[특허]Competitive enzyme-linked immunoassay

[미국특허] Competitive enzyme-linked immunoassay 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-031/14
출원번호	US-0820286 (1977-07-29)
발명자 / 주소	Sasse Edward A. (Brookfield WI) Yorde Donald E. (Milwaukee WI)
출원인 / 주소	The Medical College of Wisconsin, Inc. (Milwaukee WI 02)
인용정보	피인용 횟수 : 26 인용 특허 : 5

초록 ▼

A competitive enzyme-linked immunoassay for a soluble analyte in which the analyte in the unknown sample and the same analyte attached to a solid phase (immunosorbent) compete for a selected quantity of a soluble first antibody to the substance; a second or bridging antibody is then linked to any first antibody linked to the immunosorbent as a result of the competitive reaction, following which a soluble enzyme/antibody immune complex is linked to bridging antibody on the immunosorbent. The enzymatic activity associated with the thusly-formed enzyme-immunosorbent complex or with the non-immunosorbent fraction of enzyme is measured to determine the presence and/or concentration of analyte in the unknown sample.

대표청구항 ▼

A competitive enzyme-linked immunoassay for soluble immunogen or hapten analyte in a sample solution including the steps of: (a) providing an insoluble solid immunosorbent to which analyte has been linked; (b) carrying out a competitive reaction between analyte in the sample solution and analyte linked to said solid immunosorbent for a selected quantity of a first antibody for the analyte; (c) coupling a heterologous bridging second antibody to any first antibody linked to analyte on the solid immunosorbent, the bridging second antibody being directed against the first antibody; (d) coupling a soluble antibody-enzyme immune complex to any bridging second antibody coupled to the first antibody linked to analyte on the immunosorbent, the antibody of the antibody-enzyme immune complex being of the same species as the first antibody or a cross-reactive species; and (e) measuring the activity of the enzyme coupled to the immunosorbent to indirectly determine the presence of the analyte in the sample.

이 특허에 인용된 특허 (5) 인용/피인용 타임라인 분석

Weetall Howard H. (Big Flats NY) Yaverbaum Sidney (Sufferin NY), Detection and quantitation of viral antibodies.
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Weltman Joel K. (4 Wildacre Lane Barrington RI 02806) Rotman M. Boris (11 Mayflower St. Providence RI 02906), Enzyme conjugates.
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Lafontaine George Stanford (Budd Lake NJ), Radioimmunoassay.
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Niswender Gordon Dean (Fort Collins CO), Solid phase double-antibody radioimmunoassay procedure.
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Miles Laughton E. M. (Stanford CA), Universal reagent 2-site immunoradiometric assay using labelled anti (IgG).
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이 특허를 인용한 특허 (26) 인용/피인용 타임라인 분석

Oberbauer, Rainer; Wilflingseder, Julia; Mayer, Bernd; Perco, Paul; Kainz, Alexander, Acute kidney injury risk testing.
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Bieniarz, Christopher; Wong, Jennifer; Lefever, Mark; Kosmeder, II, Jerome W.; Ashworth-Sharpe, Julia; Kernag, Casey A., Antibody conjugates.
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Bieniarz, Christopher; Wong, Jennifer; Lefever, Mark; Kosmeder, II, Jerome W.; Ashworth-Sharpe, Julia; Kernag, Casey A., Antibody conjugates.
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Deutsch Alice (New York NY) Brandwein Harvey (Port Washington NY) Platt Herbert (Great Neck NY) Hunter Dianne M. (New Rochelle NY) Dubitsky Andrew (Amityville NY) Durham Susan M. (Westbury NY), Antigen assay method and kit.
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Lofquist, Alan K.; Battrell, C. Frederick; Bouzek, Heather K., Device for preparation and analysis of nucleic acids.
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Kolb, Andrew; Sprague, Isaac; Kay, Justin L.; Bragd, Matthew Scott; Stone, Martha, Fluidic circuits and related manufacturing methods.
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O\Connell James P. (Chapel Hill NC) Hoke Randal A. (Carrboro NC) Mize Patrick D. (Durham NC), Immunoassay using complement.
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Freytag J. William (Wilmington DE), Immunoassay wherein labeled antibody is displaced from immobilized analyte-analogue.
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Geisberg Mark S., Internally referenced competitive assays.
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Geisberg Mark S., Internally referenced competitive assays.
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Geisberg, Mark S., Internally referenced competitive assays.
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Mark S. Geisberg, Internally referenced competitive assays.
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Gefter Malcolm L. (Newton MA), Ligand assay with one or two particulate reagents and filter.
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Cocola Francesco (Siena ITX) Tarli Paolo (Monteriggioni ITX) Neri Paolo (Siena ITX), Method and composition for determining human chorionic somatotropin.
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Arnquist David C. ; Barnes ; III Grady ; Button Richard D. ; Dunn Chadwick M. ; East ; Jr. Richard C. ; Fritchie Patrick P. ; Galitz Charles M. ; Gardner Gregory E. ; Grandone Cass J. ; Gray Robert C, Method for determination of item of interest in a sample.
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Nicolas Jean-Claude (Montpellier FRX) Terouanne Beatrice (Montpellier FRX) Descomps Bernard (Montpellier FRX) Crastes de Paulet Andre (Montpellier FRX), Method for the separation of a protein substance from a solution containing the same by affinity filtration and applicat.
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Arnquist David C. ; Barnes ; III Grady ; Dunn Chadwick M. ; East ; Jr. Richard C. ; Fritchie Patrick P. ; Gardner Gregory E. ; Grandone Cass J. ; Gray Robert C. ; Holen James T. ; McCoy Jimmy D. ; Mi, Method of performing a process for determining an item of interest in a sample.
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Battrell, C Frederick; Gerdes, John; Mordue, Stephen; Capodanno, Jason; Hoekstra, Denise Maxine; Williford, John R; Clemmens, John, Methods and devices for microfluidic point-of-care immunoassays.
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Bouzek, Heather K., Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions.
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Clemmens, John; Battrell, C. Frederick; Gerdes, John; Hoekstra, Denise Maxine, Microfluidic mixing and analytical apparatus.
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Battrell, C. Frederick; Clemmens, John; Sprague, Isaac, Microfluidic reactor system.
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Bieniarz, Christopher; Ashworth-Sharpe, Julia; Kernag, Casey A.; Kosmeder, Jerome W.; Lefever, Mark, Molecular conjugate.
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Bieniarz, Christopher; Ashworth-Sharpe, Julia; Kernag, Casey A.; Kosmeder, Jerome W.; Lefever, Mark, Molecular conjugate.
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Moroz Chaya (40 Yehuda Hanassi Street Tel Aviv ILX), Monoclonal antibodies to placental isoferritin for use in detecting oncofetal ferritin associated with breast cancer and.
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Battrell, C. Frederick; Sprague, Isaac; Bragd, Matthew Scott; Capodanno, Jason, Sample-to-answer microfluidic cartridge.
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Arnquist, David C.; Barnes, III, Grady; Button, Richard D.; Dunn, Chadwick M.; East, Jr., Richard C.; Fritchie, Patrick P.; Galitz, Charles M.; Gardner, Gregory E.; Grandone, Cass J.; Gray, Robert C., Structure for determination of item of interest in a sample.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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[미국특허] Competitive enzyme-linked immunoassay 원문보기