초록 ▼

This invention relates generally to the detection of genetic differences among soybeans. More particularly, the invention relates to soybean quantitative trait loci (QTL) for tolerance to protoporphyrinogen oxidase inhibitors, to soybean plants possessing these QTLs, which map to a novel chromosomal

This invention relates generally to the detection of genetic differences among soybeans. More particularly, the invention relates to soybean quantitative trait loci (QTL) for tolerance to protoporphyrinogen oxidase inhibitors, to soybean plants possessing these QTLs, which map to a novel chromosomal region, and to genetic markers that are indicative of phenotypes associated with protoporphyrinogen oxidase inhibitor tolerance. Methods and compositions for use of these markers in genotyping of soybean and selection are also disclosed.

대표청구항 ▼

1. A method of selecting a soybean plant or germplasm with tolerance or improved tolerance to herbicides that inhibit protoporphyrinogen oxidase function, the method comprising: a) detecting in a soybean plant or germplasm at least one allele of a marker locus that is associated with the tolerance o

1. A method of selecting a soybean plant or germplasm with tolerance or improved tolerance to herbicides that inhibit protoporphyrinogen oxidase function, the method comprising: a) detecting in a soybean plant or germplasm at least one allele of a marker locus that is associated with the tolerance or improved tolerance to herbicides that inhibit protoporphyrinogen oxidase function, wherein the one or more marker locus is a marker locus localizing within a chromosome interval flanked by and including S01659-1-A and S03859-1-A on linkage group L; andb) selecting the soybean plant or germplasm comprising the at least one allele of one or more marker locus, thereby selecting a soybean plant with tolerance or improved tolerance to herbicides that inhibit protoporphyrinogen oxidase function. 2. The method of claim 1, wherein the marker locus is selected from the group consisting of S08102-1-Q1, S08103-1-Q1. S08104-1-Q1, S08106-1-Q1, S08107-1-Q1, S08107-1-Q1, S08109-1-Q1, S08110-1-Q1, S08111-1-Q1, S08115-2-Q1, S08117-1-Q1, S08119-1-Q1, S08116-1-Q1, S08112-1-Q1, S08108-1-Q1, S08101-4-Q1, S08101-1-Q1, S08101-2-Q1, and S08101-3-Q1. 3. The method of claim 1, wherein said quantitative trait locus is localized to a chromosomal interval defined by and including S08119-1-Q1 and S08101-3-Q1 on linkage group L. 4. The method of claim 1, wherein said quantitative trait locus is localized to a chromosomal interval defined by and including markers S08116-1-Q1 and S08101-2-Q1 on linkage group L. 5. The method of claim 1, wherein said quantitative trait locus is localized to a chromosomal interval defined by and including markers S08101-1-Q1 and S08101-1-Q1 on linkage group L. 6. The method of claim 1, wherein said quantitative trait locus is localized to a chromosomal interval defined by and including markers S08112-1-Q1 and S08108-1-Q1 on linkage group L. 7. The method of claim 1, wherein the herbicide is selected from the group consisting of diphenylethers, N-phenylpthalamides, oxadiazole and triazolinones. 8. The method of claim 7, wherein the herbicide is selected from the group consisting of sulfentrazone, carfentrazone-ethyl, aciflourfen, lactofen, fomesafen, flumioxazin, flumiclorac-pentyl and oxyfluorfen. 9. The method of claim 1, wherein the selection occurs as part of further breeding to improve a soybean variety's tolerance to one or more herbicides that inhibit protoporphyrinogen oxidase function. 10. The method of claim 9, wherein the further breeding is selected from the group consisting of additional crosses with other lines, hybrids, backcrossing, self-crossing, and combinations thereof. 11. A soybean plant selected by the method of claim 1. 12. A kit for selecting at least one soybean plant by marker assisted selection of a quantitative trait locus associated with tolerance to herbicides that inhibit protoporphyrinogen oxidase function comprising: a. primers for detecting at least one tolerance-associated marker locus, wherein the tolerance-associated locus is selected from the group consisting of S08102-1-Q1, S08103-1-Q1. S08104-1-Q1, S08106-1-Q1, S08107-1-Q1, S08107-1-Q1, S08109-1-Q1, S08110-1-Q1, S08111-1-Q1, S08115-2-Q1, S08117-1-Q1, S08119-1-Q1, S08116-1-Q1, S08112-1-Q1, S08108-1-Q1, S08101-4-Q1, S08101-1-Q1, S08101-2-Q1, and S08101-3-Q1; andb. instructions in using the primers or probes for detecting the marker loci and correlating the loci with predicted protoporphyrinogen oxidase inhibitor tolerance. 