[특허]Test article including a covalently attached diagnostic reagent and method

[미국특허] Test article including a covalently attached diagnostic reagent and method 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-033/16 G01N-021/00
출원번호	US-0663828 (1976-03-04)
발명자 / 주소	Harte Richard A. (Redwood City CA)
출원인 / 주소	International Diagnostic Technology, Inc. (Santa Clara CA 02)
인용정보	피인용 횟수 : 43 인용 특허 : 2

초록 ▼

A body suitable for use in the quantitative detection of an unknown quantity of a biologically derived sample suitably by radioimmunoassay, fluorometric detection, or by spectrophotometry. The body includes a handle attached to a nonparticulate, nonswellable, impermeable continuous surface region. A diagnostic reagent (e.g., an antibody) is covalently attached to the surface region. The surface region is stirred by the handle in a solution of sample substance (e.g., antigen) for reaction with the diagnostic reagent. In a competitive binding technique, a labelled substance (e.g., fluorochrome labelled antigen) is simultaneously contacted with the sample. Thereafter, the surface region is washed, and the label is measured as by a fluorometer. The technique is also applicable to the so-called “sandwich technique”in which the labelled substance (e.g., labelled antibody) is reacted with the sample substance after it has been reacted with the diagnostic reagent. The above type of surface facilitates simple and effective separation of bound from free labelled substance and allows thorough washing to cleanse the surface of residual free and non-specifically bound labelled substance.

대표청구항 ▼

A body suitable for use in the fluorometric detection of a biologically derived sample, said body comprising handle means connected to at least one nonparticulate, nonswellable, impermeable, continuous, flat surface region defining an area of at least one square millimeter and a diagnostic reagent bound to at least one biologically derived sample labelled with a fluorochrome, said diagnostic reagent being covalently attached to said surface region. In a method for the fluorometric detection of an unknown quantity of a biologically derived sample, the steps of: (a) covalently attaching a diagnostic reagent to at least one nonparticulate, nonswellable, impermeable continuous, flat surface region of a body, (b) contacting the reagent-bonded flat surface of step (a) with a sample substance reactive with said diagnostic reagent in solution and with a fluorochrome labelled substance in solution for a sufficient time to form a fluorochrome labelled reaction product capable of detection, (c) separating the reacted surface and solution containing unbound fluorochrome labelled substance after completion of step (b), (d) washing the reacted surface of step (c); and (e) transporting the body to the detection station of a fluorometer to quantitatively measure the amount of fluorescence emitted from the washed reacted surface of step (d).

이 특허에 인용된 특허 (2) 인용/피인용 타임라인 분석

Rubenstein Kenneth Edward (Palo Alto CA) Leute Richard K. (Sunnyvale CA), Antibody steric hindrance immunoassay with two antibodies.
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Blumberg William Emil (Glen Gardner NJ) Eisinger Josef (Bethlehem Township NJ) Lamola Angelo Anthony (Warren NJ), Fluorimetric apparatus and method for analysis of body fluid.
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이 특허를 인용한 특허 (43) 인용/피인용 타임라인 분석

