[특허]Automated radioimmunoassay

Automated radioimmunoassay 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-033/16 G01N-023/12
출원번호	US-0666302 (1976-03-12)
발명자 / 주소	Brooker Gary (Charlottesville VA) Terasaki Wesley L. (Charlottesville VA) Price Michael G. (Charlottesville VA)
출원인 / 주소	The University of Virginia (Charlottesville VA 02)
인용정보	피인용 횟수 : 27 인용 특허 : 0

초록 ▼

An automated radioimmunoassay apparatus which comprises: MEANS FOR TIME CONTROLLED INCUBATION OF A MULTIPLICITY OF SAMPLE SOLUTIONS, EACH OF WHICH COMPRISE A MIXTURE OF (A) A SAMPLE, POSSIBLY CONTAINING AN ANTIGEN, INTENDED TO BE ASSAYED, (B) A SOLUTION OF A KNOWN CONCENTRATION OF AN ANTIGEN TAGGED WITH A RADIOACTIVE ISOTOPE AND (C) A SOLUTION OF A KNOWN TITER OF AN ANTIBODY REACTABLE WITH SAID ANTIGEN; RADIOACTIVITY DETECTOR MEANS FOR PROVIDING A KINETIC MEASUREMENT OF RADIOACTIVITY AS A SOLUTION IS CONTINUOUSLY PASSED THERETHROUGH, AND FOR PROVIDING A STATIC MEASUREMENT OF RADIOACTIVITY AS A SOLUTION IS MAINTAINED STATICALLY WITHIN SAID DETECTOR; MEANS FOR INTRODUCING SAID INCUBATED SAMPLE INTO SAID RADIOACTIVITY DETECTOR MEANS; TIMING MEANS, WHICH IS ACTIVATABLE BY SAID RADIOACTIVITY DETECTOR MEANS WHEN SAID DETECTOR MEANS DETECTS A PREDETERMINED THRESHOLD OF RADIOACTIVITY, FOR MEASURING TIME SEQUENCES AND FOR GENERATING CONTROL SIGNALS AT PREDETERMINED TIME SEQUENCES; RECORDING MEANS COUPLED TO SAID DETECTOR MEANS FOR RECORDING THE AMOUNTS OF RADIOACTIVITY DETECTED IN SAID DETECTOR MEANS; MEANS FOR SEPARATING SAID KINETICALLY MEASURED SOLUTION INTO A FIRST PORTION CONTAINING ANTIGEN WHICH IS UNREACTED WITH SAID ANTIBODY, AND A SECOND PORTION CONTAINING ANTIGEN REACTED WITH SAID ANTIBODY; MEANS FOR INTRODUCING ONE OF SAID PORTIONS FROM SAID SEPARATING MEANS INTO SAID RADIOACTIVITY MEASURING MEANS, FOR STATIC DETECTION; MEANS FOR ISOLATING SAID INCUBATED SAMPLE FROM ADDITIONAL SAMPLE SOLUTIONS INTRODUCED INTO SAID APPARATUS FOR ASSAY, WHEREIN SAID ISOLATING MEANS IS ACTIVATABLE BY A CONTROL SIGNAL EMITTED FROM SAID TIMING MEANS, WHEREBY SAID ISOLATING MEANS ISOLATES THE SYSTEM AT A FIRST PREDETERMINED SIGNAL FROM SAID TIMING MEANS, AND WHEREBY SAID ISOLATING MEANS RECONNECTS THE SYSTEM TO SAID INCUBATION MEANS AT A SECOND PREDETERMINED SIGNAL FROM SAID TIMING MEANS, WHEREIN THE SECOND PREDETERMINED TIME PERIOD IS SET SO AS TO ENABLE SUFFICIENT TIME TO COMPLETE SAID STATIC MEASUREMENT BEFORE THE NEXT INCUBATED SAMPLE ARRIVES IN THE SAID DETECTOR MEANS FOR THE NEXT SEQUENTIAL KINETIC MEASUREMENT; AND MEANS FOR FLUSHING SAID SAMPLE FROM SAID SYSTEM AFTER STATIC MEASUREMENT THEREOF SO AS TO PREPARE SAID DETECTOR FOR THE NEXT SEQUENTIAL SAMPLE FOR STATIC MEASUREMENT.

