초록

A bright field light microscopic method for enumerating and characterizing subtypes of white blood cells and their precursors. The cells are labelled either directly or indirectly with gold-labelled antibodies in the presence of an inhibitor of oxidative phosphorylation.

대표청구항 ▼

A bright field light microscopic ethod for the quantitative determination and characterization of white blood cells and precursors thereof, which comprises the steps of (i) labeling viable cells of the desired subtype by allowing them to react first with specific non-labeled antibodies and thereafte

A bright field light microscopic ethod for the quantitative determination and characterization of white blood cells and precursors thereof, which comprises the steps of (i) labeling viable cells of the desired subtype by allowing them to react first with specific non-labeled antibodies and thereafter with appropriate gold-labeled secondary antibodies, whereby the cells are, until fixation, allowed to undergo extensive patching of the antigens, but are prevented from capping and all forms of internalization by keeping them in contact with an effective concentration of an appropriate inhibitor of oxidative phosphorylation; (ii) fixing the cells and staining the cells with endogenous peroxidase activity by contacting them with an appropriate peroxidase-indicator; and (iii) counting and identifying the gold-labeled cells in a given sample under the bright field light microscope.