Novel Cell Penetrating Peptide, Conjugate Thereof with Botulinum Toxin, and Use Thereof
원문보기
IPC분류정보
국가/구분
United States(US) Patent
공개
국제특허분류(IPC7판)
A61K-038/48
C12N-009/52
A61K-009/00
C07K-007/08
A61K-038/10
출원번호
US-0313259
(2015-05-29)
공개번호
US-0246266
(2017-08-31)
국제출원번호
PCT/KR2015/005434
(2015-05-29)
발명자
/ 주소
Lee, Byung Kyu
Lee, Kang Jin
Kim, Min Joong
Park, Hong Gyu
출원인 / 주소
Lee, Byung Kyu
인용정보
피인용 횟수 :
0인용 특허 :
0
초록▼
The present invention relates to: a novel cell penetrating peptide; a cell penetrating botulinum toxin recombinant protein composition in which the cell penetrating peptide and the light chain of a botulinum toxin are fused; and a use thereof and, more specifically, to a composition enabling the tra
The present invention relates to: a novel cell penetrating peptide; a cell penetrating botulinum toxin recombinant protein composition in which the cell penetrating peptide and the light chain of a botulinum toxin are fused; and a use thereof and, more specifically, to a composition enabling the transdermal delivery of a cell penetrating botulinum toxin recombinant protein and capable of being locally used for various treatments of the skin and cosmetic purposes. The cell penetrating peptide-botulinum toxin recombinant protein of the present invention can be transdermally delivered, thereby having the intrinsic effect of a botulinum toxin and simultaneously having greater convenience of use, and thus can be effectively applied as a local agonist for the treatment of various diseases and aesthetic and/or cosmetic purposes.
대표청구항▼
1. A cell-penetrating peptide, which is a peptide capable of mediating the delivery of a biologically active molecule into cells, the peptide consisting of an amino acid sequence of SEQ. ID. NO: 1. 2. A polynucleotide encoding the peptide of claim 1. 3. The polynucleotide of claim 2, wherein the pol
1. A cell-penetrating peptide, which is a peptide capable of mediating the delivery of a biologically active molecule into cells, the peptide consisting of an amino acid sequence of SEQ. ID. NO: 1. 2. A polynucleotide encoding the peptide of claim 1. 3. The polynucleotide of claim 2, wherein the polynucleotide consists of a nucleotide sequence of SEQ. ID. NO: 2. 4. A cell-penetrating botulinum toxin recombinant protein in which a cell-penetrating peptide consisting of an amino acid sequence of SEQ. ID. NO: 1 is conjugated with one or both termini of the light chain of botulinum toxin. 5. The cell-penetrating botulinum toxin recombinant protein of claim 4, wherein the botulinum toxin recombinant protein consists of an amino acid sequence selected from the group consisting of SEQ. ID. NO: 31 to SEQ. ID. NO: 58. 6. The cell-penetrating botulinum toxin recombinant protein of claim 4, wherein the light chain of botulinum toxin consists of an amino acid selected from the group consisting of SEQ. ID. NO: 3 to SEQ. ID. NO: 9. 7. The cell-penetrating botulinum toxin recombinant protein of claim 4, wherein the light chain of botulinum toxin further comprises a hexahistidine tag at one terminus. 8. The cell-penetrating botulinum toxin recombinant protein of claim 4, wherein the light chain of botulinum toxin is selected from the group consisting of botulinum toxin serotypes A, B, C, D, E, F and G. 9. The cell-penetrating botulinum toxin recombinant protein of claim 4, wherein the conjugation is conjugation of the cell-penetrating peptide to a carboxyl terminus or an amino terminus of the light chain of botulinum toxin, or both thereof. 10. The cell-penetrating botulinum toxin recombinant protein of claim 4, wherein the conjugation is created by a peptide bond or a covalent bond. 11. A polynucleotide encoding the cell-penetrating botulinum toxin recombinant protein of claim 4. 12. The polynucleotide of claim 11, wherein the polynucleotide consists of a nucleotide sequence selected from the group consisting of SEQ. ID. NO: 59 to SEQ. ID. NO: 86. 13. A recombinant expression vector comprising the polynucleotide of claim 11. 14. The recombinant expression vector of claim 13, wherein the recombinant expression vector comprises an affinity tag selected from the group consisting of His, HAT, FLAG, c-myc, SBP, a chitin-conjugated domain, glutathione-S transferase and a maltose-conjugated protein. 15. A bacterium transformed with the recombinant expression vector of claim 13. 16-20. (canceled) 21. A method for treating a disease selected from the group consisting of facial spasms, eyelid spasms, torticollis, blepharospasm, cervical dystonia, oropharynx dystonia, spasmodic dysphonia, migraines, pruritis ani and hyperhidrosis, the method comprising: transdermally administering the cell-penetrating botulinum toxin recombinant protein of claim 4 into a subject. 22. A method for improving wrinkles, a square jaw and a sharp jaw, injuries, skin softening, scars, acne, pores, elasticity or keloids, the method comprising: transdermally administering the cell-penetrating botulinum toxin recombinant protein of claim 4 into a subject. 23. A method for producing a cell-penetrating botulinum toxin recombinant protein, comprising: culturing the transformed bacteria of claim 15.
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