BIOSENSOR USING MAGNETIC PARTICLES FOR PATHOGEN DETECTION
원문보기
IPC분류정보
국가/구분
United States(US) Patent
공개
국제특허분류(IPC7판)
G01N-033/543
G01N-033/569
출원번호
US-0867353
(2015-09-28)
공개번호
US-0038373
(2017-02-09)
발명자
/ 주소
ZOUROB, MOHAMMED
출원인 / 주소
Alfaisal University
인용정보
피인용 횟수 :
0인용 특허 :
0
초록▼
There is provided a biosensor for detecting pathogens in a sample. The detection is based on colorimetry. The biosensor comprises one or more particle supports and a magnetic material attached to a planar support. The biosensor embodies magnetic particles that are functionalized using a chemical sub
There is provided a biosensor for detecting pathogens in a sample. The detection is based on colorimetry. The biosensor comprises one or more particle supports and a magnetic material attached to a planar support. The biosensor embodies magnetic particles that are functionalized using a chemical substrate specific to the pathogens to be detected. The sensor may allow for a simultaneous detection of a plurality of pathogens in the sample. Also, the sensor may be disposable. Moreover, the sensor may be integrated in a portable detection device.
대표청구항▼
1. A biosensor for detecting a pathogen in a sample, comprising: a planar support;a particle support attached to a surface of the planar support, the particle support being of a first color;a magnetic material attached to the other surface of the planar support and spaced apart from the location of
1. A biosensor for detecting a pathogen in a sample, comprising: a planar support;a particle support attached to a surface of the planar support, the particle support being of a first color;a magnetic material attached to the other surface of the planar support and spaced apart from the location of the particle support; anda plurality of magnetic particles of a second color different from the first color, each particle being attached to the particle support through a chemical substrate specific to the pathogen to be detected, a first end of the chemical substrate being attached to the magnetic particle and a second end of the chemical substrate being attached to particle support, such that a visual inspection of the particle support reveals only the second color, the first color being masked by the magnetic particles, wherein upon contacting the particle support with the sample, a reaction occurs between the chemical substrate and the pathogen which results in the magnetic particle being released from the particle support revealing the second color, and the released magnetic particle is attracted by the magnetic material. 2. The sensor of claim 1, wherein the magnetic particles are nanoparticles. 3. The sensor of claim 1, wherein the planar support is of plastic material, or is dielectric, or is a piece of paper, or is a piece of cloth. 4. The sensor of claim 1, wherein the particle support is of metal material, or of plastic material, or is dielectric, or is a piece of paper, or is a piece of cloth. 5. The sensor of claim 1, comprising two or more particle supports. 6. The sensor of claim 1, wherein the chemical substrate is a peptide substrate that may be cleaved by the pathogen. 7. The sensor of claim 1, further comprising a first linker between the first end of the chemical substrate and the magnetic particle and a second linked between the second end of the chemical substrate and the particle support, the first and second linkers being the same or different. 8. The sensor of claim 1, comprising two or more particle supports, of the same color or of different colors. 9. The sensor of claim 1, comprising first and second particle supports, wherein a first set of magnetic particles attached to the first particle support is of a color, and a second set of magnetic particles attached to the second particle support is of another color. 10. The sensor of claim 1, comprising first and second particle supports, wherein a first chemical substrate attached to the first particle support is specific to a pathogen, and a second chemical substrate attached to the second particle support is specific to another pathogen. 11. The sensor of claim 1, wherein the pathogen is selected from the group consisting of Pseudomonas aeruginosa, Staphyloccocus aureus, Escherichia coli, Porphyromonas gingivalis and Listeria monocytogens. 12. The sensor of claim 1, wherein the sample is of biological origin, of clinical origin, from a patient, from food, from a chemical plant, from an industrial plant, from a hospital, from a school or from the environment. 13. The sensor of claim 1, which is disposable. 14. A biosensor for detecting a pathogen in a sample, comprising: a planar support;a golden strip attached to a surface of the planar support;a magnetic material attached to the other surface of the planar support and spaced apart from the location of the golden strip; anda plurality of colored magnetic nanoparticles, each particle being attached to the golden strip through a chemical substrate specific to the pathogen to be detected, a first end of the chemical substrate being attached to the colored magnetic nanoparticle and a second end of the chemical substrate being attached to golden strip, such that a visual inspection of the golden strip reveals only the color of the nanoparticles, wherein upon contacting the golden strip with the sample, a reaction occurs between the chemical substrate and the pathogen which results in the colored magnetic nanoparticle being released from the golden strip revealing the golden color, and the released colored magnetic particle is attracted by the magnetic material. 15. A method of detecting a pathogen in a sample, comprising: (a) providing a planar support comprising a particle support of a first color attached to a surface thereof and a magnetic material attached to the other surface thereof spaced apart from the location of the metal strip;(b) providing a plurality of magnetic particles of a second color different from the first color;(c) providing a chemical substrate specific to the pathogen to be detected, the chemical substrate having first and second ends;(d) attaching the first end of the chemical substrate to the magnetic particles to obtain functionalized magnetic particles;(e) attaching the functionalized magnetic particles to the particle support through the second end of chemical substrate to obtain a functionalized particle support, such that a visual inspection of the functionalized particle support reveals only the second color, the first color being masked by the functionalized magnetic particles; and(f) contacting the functionalized particle support with the sample, wherein, if the pathogen is present in the sample, a reaction occurs between the chemical substrate and the pathogen which results in the magnetic particle being released from the particle support revealing the second color, and the released magnetic particle is attracted by the magnetic material, and if the pathogen is absent, no reaction occurs and no color change is observed. 16. The method of claim 15, further comprising a washing step after step (d) to remove any unattached chemical substrate. 17. The method of claim 15, further comprising, after step (e), a step of passing a magnet above the particle support to remove any unattached functionalized magnetic carrier. 18. The method of claim 15, wherein step (f) comprises dropping the sample on the functionalized particle support, or swabbing the sample with the functionalized particle support, or delivering the sample on to the functionalized particle support by capillary action or use of a micro-fluid.
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