The invention relates to novel self-assembling peptide complexes comprising a charged peptide and a polysaccharide, methods of producing them and uses therefor. The novel self-assembling peptide complexes of the present invention have particular utility in the restoration of biomechanical or biochem
The invention relates to novel self-assembling peptide complexes comprising a charged peptide and a polysaccharide, methods of producing them and uses therefor. The novel self-assembling peptide complexes of the present invention have particular utility in the restoration of biomechanical or biochemical function of a variety of biological tissues, for example and without limitation, in degenerated spinal discs, osteoarthritic joints, damaged cartilage, meniscus, ligaments, tendons, dental, ophthalmic and cardiovascular and blood vessel tissues. The invention provides inter alia methods of repairing and or restoring biomechanical or biochemical function of biological tissues and scaffolds for the support of cell growth.
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1. A composition of matter comprising a self-assembling peptide complex, the complex comprising: (i) at least one charged peptide capable of self-assembling, and;(ii) an oppositely or same charged polysaccharide. 2. The composition according to claim 1, wherein the peptide capable of self-assembling
1. A composition of matter comprising a self-assembling peptide complex, the complex comprising: (i) at least one charged peptide capable of self-assembling, and;(ii) an oppositely or same charged polysaccharide. 2. The composition according to claim 1, wherein the peptide capable of self-assembling is positively charged and is selected from the group consisting of P11-12 (SEQ ID NO:2), P11-8 (SEQ ID NO:1), P11-14 (SEQ ID NO: 3), P11-16 (SEQ ID NO: 4), P11-18 (SEQ ID NO:5), P11-19 (SEQ ID NO:6), P11-26 (SEQ ID NO:7), P11-28 (SEQ ID NO:8), P11-31 (SEQ ID NO:9) and P11-32 (SEQ ID NO:10). 3. The composition according to claim 1, wherein the overall net positive charge on the peptide is +2, +4 or +6. 4. (canceled) 5. The composition of claim 1, wherein; the amino acid residues at positions 5 and 7 of the peptide is ornithine (O);the amino acid residues at positions 1 and 2 on the peptide are the same or different but reversed at positions 10 and 11;the amino acid residue at position 3 on the peptide is either ornithine (O) or arginine (R);the amino acid residue at positions 4 and 8 of the peptide is either phenylalanine (F) or glutamine (Q);the amino acid residue at position 6 of the peptide is either tryptophan (W) or glutamine (Q); orthe amino acid residue at position 9 of the peptide is either glutamic acid (E) or glutamine (Q). 6.-10. (canceled) 11. The composition according to claim 1, wherein the charged polysaccharide is natural or synthetic. 12. The composition according to claim 1, wherein the peptide capable of self-assembling is positively charged peptide and is associated with a polysaccharide selected from the group consisting of glycosaminoglycan (GAG), oligosaccharide, mucopolysaccharide and dextran. 13. The composition according to claim 12, wherein the polysaccharide is negatively charged and is a glycosaminoglycan (GAG) selected from the group consisting of chondroitin sulfate, dermatan sulfate, kertatan sulfate, hyaluronan, hyaluronic acid, heparin and heparan sulphate. 14. A composition according to claim 1, wherein the self-assembling peptide complex is P11-12 (SEQ ID NO: 2): GAG, P11-8 (SEQ ID NO: 1): GAG, P11-14 (SEQ ID NO: 3): GAG, P11-16 (SEQ ID NO: 4): GAG, P11-18 (SEQ ID NO: 5): GAG, P11-19 (SEQ ID NO: 6): GAG, P11-26 (SEQ ID NO: 7): GAG, P11-28 (SEQ ID NO: 8) GAG, P11-31 (SEQ ID NO: 9): GAG and or P11-32 (SEQ ID NO:10):GAG. 15. The composition according to claim 1, wherein the peptide capable of self-assembling is a positively charged peptide and is associated with a positively charged polysaccharide selected from the group consisting of chitosan and its derivatives and other cationic polysaccharides. 16. The composition according to claim 1, wherein the peptide capable of self-assembling is negatively charged and is P11-4 (SEQ ID NO:11), P11-9 (SEQ ID NO:12), P11-13 (SEQ ID NO:13), P11-15 (SEQ ID NO:14), P11-17 (SEQ ID NO:15), P11-20 (SEQ ID NO; 16), P11-24 (SEQ ID NO; 17), P11-25 (SEQ ID NO:18), P11-27 (SEQ ID NO:19), P11-29 (SEQ ID NO:20) and or P11-30 (SEQ ID NO:21). 17. The composition according to claim 16 wherein the overall net negative charge on the peptide is −2, −4 or −6. 18. (canceled) 19. A composition according to claim 16, wherein: the amino acid residues in positions 5, 7 and 9 are glutamic acid (E);the amino acid residue at position 3 is glutamic acid (E) or arginine (R);the amino acid residue at position 4 and 8 are the same and are selected from the group consisting of phenylalanine (F), glutamine (Q) and serine (S)the amino acid residue at position 6 is tryptophan (W), glutamine (Q) or serine (S); orthe amino acid residues at positions 1 and 2 on the peptide are the same or different but reversed at positions 10 and 11. 