Method and test composition for determination of hydrogen peroxide
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/26
C12Q-001/28
C12Q-001/44
C12Q-001/48
C12Q-001/54
C12Q-001/60
C12Q-001/62
출원번호
US-0288123
(1981-07-29)
우선권정보
JP-0103665 (1980-07-30)
발명자
/ 주소
Miike, Akira
Shimizu, Yoshiaki
Tatano, Toshio
Watanabe, Katsuyuki
출원인 / 주소
Kyowa Hakko Kogyo Co., Ltd.
대리인 / 주소
Fitzpatrick, Cella, Harper & Scinto
인용정보
피인용 횟수 :
47인용 특허 :
3
초록▼
Disclosed is a method for the colorimetric determination of hydrogen peroxide in a sample by reacting a particular chromogen with the hydrogen peroxide in the presence of peroxidase and measuring the absorbancy of the reaction solution in the visible ray region. Also disclosed is a test composition
Disclosed is a method for the colorimetric determination of hydrogen peroxide in a sample by reacting a particular chromogen with the hydrogen peroxide in the presence of peroxidase and measuring the absorbancy of the reaction solution in the visible ray region. Also disclosed is a test composition for carrying out the determination.
대표청구항▼
1. A method for the determination of hydrogen peroxide in a sample which comprises reacting the hydrogen peroxide with a chromogen represented by the formulae ##STR## wherein Z represents hydroxyl, amino or substituted amino, Y represents oxygen atom or sulfur atom, R 1 represents hydrogen, alkyl, a
1. A method for the determination of hydrogen peroxide in a sample which comprises reacting the hydrogen peroxide with a chromogen represented by the formulae ##STR## wherein Z represents hydroxyl, amino or substituted amino, Y represents oxygen atom or sulfur atom, R 1 represents hydrogen, alkyl, alkenyl, aryl, amino or mono-substituted amino, R 2 represents hydrogen, hydroxyl, alkyl, alkenyl, aryl, amino, alkyl-amino or alkoxy, R 3, R 4, R 5 and R 6 represent hydrogen, alkyl, alkenyl, acyl, aryl, halogen, sulfo, nitro, carboxyl, hydroxyl, hydroxyalkyl or alkoxy, R 3 and R 4 or R 5 and R 6 may form alkenylene, X represents --S--, --O--, ##STR## R 7 and R 8 represent hydrogen, alkyl, alkenyl or aryl, in the presence of peroxidase and measuring the absorbancy of the reaction solution in the visible ray region. 2. A method according to claim 1, wherein Z is a dimethylamino group. 3. A method according to claim 1, wherein R 2 is a dimethylamino group. 4. A method according to claim 1, wherein both Z and R 2 are dimethylamino group, R 1 is a substituted amino group and R 3, R 4, R 5 and R 6 are hydrogen. 5. A method according to claim 1, wherein said hydrogen peroxide is a product formed by enzymatic reaction. 6. A method according to claim 5, wherein said enzymatic reaction is the oxidation of a substrate using oxidase. 7. A method according to claim 6, wherein said oxidase is selected from the group consisting of uricase, cholesterol oxidase, xanthin oxidase, choline oxidase, pyruvate oxidase, glycerin-3-phosphate oxidase, acyl Co A oxidase, glycerol oxidase, glucose oxidase and galactose oxidase. 8. A method according to claim 5, wherein said hydrogen peroxide-producing reaction and the reaction of hydrogen peroxide with said chromogen are conducted simultaneously. 9. A method according to claim 1, wherein said reaction is carried out in a buffer solution. 10. A test composition for the guantitative determination of hydrogen peroxide in a sample which comprises a sufficient amount of peroxidase and a chromogen defined in claim 1 to react with said hydrogen peroxide and form a pigment proportional to the amount of hydrogen peroxide in said sample. 11. A test composition according to claim 10 wherein said composition further a contains buffer reagent. 12. A test composition according to claim 10, wherein said composition further contains a member selected from the group consisting of a surfactant, antiseptics and ascorbate oxidase. 13. A test composition according to claim 10, which additionally comprises an enzymatic hydrogen peroxide-producing system in an amount sufficient to produce hydrogen peroxide proportional to the amount of a substrate in said sample. 14. A test composition according to claim 13, wherein said enzymatic hydrogen peroxide-producing system includes an oxidase for said substrate.
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