The invention is directed to a method for producing an edible composition, comprising incubating a three-dimensional porous scaffold and a plurality of cell types comprising: myoblasts or progenitor cells thereof, at least one type of extracellular (ECM)-secreting cell and endothelial cells or proge
The invention is directed to a method for producing an edible composition, comprising incubating a three-dimensional porous scaffold and a plurality of cell types comprising: myoblasts or progenitor cells thereof, at least one type of extracellular (ECM)-secreting cell and endothelial cells or progenitor cells thereof, and inducing myoblasts differentiation into myotubes.
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1.-18. (canceled) 19. A composition comprising: a. a three-dimensional porous scaffold;b. myotubes comprising 10,000-250,000 myotube nuclei per mm3 of said three-dimensional porous scaffold; andc. a plurality of cell types selected from the group consisting of: (i) myoblasts or progenitor cells ther
1.-18. (canceled) 19. A composition comprising: a. a three-dimensional porous scaffold;b. myotubes comprising 10,000-250,000 myotube nuclei per mm3 of said three-dimensional porous scaffold; andc. a plurality of cell types selected from the group consisting of: (i) myoblasts or progenitor cells thereof; and at least one of: (ii) at least one type of ECM-secreting cell; or (iii) endothelial cells or progenitor cells thereof, wherein said endothelial cells or progenitor cells thereof comprise less than 15% of said plurality of cells. 20. The composition of claim 19, wherein said plurality of cell types comprises myoblasts or progenitor cells thereof, at least one type of extracellular matrix (ECM)-secreting cells, and endothelial cells or progenitor cells thereof. 21. The composition of claim 19, wherein said a progenitor cell of a myoblast is a satellite cell. 22. The composition of claim 19, wherein said plurality of cell types comprises satellite cells, ECM-secreting cells, and endothelial cells. 23. The composition of claim 19, wherein said ECM-secreting cell is a fibroblast, a progenitor cell thereof, or a combination thereof. 24. The composition of claim 19, wherein said three-dimensional porous scaffold is selected from the group consisting of: a textured protein, a non-textured protein, and a polysaccharide. 25. The composition of claim 24, wherein said textured protein is textured soy protein. 26. The composition of claim 19, wherein said three dimensional porous scaffold comprises pores with an average diameter ranging from 20 to 1,000 micrometers. 27. The composition of claim 19, wherein said plurality of cell types are non-human cells. 28. The composition of claim 19, wherein said plurality of cells are derived from a livestock mammal. 29. The composition of claim 19, wherein said three-dimensional porous scaffold further comprises an extracellular matrix. 30. The composition of claim 19, wherein said composition is edible. 31. A method for producing the composition of claim 19, the method comprising the steps of: a. incubating a three-dimensional porous scaffold and a plurality of cell types comprising: (i) myoblasts or progenitor cells thereof; and at least one of: (ii) at least one type of extracellular matrix (ECM)-secreting cell; or (iii) endothelial cells or progenitor cells thereof, wherein said myoblasts or progenitor cells thereof and said endothelial cells or progenitor cells thereof are incubated at a ratio ranging between 10:1 to 1:10; andb. inducing differentiation of myoblasts or progenitor cells thereof into myotubes, thereby producing the composition. 32. The method of claim 31, wherein said plurality of cell types comprises myoblasts or progenitor cells thereof, at least one type of extracellular matrix (ECM)-secreting cell, and endothelial cells or progenitor cells thereof. 33. The method of claim 31, wherein said plurality of cell types comprises satellite cells, ECM-secreting cells, and endothelial cells. 34. The method of claim 31, wherein said myoblasts or progenitor cells thereof and ECM-secreting cells are incubated at a ratio ranging between 10:1 to 1:1. 35. The method of claim 31, wherein said ECM-secreting cells and endothelial cells are incubated at a ratio ranging between 1:10 to 1:1. 36. The method of claim 31, wherein said satellite cells, said ECM-secreting cells, and said endothelial cells are incubated at a ratio ranging between 10:1:1 to 2:1:10. 37. The method of claim 31, wherein said plurality of cells are derived from a livestock mammal. 38. The method of claim 31, wherein said myoblasts or progenitor cells thereof and said three-dimensional porous scaffold are incubated at a ratio ranging from 103 to 107 of said myoblasts or progenitor cells thereof to 10 mg of said three-dimensional porous scaffold.
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