METHOD FOR CULTURING MESENCHYMAL STEM CELLS ACCORDING TO CELL SIZE
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IPC분류정보
국가/구분
United States(US) Patent
공개
국제특허분류(IPC7판)
C12N-005/0775
A61K-035/28
출원번호
US-0915680
(2014-08-29)
공개번호
US-0222353
(2016-08-04)
우선권정보
KR-10-2013-0106673 (2013-09-05)
국제출원번호
PCT/KR2014/008079
(2014-08-29)
발명자
/ 주소
YANG, Yoon-Sun
OH, Won II
JIN, Hye Jin
KWON, Soon-Jae
KIM, Miyeon
출원인 / 주소
MEDIPOST CO., LTD.
인용정보
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초록▼
A method for culturing mesenchymal stem cells. The method includes isolating mesenchymal stem cells having a size of 8 μm or less and culturing the mesenchymal stem cells in a medium containing calcium and magnesium and under a low oxygen condition. The method can significantly improve the prolifera
A method for culturing mesenchymal stem cells. The method includes isolating mesenchymal stem cells having a size of 8 μm or less and culturing the mesenchymal stem cells in a medium containing calcium and magnesium and under a low oxygen condition. The method can significantly improve the proliferative capacity and differentiation potential of the mesenchymal stem cells.
대표청구항▼
1. A method for culturing mesenchymal stem cells, comprising the steps of: (1) isolating mesenchymal stem cells having a size of 8 μm or less; and(2) culturing the mesenchymal stem cells in a medium containing 2.1 to 3.8 mM of calcium and 1.0 to 3.0 mM of magnesium under a condition of 2 to 5% oxyge
1. A method for culturing mesenchymal stem cells, comprising the steps of: (1) isolating mesenchymal stem cells having a size of 8 μm or less; and(2) culturing the mesenchymal stem cells in a medium containing 2.1 to 3.8 mM of calcium and 1.0 to 3.0 mM of magnesium under a condition of 2 to 5% oxygen. 2. The method of claim 1, wherein the mesenchymal stem cells are derived from umbilical cord blood, bone marrow, lipid, muscle, skin, amniotic fluid, umbilical cord, or tooth. 3. The method of claim 1, wherein the isolation of the mesenchymal stem cells of the step (1) is performed by passing the the mesenchymal stem cells through a filtration membrane having a pore size of 8 μm. 4. The method of claim 1, wherein the medium is selected from the group consisting of Dulbecco's modified eagle medium (DMEM), minimal essential medium (MEM), α-MEM, McCoys 5 A medium, eagle's basal medium, Connaught Medical Research Laboratory (CMRL) medium, Glasgow MEM, Ham's F-12 medium, Iscove's modified Dulbecco's medium (IMDM), Leibovitz's L-15 medium, Roswell Park Memorial Institute (RPMI) 1640 medium, medium 199, and Hank's medium 199. 5. The method of claim 4, wherein the medium comprises 5 to 30% of fetal bovine serum. 6. The method of claim 4, wherein the medium does not comprise fetal bovine serum but a serum replacement. 7. The method of claim 1, wherein the medium is based on an α-MEM supplemented with 5 to 30% of fetal bovine serum, 0.3 to 2.0 mM of calcium, and 0.2 to 2.2 mM of magnesium. 8. The method of claim 1, wherein the cultured mesenchymal stem cells are sub-cultured under the same culture conditions as described in claim 1. 9. Mesenchymal stem cells obtained by the method of claim 1. 10. A cell therapeutic agent comprising the mesenchymal stem cells of claim 9. 11. The cell therapeutic agent of claim 10, which is used for regeneration or protection of adipocytes, osteocytes, chondrocytes, myocytes, neurocytes, cardiomyocytes, hepatocytes, islet beta cells, vascular cells, or pneumocytes. 12. The cell therapeutic agent of claim 10, which is used for one selected from the group consisting of treatment of pulmonary diseases; suppression or treatment of lung disease-induced inflammation; regeneration of pulmonary tissues; and suppression of pulmonary fibrosis. 13. The cell therapeutic agent of claim 10, which is used for regeneration of cartilage. 14. The cell therapeutic agent of claim 10, which is used for therapy of autoimmune diseases or graft-vs-host diseases. 15. A method for improving proliferative capacity and differentiation potential of mesenchymal stem cells, comprising the steps of: (1) isolating mesenchymal stem cells having a size of 8 μm or less; and(2) culturing the isolated mesenchymal stem cells in a medium containing 2.1 to 3.8 mM of calcium and 1.0 to 3.0 mM of magnesium under a condition of 2 to 5% oxygen.
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