13. A method for selectively controlling weeds in a field containing a soybean crop comprising: (a) planting a field with crop seeds or plants which are tolerant to herbicides that inhibit protoporphyrinogen oxidase function as a result of comprising a tolerance allele in a marker localizing within a chromosomal interval flanked by and including S01659-1-A and S03859-1-A on linkage group L; and(b) applying a sufficient amount of a herbicide that inhibits protoporphyrinogen oxidase function to control the weeds without significantly affecting the crop. 14. The method of claim 13, wherein the marker is selected from the group consisting of S08102-1-Q1, S08103-1-Q1. S08104-1-Q1, S08106-1-Q1, S08107-1-Q1, S08107-1-Q1, S08109-1-Q1, S08110-1-Q1, S08111-1-Q1, S08115-2-Q1, S08117-1-Q1, S08119-1-Q1, S08116-1-Q1, S08112-1-Q1, S08108-1-Q1, S08101-4-Q1, S08101-1-Q1, S08101-2-Q1, and S08101-3-Q1. 15. The method of claim 13, wherein said marker is localized to a chromosomal interval defined by and including S08119-1-Q1 and S08101-3-Q1 on linkage group L. 16. The method of claim 13, wherein said marker is localized to a chromosomal interval defined by and including markers S08116-1-Q1 and S08101-2-Q1 on linkage group L. 17. The method of claim 13, wherein said marker is localized to a chromosomal interval defined by and including markers S08101-1-Q1 and S08101-1-Q1 on linkage group L. 18. The method of claim 13, wherein said marker is localized to a chromosomal interval defined by and including markers S08112-1-Q1 and S08108-1-Q1 on linkage group L. 19. The method of claim 13, wherein the herbicide is applied as a pre-emergent herbicide. 20. The method of claim 13, wherein the herbicide is applied as a post-emergent herbicide. 21. The method of claim 13, further comprising applying to the crop and weeds in the field a simultaneous or chronologically staggered application of a herbicide that inhibits protoporphyrinogen oxidase function and optionally an additional herbicide formulation. 22. The method of claim 21, wherein the additional herbicide formulation is applied and the herbicide formulation contains an active ingredient selected from the group consisting of a hydroxyphenylpyruvatedioxygenase inhibitor, a glyphosate, a sulfonylurea, a sulfonamide, an imidazolinone, a bialaphos, a phosphinothricin, an azafenidin, a butafenacil, a sulfosate, a glufosinate, a dicamba, and a protox inhibitor. 23. The method of claim 22, wherein said additional herbicide formulation is applied simultaneously or sequentially. 24. The method of claim 22, wherein said crop seeds or plants further comprise tolerance to the active ingredient of the additional herbicide formulation. 25. The method of claim 24, wherein tolerance to the active ingredient of the additional herbicide formulation is provided by insertion of a transgene which confers the tolerance. 26. A method for selectively screening soybean plants for herbicide tolerance comprising: (a) planting soybean seeds or plants comprising a marker localizing within a chromosomal interval flanked by and including S01659-1-A and S03859-1-A on linkage group L; and(b) treating the plants by applying a sufficient amount of a herbicide that inhibits protoporphyrinogen oxidase function to differentiate between susceptible and tolerant plants;(c) scoring the treated plants for tolerance to the herbicide. 27. The method of claim 26, wherein the marker is selected from the group consisting of S08102-1-Q1, S08103-1-Q1. S08104-1-Q1, S08106-1-Q1, S08107-1-Q1, S08107-1-Q1, S08109-1-Q1, S08110-1-Q1, S08111-1-Q1, S08115-2-Q1, S08117-1-Q1, S08119-1-Q1, S08116-1-Q1, S08112-1-Q1, S08108-1-Q1, S08101-4-Q1, S08101-1-Q1, S08101-2-Q1, and S08101-3-Q1. 28. The method of claim 26, wherein said marker is localized to a chromosomal interval defined by and including S08119-1-Q1 and S08101-3-Q1 on linkage group L. 29. The method of claim 26, wherein said marker is localized to a chromosomal interval defined by and including markers S08116-1-Q1 and S08101-2-Q1 on linkage group L. 30. The method of claim 26, wherein said marker is localized to a chromosomal interval defined by and including markers S08101-1-Q1 and S08101-1-Q1 on linkage group L. 31. The method of claim 26, wherein said marker is localized to a chromosomal interval defined by and including markers S08112-1-Q1 and S08108-1-Q1 on linkage group L. 32. A plant selected by the method of claim 1.