초록 ▼

The process for freezing cell suspensions by locating the suspension in a freezing chamber and simultaneously monitoring the temperature of the suspended cells and of the chamber. The cooling of the chamber is regulated at predetermined rates in response to give temperature levels of the sample. The

The process for freezing cell suspensions by locating the suspension in a freezing chamber and simultaneously monitoring the temperature of the suspended cells and of the chamber. The cooling of the chamber is regulated at predetermined rates in response to give temperature levels of the sample. The cooling chamber includes a fan, a heater, and a source of refrigerant. The process includes the steps of selectively decreasing and increasing the temperature of the freezing chamber responsive to predetermined temperature points on the freezing curve of the cell sample.

대표청구항 ▼

The process for freezing cell suspensions in a freezing chamber using gaseous or vaporizing cold nitrogen for heat transfer comprising the steps of locating samples of the cell suspension in a container of fixed shape, placing at least one of said containers in the freezing chamber, simultaneously m

The process for freezing cell suspensions in a freezing chamber using gaseous or vaporizing cold nitrogen for heat transfer comprising the steps of locating samples of the cell suspension in a container of fixed shape, placing at least one of said containers in the freezing chamber, simultaneously monitoring the temperature of the cells in at least one container for detection of the supercooling, the freezing temperature and the length of the freeze plateau, and the temperature of the chamber, regulating the cooling of the chamber at predetermined rates automatically in response to the supercooling, the freezing temperature, the length of the freeze plateau, mass and geometry and heat transfer properties inside and outside the sample containers, including quick rewarming of the chamber after the end of the plateau phase during freezing, generating a homogeneous temperature field inside the sample containers so that each single volume element of the sample is cooled linearly and for a defined length of time, and providing a substantially small temperature deviation from the mean temperature at any point inside the sample containers during freezing by respectively controlling the cooling and warming of said chamber.