대표청구항 ▼

A method for continuous radioimmunoassay, which comprises: incubating for a predetermined time period, a multiplicity of sample solutions, each of which comprises a mixture of (a) a sample possibly containing an antigen intended to be assayed, (b) a solution of a known concentration of an antigen tagged with a radioactive isotope, and (c) a solution of a known titer of antisera containing antibodies reactable with said antigen, the concentration of said antibody being selected so as to form an incubated sample containing an antibody-antigen complex, and containing unreacted antigen and tagged antigen, if said sample contains an antigen; directing said incubated sample into a radioactivity detector which is capable of providing a kinetic measurement of radioactivity as the solution is continuously passed therethrough; comparing the amount of radioactivity in said concentrated sample with a predetermined threshold and activating a timing means when said threshold is exceeded; isolating said incubated sample from any additional samples being incubated on a predetermined signal from said timing means; separating said incubated solution into a first portion containing unreacted antigen and tagged antigen and a second portion containing antibody-antigen and antibody-tagged antigen complexes; directing one of said portions into a radioactivity detector, detecting the amount of radioactivity in said portion while said portion is maintained in a virtually static condition, and recording said measurement; evacuating said portion to waste; directing a subsequent incubated sample into said radioactivity detector on a second predetermined signal from said timing means so that the next subsequent incubated sample to be assayed arrives in the radioactivity detector for the next kinetic measurement after the previous static radioactivity measurement is completed; and comparing the ratio of the static radioactivity measurement to the kinetic measurement with a precalibrated ratio to determine the quantity of antigen being assayed.

이 특허를 인용한 특허 (27)

Stoker Ronald L. (Bountiful UT), Assay for ligands by separating bound and free tracer from sample.
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Reese Max (Salt Lake City UT), Automated direct serum radioassay.
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Reese ; Max G., Automated direct serum radioimmunoassay.
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Brooker ; Gary L. ; Terasaki ; Wesley L. ; Price ; Michael G., Automated immunoassay.
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Rapkin, Edward, Chromatography detection system and method.
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Gilmour,Steven B.; Modzelewski,Brent E., Distinguishing test types through spectral analysis.
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Modzelewski, Brent E.; Gilmour, Steven B., Distinguishing test types through spectral analysis.
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Gray Don N. (Sylvania OH) Keyes Melvin H. (Sylvania OH), Enzyme immunoassays using immobilized reagents in a flowing stream.
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Achter, Eugene K.; Schroeder, Hartmut Richard; Trotta, Stephen S., Immunoassay probe.
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Ebersole Richard C. (Newark DE), Immunochemical testing using tagged reagents.
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Tsay Yuh-Geng (San Jose CA) Shah Vipin D. (Saratoga CA), Kinetic radioimmunoassay test method and device.
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Bystrom, Cory E.; Reitz, Richard E.; Clarke, Nigel J., Mass spectrometry assay for plasma-renin.
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Bystrom, Cory E.; Reitz, Richard E.; Clarke, Nigel J., Mass spectrometry assay for plasma-renin.
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Bystrom, Cory E.; Reitz, Richard E.; Clarke, Nigel J., Mass spectrometry assay for plasma-renin.
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Modzelewski, Brent E.; Gilmour, Steven B.; Roth, G. Thomas; Bell, Douglas E., Method for determining concentration of an analyte in a test strip.
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Phillips, Roger; McGarraugh, Geoffery; Jurik, Franklin A.; Underwood, Raymond D., Method for the determination of glucose employing an apparatus emplaced matrix.
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Paolini, Gregory P.; Roth, G. Thomas; Modzelewski, Brent E., Method of strip insertion detection.
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Wang Chia-Gee (Millwood NY), Method of tagged immunoassay.
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Modzelewski, Brent; Rounds, George, Method of using a protective test strip platform for optical meter apparatus.
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Phillips, Roger; McGarraugh, Geoffery; Jurik, Frank; Underwood, Ray, Minimum procedure system for the determination of analytes.
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Brent Modzelewski ; George Rounds, Protective test strip platform.
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Phillips, Roger; McGarraugh, Geoffery; Jurik, Franklin A.; Underwood, Raymond D., Reagents test strip adapted for receiving an unmeasured sample while in use in an apparatus.
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Phillips, Roger; McGarraugh, Geoffery; Jurik, Franklin A.; Underwood, Raymond D., Reagents test strip with alignment notch.
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Phillips Roger ; McGarraugh Geoffery ; Jurik Franklin A. ; Underwood Raymond D., Reflectance measurement of analyte concentration with automatic initiation of timing.
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Wang Chia-Gee (Millwood NY), Tagged immunoassay.
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Talmer, Mark; Limerick, Kathleen; Schroeder, Gary, Tapered cuvette and method of collecting magnetic particles.
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Talmer, Mark; Limerick, Kathleen; Schroeder, Gary, Tapered cuvette and method of collecting magnetic particles.
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