Methods and Compositions for Hematoxylin and Eosin Staining
원문보기
IPC분류정보
국가/구분
United States(US) Patent
공개
국제특허분류(IPC7판)
G01N-001/30
G01N-033/68
출원번호
US-0216910
(2016-07-22)
공개번호
US-0327458
(2016-11-10)
발명자
/ 주소
Hansen, Stanley E.
Myers, Russell
Paul, Ronald
Rasmussen, Audra
Taylor, Kate
출원인 / 주소
Hansen, Stanley E.
인용정보
피인용 횟수 :
0인용 특허 :
0
초록▼
The present invention provide for solutions of a defined composition useful in a staining protocol, such as a hematoxylin and eosin staining protocol, when used at certain points of the staining protocol. The formulations of these defined solutions are such that carry-over of the solutions will not
The present invention provide for solutions of a defined composition useful in a staining protocol, such as a hematoxylin and eosin staining protocol, when used at certain points of the staining protocol. The formulations of these defined solutions are such that carry-over of the solutions will not negatively impact, or preferably, will stabilize or favorably modify staining reagent solutions coming in contact with the solutions. In certain embodiments of the invention, solutions are buffered to maintain a specific pH that when carried-over—such as carried-over into hematoxylin—will not significantly influence the pH of the next staining reagent.
대표청구항▼
1. A pH buffered solution comprising an organic acid and a polyhydroxy alcohol, wherein the pH buffered solution has a pH buffering range that comprises from about pH 2.0 to about pH 5.0. 2. The pH buffered solution of claim 1 wherein the pH buffered solution has a pH buffering range of from about p
1. A pH buffered solution comprising an organic acid and a polyhydroxy alcohol, wherein the pH buffered solution has a pH buffering range that comprises from about pH 2.0 to about pH 5.0. 2. The pH buffered solution of claim 1 wherein the pH buffered solution has a pH buffering range of from about pH 2.0 to about pH 5.0. 3. The pH buffered solution of claim 1 wherein the pH buffered solution comprises from about 5% to about 30% (v/v) of a polyhydroxy alcohol selected from the group consisting of ethylene glycol, propylene glycol, glycerin, and polyethylene glycol. 4. The pH buffered solution of claim 1 wherein the pH buffered solution comprises from about 5% to about 30% (v/v) of ethylene glycol. 5. The pH buffered solution of claim 1, wherein the organic acid is selected from the group consisting of malic acid, citric acid, maleic acid, acetic acid, and tartaric acid. 6. The pH buffered solution of claim 1, wherein the organic acid is selected from the group consisting of malic acid, citric acid, and tartaric acid. 7. The pH buffered solution of claim 1, wherein the organic acid is tartaric acid. 8. The pH buffered solution of claim 1, wherein the pH buffered solution comprises a concentration of from about 10 mM to about 100 mM of the organic acid. 9. The pH buffered solution of claim 1, wherein the pH buffered solution is buffered such that the pH is maintained between about pH 2.0 to about pH 5.0 when water is introduced into the solution. 10. The pH buffered solution of claim 1 wherein the pH buffered solution comprises from about 10 mM to about 100 mM of tartaric acid and from about 5% to about 30% (v/v) of ethylene glycol. 11. The pH buffered solution of claim 1 further comprising an antimicrobial agent that is effective over at least a pH range of from about pH 2.0 to about pH 5.0. 12. A solution with a pH of from about pH 2.0 to about pH 5.0 comprising aluminum salts and from about 5% to about 30% (v/v) of a polyhydroxy alcohol selected from the group consisting of ethylene glycol, propylene glycol, glycerin, and polyethylene glycol. 13. The solution of claim 12 wherein the aluminum salts are selected from the group comprising aluminum ammonium sulfate, aluminum sulfate, and aluminum potassium sulfate. 14-19. (canceled) 20. A kit for performing hematoxylin and eosin staining, the kit comprising a pH buffered solution of claim 1 and at least one hematoxylin and eosin staining reagent selected from the group consisting of a hematoxylin solution, an eosin solution, a differentiating solution, and a bluing agent solution. 21. The kit of claim 20 comprising (i) a pH buffered solution of claim 1, (ii) a hematoxylin solution, (iii) an eosin solution, (iv) a differentiating solution, and (v) a bluing agent solution. 22. The kit of claim 20 wherein the pH buffered solution has a pH buffering range of from about pH 2.0 to about pH 5.0. 23. The kit of claim 20 wherein the pH buffered solution comprises from about 5% to about 30% (v/v) of a polyhydroxy alcohol selected from the group consisting of ethylene glycol, propylene glycol, glycerin, and polyethylene glycol. 24. The kit of claim 20 wherein the pH buffered solution comprises from about 5% to about 30% (v/v) of ethylene glycol. 25. The kit of claim 20, wherein the organic acid is selected from the group consisting of malic acid, citric acid, maleic acid, acetic acid, and tartaric acid. 26. The kit of claim 20, wherein the organic acid is selected from the group consisting of malic acid, citric acid, and tartaric acid. 27. The kit of claim 20, wherein the organic acid is tartaric acid. 28. The kit of claim 20, wherein the pH buffered solution comprises a concentration of from about 10 mM to about 100 mM of the organic acid. 29. The kit of claim 20, wherein the pH buffered solution is buffered such that the pH is maintained between about pH 2.0 to about pH 5.0 when water is introduced into the solution. 30. The kit of claim 20 wherein the pH buffered solution comprises from about 10 mM to about 100 mM of tartaric acid and from about 5% to about 30% (v/v) of ethylene glycol.
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