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Compositions, methods, and kits for stable Trypan Blue solutions are provided. In one example, a method includes: cooling a solution of Trypan Blue; and filtering the cooled solution. In another example, a method includes: mixing a water-soluble polymer with a Trypan Blue solution. The method may fu

Compositions, methods, and kits for stable Trypan Blue solutions are provided. In one example, a method includes: cooling a solution of Trypan Blue; and filtering the cooled solution. In another example, a method includes: mixing a water-soluble polymer with a Trypan Blue solution. The method may further include adding one or more ingredients such as an aqueous buffer, an osmolyte, an acid, a base, a buffer, a cell culture medium, water, or combinations thereof.

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1. A Trypan Blue solution comprising: Trypan Blue, an osmolyte, and an aqueous buffer, wherein the osmolyte is not a sodium salt. 2. The Trypan Blue solution of claim 1, wherein the osmolyte is selected from lithium chloride, D-glucose, L-glycine, or any combinations thereof. 3. The Trypan Blue solu

1. A Trypan Blue solution comprising: Trypan Blue, an osmolyte, and an aqueous buffer, wherein the osmolyte is not a sodium salt. 2. The Trypan Blue solution of claim 1, wherein the osmolyte is selected from lithium chloride, D-glucose, L-glycine, or any combinations thereof. 3. The Trypan Blue solution of claim 1 or 2, further comprising an acid or a base. 4. The Trypan Blue solution of claim 3, wherein the acid comprises phosphoric acid and the base comprises alkali hydroxide. 5. The Trypan Blue solution of any one of claims 1-4, wherein the Trypan Blue solution has a pH from about 6.5 to about 8. 6. The Trypan Blue solution of any one of claims 1-5, wherein the buffer is sodium-azide preserved PBS. 7. The Trypan Blue solution of any one of claims 1-5, further comprising adding a water-soluble polymer to the Trypan Blue solution, wherein the water-soluble polymer is selected from the group of: poly(acrylic acid) or a salt thereof, poly(styrene sulfonate) or a salt thereof, poly(vinyl pyrrolidone), or any combinations thereof. 8. The Trypan Blue solution of claim 7, wherein the Trypan Blue solution has a molar ratio of repeating unit of the water-soluble polymer to Trypan Blue from about 1 to about 55. 9. A method for preparing a stable Trypan Blue solution, the method comprising: cooling a solution of Trypan Blue; and filtering the cooled solution. 10. The method of claim 9, further comprising filtering the solution of Trypan Blue at ambient temperature and pressure before the cooling. 11. The method of any one of claims 9-10, wherein the solution comprises sodium-azide preserved phosphate buffered saline. 12. The method of any one of claims 9-11, wherein the solution of Trypan Blue is cooled to a temperature less than about 15° C. 13. The method of any one of claims 9-12, wherein the solution of Trypan Blue is cooled to a temperature less than about 5° C. 14. The method of any one of claims 9-13, wherein the solution of Trypan Blue is cooled a temperature of about 0° C. to about 14° C. 15. The method of any one of claims 9-14, wherein the Trypan Blue is at a concentration near, at, or beyond the solubility limit in the solution. 16. The method of any one of claims 9-15, wherein solution of Trypan Blue is made by mixing Trypan Blue with an aqueous buffer at ambient or near-ambient temperature and pressure before cooling. 17. The method of any one of claims 9-16, further comprising adding a water-soluble polymer to the Trypan Blue solution prior to cooling and/or after filtration, wherein the water-soluble polymer is selected from the group of: poly(acrylic acid) or a salt thereof, poly(styrene sulfonate) or a salt thereof, poly(vinyl pyrrolidone), or any combinations thereof. 18. The method of claim 17, wherein the Trypan Blue solution has a molar ratio of repeating unit of the water-soluble polymer to Trypan Blue from about 1 to about 55. 19. The method of any one of claims 9-18, further comprising storing the filtered Trypan Blue solution at a temperature from about 0° C. and 25° C. 20. The method of any one of claims 9-19, wherein the filtered Trypan Blue solution is free or substantially free of precipitated impurities after storing for at least about 10 minutes at a temperature of about 15° C. to about 30° C. 21. A Trypan Blue solution comprising: Trypan Blue and a water-soluble polymer. 22. The Trypan Blue solution of claim 21, further comprising an aqueous buffer. 23. The Trypan Blue solution of any one claims 21-22, wherein the water-soluble polymer is selected from the group of: poly(acrylic acid) or a salt thereof, poly(styrene sulfonate) or a salt thereof, poly(vinyl pyrrolidone), or any combinations thereof. 24. The Trypan Blue solution of any one claims 21-23, wherein the Trypan Blue solution has a concentration of water-soluble polymer from about 0.05% to about 4%, based on the total weight (w/w %) or total volume (w/v %) of the Trypan Blue solution. 25. The Trypan Blue solution of any one claims 21-24, wherein the Trypan Blue solution has a repeating unit concentration of the water-soluble polymer from about 5 mmol/L to about 500 mmol/L. 26. The Trypan Blue solution of any one claims 21-25, wherein the Trypan Blue solution has a molar ratio of repeating unit of the water-soluble polymer to Trypan Blue from about 1 to about 55. 27. The Trypan Blue solution of any one claims 21-26, wherein the water-soluble polymer is a poly(acrylic acid) or an alkali salt thereof with an average molecular weight from about 4,000 to about 6,000 g/mol. 28. The Trypan Blue solution of any one claims 21-27, wherein the water-soluble polymer is a poly(4-styrene sulfonate) or an alkali salt thereof with an average molecular weight from about 50,000 to about 80,000 g/mol. 29. A method for making a stable Trypan Blue solution, the method comprising: providing a solution of Trypan Blue; and adding a water-soluble polymer to the solution of Trypan Blue. 30. The method of claim 29, wherein the water-soluble polymer is selected from the group of: poly(acrylic acid) or a salt thereof, poly(styrene sulfonate) or a salt thereof, poly(vinyl pyrrolidone), or any combinations thereof. 31. The method of any one of claims 29-30, further comprising adjusting Trypan Blue concentration to a range from about 0.01% to about 2%, based on the total weight (in the unit of w/w %) or total volume (in the unit w/v %) of the Trypan Blue solution. 32. The method of any one of claims 29-31, further comprising adjusting a molar ratio of repeating unit of the water-soluble polymer to Trypan Blue from about 1 to about 55. 33. The method of any one of claims 29-32, further comprising filtering the Trypan Blue solution before, or after adding the water-soluble polymer.