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Hirschfeld Tomas B. (Livermore CA), Apparatus for improving the numerical aperture at the input of a fiber optics device.
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Block Myron J. (334 N. Main St. North Salem NH 03073) Hirschfeld Tomas B. (Livermore CA), Apparatus including optical fiber for fluorescence immunoassay.
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Hirschfeld, Tomas B.; Block, Myron J., Assay apparatus and methods.
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Chandler Howard M.,CAX, Assay device.
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Davis,Paul James; Prior,Michael Evans; May,Keith, Assays.
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Wu, Yuzhang; Yang, Ying; Dai, Jieling; Tung, Hsiaoho Edward, Biological specimen collection and analysis system.
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May Keith,GBX ; Prior Michael Evans,GBX ; Richards Ian,GBX, Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents.
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May Keith,GBX ; Prior Michael Evans,GBX ; Richards Ian,GBX, Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents.
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May, Keith; Prior, Michael Evans; Richards, Ian, Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents.
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Lee Sin H. (53 Milan Rd. Woodbridge CT 06525), Cytochemical agents and methods for the detection of steroid hormone receptors in human tissues.
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Brown Rodney J. (Logan UT) Swaisgood Harold E. (Raleigh NC), Detection of antibiotics in milk.
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Blatt Joel M. ; Mangan Wilma M. ; Patel Paul J. ; Manneh Victor A., Device for preventing assay interference using silver or lead to remove the interferant.
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Margosiak Marion Louise ; Knaggs Helen Elizabeth ; Aronson Michael Paul, Device for the identification of acne, microcomedones, and bacteria on human skin.
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Saaski, Elric W., Enhanced waveguide and method.
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Saaski,Elric W., Enhanced waveguide and method.
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Hirschfeld Tomas E. (Livermore CA), Fluorescent immunoassay employing optical fiber in capillary tube.
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Schall ; Jr. Roy F. (Glendora CA) Lewis Stephen J. (Glendora CA), Glass support coated with synthetic polymer for bioprocess.
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Powers, Linda S.; Fairbanks, Jerrie V.; Roveda, Janet M., High power, sinusoidal LED driver with temperature stabilization feedback and photomultiplier system.
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Chandler Howard M. ; Piasio Roger N. ; Prouty Karen, Immunochromatographic assay device.
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Perry David A. (Portland ME) Stead Peter (Portland ME), Method and apparatus for carrying out solid phase in vitro diagnostic assays.
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Burgett Michael W. (El Granada CA) Harte Richard A. (Redwood City CA), Method of fluoro immunoassay.
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Cheng, Shu-Ching; Huang, Ching; Wu, Ming-Shian; Willrodt, Michael J.; Cunningham, Herbert Bradfield; Fan, Eugene, Method of use of one step immunochromatographic device for A antigen.
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Xie,Zongcen Charles; Pierce,Jeffrey A.; Stivers,Carole R., Methods and systems for point of care bodily fluid analysis.
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Dowben Robert M. (Dallas TX) Bunting James R. (Boston MA), Methods for performing chemical assays using fluorescence and photon counting.
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Saaski,Elric W., Misalignment compensating optical sensor and method.
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Chandler Howard M.,CAX, Opposable-element assay devices, kits, and methods employing them.
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Neel Thomas Gary (Indianapolis IN) Lyst ; Jr. James E. (Indianapolis IN), Optical apparatus for performing an immunoassay.
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Saaski, Elric W.; McCrae, David A., Optical assay apparatus and methods.
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Buckler Robert T. (Edwardsburg MI) Carrico Robert J. (Elkhart IN), Propranolol immunogen and antibodies.
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Kelton Arden A. (Westminster CA) Bell Michael L. (Corona CA) Chung Roy A. (Fountain Valley CA), Rotary fluid manipulator.
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Chiknas Steven G. (111 Wolf Trap Rd. Vienna VA 22180), Rotor or carrier for centrifugal analyzer and bead washer.
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Ramsey, James T.; Hartselle, Larry, Specimen collection and assay container.
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Erb Judith ; Downward ; IV James, Surface treatment and light injection method and apparatus.
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Erb Judith ; Downward ; IV James, Surface treatment and light injection method and apparatus.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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[미국특허] Test article including a covalently attached diagnostic reagent and method 원문보기

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이 특허에 인용된 특허 (2) 인용/피인용 타임라인 분석

이 특허를 인용한 특허 (43) 인용/피인용 타임라인 분석

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[미국특허] Test article including a covalently attached diagnostic reagent and method 원문보기

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이 특허에 인용된 특허 (2) 인용/피인용 타임라인 분석

이 특허를 인용한 특허 (43) 인용/피인용 타임라인 분석

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