 5. The cell identifying apparatus of any one of claims 1 to 4, wherein the measuring unit measures transmitted light information based on the change of the transmitted light signal, andthe identifying unit identifies the cells in the peripheral blood, on the basis of the transmitted light information measured by the measuring unit. 6. The cell identifying apparatus of any one of claims 1 to 5, further comprising: a second light receiving unit that is provided at a position other than the position directly facing the irradiating unit, receives scattered light and/or fluorescent light of the cells based on the irradiation light irradiated by the irradiating unit, and outputs a scattered/fluorescent signal based on the scattered light and/or fluorescent light,wherein the measuring unit receives the scattered/fluorescent signal output from the second light receiving unit and measures the change of the scattered/fluorescent signal caused by the cells, andthe identifying unit identifies the cells in the peripheral blood, on the basis of the change of the scattered/:fluorescent signal measured by the measuring unit. 7. The cell identifying apparatus of any one of claims 1 to 6, wherein the measuring unit measures scattered/fluorescent information based on the change of the scattered/fluorescent signal, andthe identifying unit identifies the cells in the peripheral blood on the basis of the scattered/fluorescent information measured by the measuring unit. 8. The cell identifying apparatus of any one of claims 1 to 7, wherein the irradiation light is non-condensed single-mode light. 9. The cell identifying apparatus of any one of claims 1 to 8, further comprising: a sorting unit that sorts the cells in the peripheral blood identified by the identifying unit in a predetermined vessel. 10. A cell identifying method that identifies cells in peripheral blood using an irradiating unit configured to irradiate irradiation light onto a liquid in which the cells in the peripheral blood are dispersed, and a light receiving unit that is provided at a position directly facing the irradiating unit, receives transmitted light, and outputs a transmitted light signal based on the transmitted light to a measuring unit, the cell identifying method comprising: (a) a step of irradiating irradiation light from the irradiating unit onto the liquid in a state in which relative positions of the cells with respect to the irradiation light change at a constant speed;(b) a step of receiving transmitted light through the liquid based on the irradiation light by the light receiving unit and outputting a transmitted light signal based on the transmitted light to the measuring unit;(c) a step of receiving the transmitted light signal output in the step (b) by the measuring unit and measuring the change of the transmitted light signal caused by the cells with the measuring unit; and(d) a step of identifying the cells in the peripheral blood, on the basis of a value of an extreme point in the change of the transmitted light signal caused by the cells, which is measured in the step (c). 11. The cell identifying method of claim 10, wherein, in the step (d), when a value of the transmitted light signal obtained based on the liquid having no cells is set as a reference value, a value of a maximum point in the change of the transmitted light signal caused by the cells and the reference value are compared, and the cells are identified as cells other than peripheral blood cells when the value of the maximum point is greater than or equal to the reference value. 12. The cell identifying method of claim 10 or 11, wherein the cells that are identified as the cells other than the peripheral blood cells in the step (d) are circulating cancer cells. 13. The cell identifying method of any one of claims 10 to 12, wherein the cells are cells on which fluorescent processing including an antibody/antigen reaction of a cell surface and expression of fluorescent proteins in the cells is performed. 14. The cell identifying method of any one of claims 10 to 13, wherein, in the step (c), transmitted light information is measured based on the change of the transmitted light signal, andin the step (d), the cells in the peripheral blood are identified on the basis of the transmitted light information measured in the step (c). 15. The cell identifying method of any one of claims 10 to 14, wherein a second light receiving unit is further used, and the second light receiving unit is provided at a position other than the position directly facing the irradiating unit, receives scattered light and/or fluorescent light of the cells based on the irradiation light irradiated by the irradiating unit, and outputs a scattered/fluorescent signal based on the scattered light and/or fluorescent light to the measuring unit,in the step (b), the scattered light and/or fluorescent light of the cells based on the irradiation light are/is received by the second light receiving unit and a scattered/fluorescent signal based on the scattered light and/or fluorescent light is output to the measuring unit,in the step (c), the scattered/fluorescent signal output in the step (b) is received by the measuring unit and the change of the scattered/fluorescent signal caused by the cells is measured by the measuring unit, andin the step (d), the cells in the peripheral blood are identified on the basis of the change of the scattered/fluorescent signal measured in the step (c). 16. The cell identifying method of any one of claims 10 to 15, wherein, in the step (c), scattered/fluorescent information is measured based on the change of the scattered/fluorescent signal, andin the step (d), the cells in the peripheral blood are identified on the basis of the scattered/fluorescent information measured in the step (c). 17. The cell identifying method of any one of claims 10 to 16, wherein the irradiation light is non-condensed single-mode light. 18. The cell identifying method of any one of claims 10 to 17, further comprising: (e) a step of sorting the cells in the peripheral blood identified in the step (d) in a predetermined vessel.
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