[미국특허]
METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE FAMILY ENTEROBACTERIACEAE HAVING AN ATTENUATED EXPRESSION OF A gshA GENE
원문보기
IPC분류정보
국가/구분
United States(US) Patent
공개
국제특허분류(IPC7판)
C12N-009/00
C12P-013/24
C12P-013/08
C12N-015/52
C12P-013/12
C12P-013/14
C12P-013/10
C12P-013/22
C12P-013/06
출원번호
US-0165606
(2016-05-26)
공개번호
US-0348089
(2016-12-01)
우선권정보
RU-2015120052 (2015-05-28)
발명자
/ 주소
Samsonov, Valery Vasilievich
Eremina, Natalia Sergeevna
Stoynova, Natalia Viktorovna
출원인 / 주소
AJINOMOTO CO., INC.
인용정보
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0인용 특허 :
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초록▼
The present invention provides a method for producing an L-amino acid such as a branched-chain L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the gshA gen
The present invention provides a method for producing an L-amino acid such as a branched-chain L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the gshA gene.
대표청구항▼
1. A method for producing an L-amino acid comprising: (i) cultivating an L-amino acid-producing bacterium of the family Enterobacteriaceae in a culture medium to produce the L-amino acid in the culture medium, the bacterium, or both; and(ii) collecting said L-amino acid from the culture medium, the
1. A method for producing an L-amino acid comprising: (i) cultivating an L-amino acid-producing bacterium of the family Enterobacteriaceae in a culture medium to produce the L-amino acid in the culture medium, the bacterium, or both; and(ii) collecting said L-amino acid from the culture medium, the bacterium, or both,wherein said bacterium has been modified to attenuate expression of a gshA gene. 2. The method according to claim 1, wherein said bacterium belongs to the genus Escherichia. 3. The method according to claim 2, wherein said bacterium is Escherichia coli. 4. The method according to claim 1, wherein said bacterium belongs to the genus Pantoea. 5. The method according to claim 4, wherein said bacterium is Pantoea ananatis. 6. The method according to claim 1, wherein said expression of the gshA gene is attenuated by inactivation of the gshA gene. 7. The method according to claim 6, wherein said gshA gene is deleted. 8. The method according to claim 1, wherein said gshA gene is selected from the group consisting of: (A) a DNA comprising the nucleotide sequence of SEQ ID NO: 1;(B) a DNA comprising a variant nucleotide sequence of SEQ ID NO: 1 due to the degeneracy of the genetic code;(C) a DNA having an identity of not less than 60% with respect to the entire nucleotide sequence of SEQ ID NO: 1, and wherein said DNA encodes a protein having an activity of γ-glutamate-cysteine ligase;(D) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 2; and(E) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 2, but which includes one or more mutations comprising substitution, deletion, insertion, or addition of one or several amino acid residues, and wherein said protein has the activity of γ-glutamate-cysteine ligase.(F) a DNA encoding a protein having an identity of not less than 65% with respect to the entire amino acid sequence of SEQ ID NO: 2, wherein said protein has the activity of γ-glutamate-cysteine ligase. 9. The method according to claim 1, wherein said L-amino acid is selected from the group consisting of an aromatic L-amino acid and a non-aromatic L-amino acid. 10. The method according to claim 9, wherein said aromatic L-amino acid is selected from the group consisting of L-phenylalanine, L-tryptophan, and L-tyrosine. 11. The method according to claim 9, wherein said non-aromatic L-amino acid is selected from the group consisting of L-alanine, L-arginine, L-asparagine, L-aspartic acid, L-citrulline, L-cysteine, L-glutamic acid, L-glutamine, glycine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-ornithine, L-proline, L-serine, L-threonine, and L-valine. 12. The method according to claim 1, wherein said L-amino acid is a branched-chain L-amino acid. 13. The method according to claim 12, wherein said branched-chain L-amino acid is selected from the group consisting of L-isoleucine, L-leucine, and L-valine. 14. The method according to claim 13, wherein said L-amino acid is L-valine.
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