[특허]Antienzyme homogeneous competitive binding assay

[미국특허] Antienzyme homogeneous competitive binding assay 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-031/14 G01N-033/16
출원번호	US-0815487 (1977-07-14)
발명자 / 주소	Yoshida Robert A. (Mountain View CA) Maggio Edward T. (Redwood City CA)
출원인 / 주소	Syva Company (Palo Alto CA 02)
인용정보	피인용 횟수 : 19 인용 특허 : 3

초록 ▼

Methods and reagent combinations are provided for competitive protein binding assays for determining a member of an immunological pair (ligand and receptor) whereby an enzyme-ligand conjugate is employed in combination with an enzyme inhibitor, conveniently an antibody to said enzyme. When ligand is the analyte, receptor for ligand is also included in the assay medium, while supplemental amounts of receptor need not be added when receptor is the analyte. The assay is carried out in an aqueous buffered medium, normally at constant temperature, by combining in the assay medium the unknown sample suspected of containing the analyte, enzyme-bound-ligand, ligand receptor (antiligand), enzyme inhibitor (antienzyme), and enzyme substrates, and the enzymatic activity in the assay medium determined. By comparing the observed enzymatic activity with an unknown to the enzymatic activity observed in an assay medium with a known amount of analyte, the amount of analyte can be quantitatively determined. Kits are provided having matched amounts of enzyme-bound-ligand, antienzyme and, when appropriate, antiligand for use in the subject assay.

대표청구항 ▼

A method for determining the presence in a sample of an analyte which is a member of an immunological pair consisting of ligand and ligand receptor which comprises: combining in an aqueous medium: (A) said sample; (B) enzyme-bound-ligand which retains a substantial proportion of its activity when bound to receptor; (C) ligand receptor, when ligand is the analyte, wherein said receptor is capable of specifically binding to said ligand and said enzyme-bound-ligand; and (D) enzyme inhibitor, of at least about 2,000 molecular weight wherein said inhibitor is impeded from inhibiting said enzyme-bound-ligand, when receptor is bound to enzyme-bound-ligand; and determining the enzymatic activity in said medium as compared to the enzymatic activity in a medium having a known amount of analyte.

이 특허에 인용된 특허 (3) 인용/피인용 타임라인 분석

Rubenstein Kenneth Edward (Palo Alto CA) Leute Richard K. (Sunnyvale CA), Antibody steric hindrance immunoassay with two antibodies.
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Ullman Edwin F. (Atherton CA), Double receptor fluorescent immunoassay.
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Bolz Gunner (Santa Clara CA) Deindoerfer Fred H. (Northridge CA) Hu Chun P. (Santa Clara CA) Kameda Naomi (Foster City CA) Wang Robert (San Jose CA), Method for quantitation of antigens or antibodies on a solid surface.
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이 특허를 인용한 특허 (19) 인용/피인용 타임라인 분석

Yoshida Robert A. (San Diego CA), Antienzyme termination in enzyme immunoassays.
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Richter,Mark M.; Powell,Michael J.; Belisle,Christopher M., Assays employing electrochemiluminescent labels and electrochemiluminescence quenchers.
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Liddell John Macdonald,GBX ; Greer William,GBX, Biocatalysts.
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Harpel Peter C. (New York NY), Detecting and distinguishing between plasminogen activators.
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Voss Houston F. (Libertyville IL), Determination of thyroxine binding globulin.
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Edward Benjamin Jakobovits ; Joy L. Silen ; Mark J. Levy ; Thomas C. Goodman ; Martin Becker ; Edwin F. Ullman ; Robert M. Caldwell ; Richard R. Bott ; Christopher Charles Barnett, Homogeneous immunoassays using mutant glucose-6-phosphate dehydrogenases.
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Jakobovits Edward Benjamin ; Silen Joy L. ; Levy Mark J. ; Goodman Thomas C. ; Becker Martin ; Ullman Edwin F. ; Caldwell Robert M. ; Bott Richard R. ; Barnett Christopher Charles, Homogeneous immunoassays using mutant glucose-6-phosphate dehydrogenases.
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Jakobovits Edward Benjamin ; Silen Joy L. ; Levy Mark J. ; Goodman Thomas C. ; Becker Martin ; Ullman Edwin F. ; Caldwell Robert M. ; Bott Richard R. ; Barnett Christopher Charles, Homogeneous immunoassays using mutant glucose-6-phosphate dehydrogenases.
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Sigler Gerald F. ; Rouhani Riaz ; Davoudzadeh David ; Coty William A. ; Shindelman Jeffrey E. ; Morrill Paul R.,GBX, Immunoassay reagents and methods for determination of LSD and LSD metabolites.
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Harpel Peter C. (New York NY), Immunocapture of enzyme inhibitor, enzyme complexes and uses thereof.
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Skold Carl N. (Mountain View CA) Henson Margaret (Mountain View CA) Houts Thomas Michael (Mountain View CA) Gibbons Ian (Portola Valley CA), Method of stabilizing enzyme conjugates.
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Rouhani Riaz ; Sigler Gerald F., Non-hydrolyzable analogs of heroin metabolites suitable for use in immunoassay.
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Smith Richard A. G. (Reigate Surrey GB2), Novel enzyme derivatives.
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Ullman, Edwin F.; Pirio, Marcel; Ericson, Mary C.; Wagner, Daniel B.; Davalian, Dariush, Simultaneous screening of multiple analytes.
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Ullman,Edwin F.; Pirio,Marcel; Ericson,Mary C.; Wagner,Daniel B.; Davalian,Dariush, Simultaneous screening of multiple analytes.
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Ullman,Edwin F.; Pirio,Marcel; Ericson,Mary C.; Wagner,Daniel B.; Davalian,Dariush, Simultaneous screening of multiple analytes.
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Marshall David L. (Carmel IN), Soluble insoluble polymers in enzymeimmunoassay.
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Contestable Paul B. ; Daiss John L. ; Groth Holly L. ; Grogan Elizabeth A. ; Snyder Brian A., Specific binding assay using enzyme inhibitor and anti-inhibitor antibodies.
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Monji Nobuo (Miami Springs FL) Castro Albert (Miami FL), Steric hindrance enzyme immunoassay.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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[미국특허] Antienzyme homogeneous competitive binding assay 원문보기