[특허]Assay employing a labeled Fab-fragment ligand complex

[미국특허] Assay employing a labeled Fab-fragment ligand complex 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-021/00 G01N-031/00 G01N-031/14 G01N-033/16
출원번호	US-0906388 (1978-05-16)
발명자 / 주소	Schwarzberg Moshe (Sunnyvale CA)
출원인 / 주소	Syva Company (Palo Alto CA 02)
인용정보	피인용 횟수 : 44 인용 특허 : 4

초록 ▼

Methods and compositions are provided for improved protein binding assays by preparing compositions having indirectly labeled ligands substantially free of label conjugated to materials other than the indirectly labeled ligand. The method for preparing the compositions involves employing a monovalent receptor to which the label is conjugated and combining the monovalent receptor labeled conjugate to the ligand, either in pure or impure form. The mixture is then segregated according to molecular weight and the ligand conjugated to the labeled receptor isolated. This conjugate may then be directly used as a reagent in a protein binding assay, where the assay mixture is substantially free of label other than labeled receptor bound to ligand. The labels will be for the most part of relatively low molecular weight, while the receptor is preferably a Fab fragment. The ratio of receptor to ligand will be chosen so as to provide reasonable molecular weight distinctions between unbound ligand, unbound labeled receptor, ligand bound to labeled receptor, and other materials which may be in the mixture. Various techniques may be used for the separation. The assays are performed in accordance with known methods employing a second receptor composition, which may be labeled or unlabeled.

대표청구항 ▼

A method for performing a protein binding assay employing a labeled ligand complex reagent, where the ligand is a protein and a member of a specific binding pair consisting of ligand and antiligand and is polyepitopic and of at least about 15,000 molecular weight; and said label is of from about 100 to 2,000 molecular weight and provides a detectible signal affected by the proximity of antiligand due to the steric bulk of said antiligand or a group conjugated to said antiligand which interacts with said label; said labeled ligand complex reagent prepared by the method comprising: (a) combining said ligand with a g100 to 2,000 molecular weight and provides a detectible signal affected by the proximity of antiimunoglobulin due to the steric bulk of said antiimmunoglobulin or a group conjugated to said antiimmunoglobulin which interacts with said label; said labeled immunoglobulin complex reagent prepared by the method comprising: (a) combining said immunoglobulin with a g

이 특허에 인용된 특허 (4) 인용/피인용 타임라인 분석

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이 특허를 인용한 특허 (44) 인용/피인용 타임라인 분석

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[미국특허] Assay employing a labeled Fab-fragment ligand complex 원문보기

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[미국특허] Assay employing a labeled Fab-fragment ligand complex 원문보기

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