20.-23. (canceled) 24. The composition according to claim 16, wherein the peptide capable of assembling is negatively charged and is associated with a negatively charged polysaccharide selected from the group consisting of glycosaminoglycan (GAG), oligosaccharide, mucopolysaccharide and dextran. 25. The composition according to claim 24, wherein the negatively charged polysaccharide is a glycosaminoglycan (GAG) selected from the group consisting of chondroitin sulfate, dermatan sulfate, kertatan sulfate, hyaluronan, hyaluronic acid, heparin and heparan sulphate. 26. The composition according to claim 16, wherein the self-assembling peptide complex is selected from the group comprising P11-4 (SEQ ID NO: 11): GAG, P11-9 (SEQ ID NO: 12): GAG, P11-13 (SEQ ID NO: 13): GAG, P11-15 (SEQ ID NO: 14): GAG, P11-17 (SEQ ID NO: 15): GAG, P11-20 (SEQ ID NO: 16): GAG, P11-24 (SEQ ID NO: 17): GAG, P11-25 (SEQ ID NO: 18) GAG, P11-27 (SEQ ID NO: 19): GAG, P11-29 (SEQ ID NO:20):GAG and or P11-30 (SEQ ID NO:21):GAG. 27. The composition according to claim 16, wherein the peptide capable of self-assembling is negatively charged and is associated with a positively charged polysaccharide selected from the group consisting of chitosan and its derivatives and other cationic polysaccharides. 28. The composition of claim 1, wherein the self-assembling peptide complex has an elastic modulus in the range 1 to 400,000 Pa. 29. The composition according to claim 1, wherein the peptide and/or self-assembling peptide complex forms ribbons, fibrils, fibres or a three dimensional scaffold in a β-sheet tape-like substructure. 30. The composition according to claim 1, wherein the self-assembling peptide complex is in a gel, fluid, semi-solid, hydrogel, viscous solution, Newtonian fluid solution, aerogel, visco-elastic solid, xerogel, surface coating, film or a non-woven fabric. 31. The composition according to claim 1, wherein the self-assembling peptide complex comprises a peptide capable of self-assembling and a polysaccharide that are not covalently bonded together. 32. A method of preparing a self-assembling peptide complex, comprising: mixing at least one charged peptide capable of self-assembling in solution with a negatively charged polysaccharide in a selected ratio so as to form a gel. 33. The method according to claim 32, wherein the solution is a saline solution. 34. The method according to claim 32, wherein mixing the charged peptide capable of self-assembling and the polysaccharide comprises simultaneous injection into a recipient directly to the point at which it is desired to form a gel in situ. 35. The method according to claim 32, wherein mixing the charged peptide capable of self-assembling comprises injection to an appropriate site before or after injection of the polysaccharide. 36. An injectable biomaterial composition comprising a self-assembling peptide complex, the complex comprising: (i) at least one charged peptide capable of self-assembling having either an overall net positive charge or an overall net negative charge; and(ii) an oppositely or same charged polysaccharide. 37. The biomaterial composition according to claim 36, wherein the composition forms a gel, semi-solid, hydrogel, viscous solution, visco-elastic solid, xerogel, surface coating or film at the site where it has been injected. 38. (canceled) 39. The self-assembling peptide complex of claim 1, further comprising a bioactive peptide sequence attached on either or both of the peptide termini. 40. A personal care products, tissue reconstruction devices, scaffold for tissue engineering or an adjunct for ophthalmology procedures comprising the self-assembling peptide complex of claim 1. 41.-43. (canceled) 44. A method of repairing or replacing or restoring the nucleus pulposus of a disc, comprising administering the self-assembling peptide complex according to claim 1 into an area of the nucleus pulposus of a disc that requires treatment. 45. The method according to claim 42, wherein the self-assembling peptide complex has shear elastic and viscous moduli in the range of about 8-12 kPa for G′ and about 3.5 to 5.5 kPa for G″. 46. (canceled) 47. A kit comprising; a charged polysaccharide,at least one peptide capable of self-assembling selected from the group comprising any one of SEQ ID NOs 1 to 21; andmeans for combining the polysaccharide and the peptide(s), wherein upon combination of the polysaccharide and the peptide a peptide complex is formed that has an elastic modulus in the range 1 to 400,000 Pa, the kit optionally further including a set of written instructions. 48. the kit according to claim 47, wherein the means for combining the polysaccharide and the peptide(s) is a syringe comprising two barrels, wherein a first barrel comprises the polysaccharide and a second barrel comprises the peptide and the first and the second barrels are adapted to be in fluid communication when the peptide and polysaccharide are ejected from the syringe to allow mixing of the peptide and polysaccharide. 49. (canceled)
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